Ptpmt1 (NM_025576) Mouse Untagged Clone

CAT#: MC204057

Ptpmt1 (untagged) - Mouse protein tyrosine phosphatase, mitochondrial 1 (Ptpmt1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptpmt1
• Synonyms: 1110001D10Rik; 2810004N20Rik; PLIP
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026750
  CCCGACCCCTCGCCGCGCCTCGCGGCGCCTCTCCTTTGGAGCCATGGCTCCTGTTCCGGGATCCCTCGGG CAGGGCCGGGACTCCGGGGACTCAGCTTCGAAGAGTCGGGAGGCATCAGGTGGCCCTCAGCTCTCGTCCT CCGCGTCCTTCTCGCGGTGGCTGGTCGCGAGCCCCGGGGCCGGTGGCTGGCCGCTGCGCCTGGCGGGGTG GGGCGCCTCGCCACTGCGCCTGGCGGGGTGGGGCGGGATGGCGGCCTCCGCGTGGCTGGAGGCCGGCCTG GCCCGGGTGCTCTTCTACCCGACGCTGCTCTACACAGTGTTCCGGGGGAGGGTGCGCGGCCCGGCGCACC GCGACTGGTACCACCGCATCGACCACACGGTTCTGCTGGGCGCGCTGCCGCTGAAGAACATGACGCGCCG GCTGGTACTGGACGAGAACGTGCGCGGGGTGATCACTATGAACGAGGAGTACGAGACCCGATTCCTGTGC AACACCTCGAAGGAATGGAAGAAAGCAGGAGTTGAGCAGCTACGGCTCAGCACAGTCGACATGACTGGGG TCCCAACCTTGGCCAATCTCCACAAAGGAGTCCAGTTTGCTCTCAAGTACCAGGCACTGGGCCAGTGTGT CTATGTGCATTGTAAGGCTGGTCGATCCAGAAGTGCCACAATGGTGGCAGCCTATCTGATTCAGGTACAC AACTGGAGCCCAGAGGAGGCTATAGAAGCGATCGCCAAAATCCGGTCACACATCTCCATCAGGCCCAGCC AGCTGGAAGTTCTCAAAGAGTTCCACAAGGAGATCACTGCAAGGGCAGCAAAGAATTAACTACTGATTAT CTCTCATGCTGACTTAAGAGTCTGAAGACTGTGGATAACAAAGAACTTGGATCTCCCTGAGATATAGTAA CAAGTTTACAGGACAAAGCAGCTTAACCCGACCACACTGCAAATGTGCTGACTGGTAGATAATGTGTCTC CTTTGCCTTCCTTCCTGAAATAACACTTGGATGGCTACAGAGAACAAGATTGTCTGGCTAAATCTTATTT TCTTTGACAGGGATAAGGAAATATTAATTTTAACAGAGATCTGTTGTTTCTAATGTGTGTAATGGCACTG TAAATCAAAGACATTTAATCCTAGAGAGGGAAAGAAGTATCTAGAGCTGTTCTTATTACTGTCAGAATCC AAATCTTAATTGTATGACTTGGCAAAGTGCTTGCCCAGCATGTATAATAAGGCCCCAATTTGGTCCCTAG CAGGTCCAAAAATAAAAATAAAAATACAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025576
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026750, AAH26750
• RefSeq Size: 1306 bp
• RefSeq ORF: 786 bp
• Locus ID: 66461
• UniProt ID: Q66GT5
• Cytogenetics: 2 E1
• Gene Summary: Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG) (PubMed:21641550, PubMed:21730175). PGP is an essential intermediate in the biosynthetic pathway of cardiolipin, a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle (PubMed:21641550). Has also been shown to display phosphatase activity toward phosphoprotein substrates, specifically mediates dephosphorylation of mitochondrial proteins, thereby playing an essential role in ATP production (By similarity). Has probably a preference for proteins phosphorylated on Ser and/or Thr residues compared to proteins phosphorylated on Tyr residues (By similarity). Probably involved in regulation of insulin secretion in pancreatic beta cells (By similarity). May prevent intrinsic apoptosis, probably by regulating mitochondrial membrane integrity (By similarity).[UniProtKB/Swiss-Prot Function]