Gal3st1 (NM_016922) Mouse Untagged Clone

CAT#: MC204018

Gal3st1 (untagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gal3st1
• Synonyms: Cst; Gcst
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026806
  GGCATGGACCAGGCCTGCCAGCGCGCCGGGTCCCGGCGAGTGCGCCCCGCCGCCCAGTGAGCGCCACGGC GGCGGCGGGGGCCGGGGCCGACCCCTGCCACCCGGAGCCCAAGGAGAACAGGGCAAAGTGCCTCTCACTG GGCTCCAAGGAAGAACAAACAAGCACTTGCAGGAAAGCAGTGACTCACAGGACAATGGCAATGGGGGACT CTAGGCCCCCTCAGTGGCTTGGGGCAAGCTTCTTGCTTTGGTTTGACTCCATGAAATGTGAATGACTAGA GAAGACACAGCGGGATGGAGCAGAAGTACCCCAAAGGTTACCAGACTGGAGACCTGGAGTCATCAGGGGT TTCCTGAGATGACTCTGCTGCCAAAGAAGCCCTGCAAGTCCAAGGCCAAGGGGTTACTGCTGGGAGCTCT CTTCACCAGTTTCCTATTGCTGCTGTACTCCTATGTGGTACCTCCACTGTATCCCAACATGGCCTTCACG ACCTCAGAGGCTGCAGCACCCTGCTCCCCTATTCCCAATGAGCCAGTGGCAGCCACTCCCGCCAACGGCT CAGCAGGAGGGTGTCAACCTCGGCGAGATATTGTGTTCATGAAGACGCACAAGACCGCCAGCAGCACACT GCTCAACATCCTGTTCCGCTTCGGCCAGAAGCACGAGCTCAAGTTCGCTTTCCCCAATGGCCGAAACGAC TTCCACTACCCGTCGTACTTTGCACGAAGCCTGGTGCAGGACTACCGGCCTGGGGCGTGCTTCAACATCA TCTGCAACCACATGCGCTTCCACTATGAGGAAGTGCGCGGGCTGGTGCGGCCGGGTGCCACCTTCATCAC CGTCATCCGCGACCCTGCTCGTCTCTTCGAGTCTTCCTTCCACTACTTTGGATCCGTGGTGCCGCTCACC TGGAAGCTGTCGAGCCGCGACAAGCTGGCCGAGTTCCTGCAGGACCCCGATCGCTACTACGACCCGAGCA GCTACAACGCGCACTACCTCCGCAACCTGCTCTTCTTCGATCTGGGCTATGATAGTAGCCTGGACCCGGC CAGCCCGCGCGTGCAGGAGCACATCCTGGAGGTGGAGCGCCGCTTCCACTTGGTGCTACTGCAGGAGTAC TTCGACGAATCCCTGGTGCTGCTCCGAGAGCTGCTGTGCTGGGACCTGGAGGACGTGCTGTATTTCAAGC TCAATGCGCGGCGCGACTCGCCGGTTCCGCGGCTCTCTGGCGAGCTGTACCGCCGAGCCACCGCCTGGAA CCTGCTGGACGTGCGCCTCTACCGTCACTTCAACGCCAGCTTCTGGCGAAAGGTGGAAGCTTTCGGGCGT GAGCGCATGGCGCGCGAGGTAGCCGAGCTGCGCCAAGCGAACGAGCACATGCGCCACATCTGCATAGATG GCGGCCAAGCAGTTGGTGCTGAGGCCATCCAGGACTCGGCCATGCAGCCCTGGCAACCCCTGGGCATCAA GTCCATCCTGGGTTACAACCTCAAGAAGAGCATCGGGCCACAACACGAGCAGCTCTGCCGGCGCATGCTC ACGCCCGAAATCCAGTACCTGTCTGACCTCGGTGCCAATCTCTGGGTCACCAAGCTCTGGAAGTTCCTTA GGGACTTTCTAAGGTGGTGATGTATCCTGCCCACCTCCTGTTTGGCTTCCTGATGCAGCAAGGCCGGGCA GGGAGCCTCTGGCACCTAGCCTTCCCTAGCCACACCTAGTGCCACCCTGGGGTCCCTAGGGTCATAGCTG TCTGGGACTAGTCTCTCTGCAGGAGAGAGCCTAACGGAGAGGTATTTAACTAATTATGCCATTTTTTTTT TATTAAATCCCCTTTATTTCCAGCCTCCTCTTACAAGGGGAGACGCAGAAGTAAAGAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_016922
• Insert Size: 1272 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026806, AAH26806
• RefSeq Size: 1918 bp
• RefSeq ORF: 1272 bp
• Locus ID: 53897
• UniProt ID: Q9JHE4
• Cytogenetics: 11 A1
• Gene Summary: Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of HSO3-3-galactosylceramide (sulfatide), a major lipid component of the myelin sheath and of HSO3-3-monogalactosylalkylacylglycerol (seminolipid), present in spermatocytes. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1, also known as form A) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. An in-frame AUG is located 29 codons upstream of the annotated translation start site but is not being annotated as a start site since it would be inhibited by an overlapping uORF with a strong Kozak sequence. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.