Dap3 (NM_022994) Mouse Untagged Clone

CAT#: MC203985

Dap3 (untagged) - Mouse death associated protein 3 (Dap3), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dap3
• Synonyms: 4921514D13Rik; DAP-3; MRP-S29; S29mt
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019566
  GCCACGGCTGCCGCCGCCTTCGCCCCCACCCCTCGAACGTGCGCCTCCCACAATCCCCCCCGCGGGACGT TCCATTGCGCGCCCCTGCTGGGGCCGGGGAGAAGCGGGCACGCCCGCGCAGACGAGGCTGGCCACGGTGA CCGGAATGTTTTGAAGAGCGAGCGAAGGTACCTAGGCGCGGACCTCCTGGGACGGGCCACCGCGACGCCG AGGCTTGCTGCCCGCTTCCGGCCGCGGGGCGGGTGGCGCGGTGCCCCTCCGCTGGGCTGTGGTTGCAGCC GCGGCGACTGCGCGTTGGGTAGCTTCCTGCCACACGACCCGAGCTTGCTTCACAACTGCAGGACGGGTGC CTGGGATCCAGCCGCTGGCGGTGTGTGCGGGTCACCGGAGCAGGAGTCCTAGTGTTGGACTCATGAGAGC AAAGACCATGCTGACAGGAATAACAAGGCTTTTCTCCAGGGTCCAGAAGTTGGACCCGAGGTGTTTTTTG CACATGAGTGTTCAAGCGACTCAGAACAGTCAGGTTCCAGCTGAGCGTCCCAGAACTGTTTCCCGCACCA GTGACAGTGACCCGGCTAAGCATGGGGAGCAGCATGAGGGTCAGCACTACAGCATCCCCCTCCAGGATCT GAAGACAGTGTTTCCTCACGGCCTGCCTCCTCGGTACATGATGCAGGTGAAGACTTTTGGCGAAGCTTGT CTGATGGTCAGGAAGCCAGCTCTCGAGCTTCTGGGCTACCTGAAAAATACCAATTTTGCTCATCCAGCTG TGCGATATCTTCTTTATGGGGAGAAGGGGACAGGAAAGACCCTCAGTCTCTGCCATGCTGTTCATTTTTG TGCAAGACATGACTGGCTGATTCTGCATATCCCAGATGCTCATCTTTGGGTAAAAAACTGCCGGGAGCTT CTACAGTCCACTCACAACAAACAGCGCTTTGATCAACCCTTAGAGGCCTCTACCTGGCTGAAGAATTTCA AAACTACCAATGAGCGCTTCCTGAGTCAGATAAAAGTTCAAGAGAAGTATGTTTGGAATAAGAGAGAAAG CACTGAGAAAGGCAGTCCTCTGGGAGAAGTTGTTGAACAGGGCCTAACCCGAGTGAGGAATGCCACTGAC GCTGTTGGGGTCGTGCTGAAGGAGCTGAAGGCTCAGAGCGCCCTAGGGCTCTTTCACCTCCTGGTGGCTG TGGATGGAGTCAATGCCCTCTGGGGAAGGACCACACTGAAAAAAGAAGATAGAACCTTGATTGCCCCAGA GGAACTCTCCCTTGTCCACAATCTAAGGAAAATGGTGAAAAATGACTGGCATGGAGGTGCAATCGTGTTG AGCTTGAGCCAAACCGGGTCTCTCTTTAAGTCCAGAACAGCCTATTTGCCGCATGAGCTGCTGGGAAAGG AAGGATTTAATGCCCTGGAGCCTTTTCTTCCCATCCTCATTCCCAACTATAATCCAAAGGAGTTTGAAAG TTCCTTCCAGTACTACTTAGAGAACAACTGGCTTCAACATGAGAAAGCTTCAACAGAAGAAGGGAGGAAG GAGCTGAGGTTCCTGAGTAACTGCAACCCTGAGCAGCTGGAGCGGCTCTGTGCCTCGCTGTAAGCACGGA CAGCGGCGGTGGCATGCTGGGAAGACAGGCTTCCTTCAGCTACCGTGCGCGCATGAGGAAGTCCCAAGGT TCCAAGCTGAACACCCTGACCTGCACTGATGGATATGAAATGATAGGATGTTGTTGTCCTACAGCTAACA CCAGTTTTCTGTTCGAGTTTTTACATTAAAGTAGATCTGCTCCTGAGTGCTGTATTGGATTTGCAACAAG GAAGGTTCTCACCCTGGCCAGACTCTGTCAGTTTCTCTGGTGTTCTGTTTGCTGGCATCTGAAGAAACTC AGTTCTACAGAAATTAGTAGTTCTGTCTCCTGGATTTAAATCTGTTCAGGAGACCAAGATTTACAGCCGA ATTCCTAATAAGACAGGTAACGCTTCTCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_022994
• Insert Size: 1176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019566, AAH19566
• RefSeq Size: 2003 bp
• RefSeq ORF: 1176 bp
• Locus ID: 65111
• UniProt ID: Q9ER88
• Cytogenetics: 3 39.01 cM
• Gene Summary: Involved in mediating interferon-gamma-induced cell death.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to extend the N-terminus. The use of an alternative upstream start codon, which appears to be rodent-specific, would result in a protein that is 5 aa longer at the N-terminal. The second AUG, 5 aa downstream the rodent start codon, which is better conserved in mammals, could be used at least some of the time.