Orc2 (NM_008765) Mouse Untagged Clone

CAT#: MC203848

Orc2 (untagged) - Mouse origin recognition complex, subunit 2 (Orc2), transcript variant a, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Orc2
• Synonyms: AU041563; Orc2l
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015257
  CGCAGGTTCGGCTTGGACCGGTCGACTCTTTCCCTAGACGCTGGCCTCCCCGGGCCGTTCTGGGGTGTTC CGCGGCGGCGGGAGTCCTGGCTGGCGGCTCAAGTGAGCCTCGCGGCCGTTCTCCCTCCCGCCGGCTCGGA CTCGAGAGTTACAGGTCACTGGATGTTCAAGCTGCAGAATGCATAGCACCTTACAAGGTTGGCCACAATG AGCACTCTGCAGTTAAAGGAAACCAAGGTGCCAAGCGTGCAGTTTGTGGGAGATGATGATGTTCTTAGTC ACATTCTGGACAGAGAAGGAGGAACTAAATTAAAGAAGGAGAAAGCTCAGCTTTTGGTCAATCCCCAGAA AGTAATAAAGAAGGCAGACTGTGAGTTGGAGAAAAGTGACCTGGAAGTTTTAGAGGATCAGAACTACGTG AAAGTCTTGGGAAGAAATATTCAAGAATCCTTGGGAAACGGCTCTGCTAAAGATGGTAGGAATAAAGTTT ATTCTTTTCAACAAAGAAAGCACCCTGAAGAGATGACTAAATTGGCTTTAGAACTAGCAAAAACATCAGG AAAAAAGGATCCACTTGACTCAAATGATCCTGAAATCACAAAAAACATTGCTCAAAAAAGTAAAGGGCAT TCTACTTCAGAGAAAGCTCCACTGGTGAACAATAACAAAACTGAATTTCTGTCAACACAACCTCATAATC TAAGGAAAAGAATAATAGCGTCAAGGTCTCATTATGACAGTGAGAGTGAGTATTCTGCTTCCAGCTCAGA GGATGATGAAGAGGCTACAAAAGACGAAGAAGAAGACACTAATGTGGCCAGGCTCTCTCAGAAGAGTCAA GGTCAGAATAGACTTCTTCCAGCTCCTGTTTCCAAAGAGACACTGCCTAAGAAAAAGAAAAGAGACAAAG CGAGTGACTTGGTAGAAGAATATTTTGAAGCCCATAGTAGTTCAAAAGTCTTAACATCTGATCGAACGCT GCAGAGGCTGAGGAGAGCCAGAGTGGATCAGAAAACTCTGCATAACTTACTGAGAAAATTTGTGCCTTCC TTTTCTGCTGAAATTGAACGATTGAATCAACAGCATGAGAAACTGTTTCATAAATGGATGCTTCAGTTAC ACCTTGGGTTCAACATTGTGCTTTATGGCTTGGGGTCCAAGAGAGATTTACTAGAAAAGTTTCGAACCAC CATGCTTCAAGATTCCATTCATGTTGTCATCAACGGCTACTTTCCTGGCGTCAGTGTGAAATCAATCCTG AACTCTATAACTGAAGATGTCCTCAGTCATGTGGGTACTTTCCAAAGTGTTCTGGATCAGCGGGACTGGA TAATAAACAGATTTAAAGAAGATTCTTCTTTAGAACTCTTCCTTCTTATCCACAATTTGGATAGCCAAAT GTTAAGAGGAGACAATAGCCAGCAAATTCTTGGACAGTTGTCATCATTACATAACGTGTATCTCATAGCA TCTATTGACCATCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTATAACTGGCTTTGGT ATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCCCTTCTGGTTAAGCAGTC TGGATCTCTGCCACTTAGTTCTCTGATTCATGTCTTACGAAGCCTTACCCCCAATGCAAGGGGGATTTTC AGGCTACTTATGAAGTTCCAGCTGGATAACCAGGACAGCCCTTCCTACATTGGACTTTCTTTTCAAGATT TTTACCAGCAGTGTAGGGAAGCATTCCTTGTTAACAGTGATCTCACACTCCGAGCCCAGTTAACTGAATT TAGGGACCACAAACTTATAAGAACAAAGAAGGGAACTGATGGTGTAGAATATTTATTAATTCCTGTTGAC AGCGGAATATTGGCTGATTTCTTGGAGAAGGAAGAGGAGGAGGCATAGGCTGTCTCTTATCCTTGAACTT TCTGTGGAAGCCTCATTGTGGCCCAGCTGCTGCTCCTCAGTAAGAATGCTGGTTTACATCCAACATTAGG TCATTCTCTCCAGCATACAAGATGTTCTATGGAAGAGGCATAGCATCAATTTGAACTTTGATTAGCCTGG CTGGTGTACTGTCTCTAATACTATGCAGTGGTCGTTGAGTTGGATAAAAGTGCCTTTAATTTATTACTTC TCTGGAGACTTCTTGCTGGTTGTACAGTGTGCTCAGGGATTCTTTGGAACTGGATGGTTTTGGGTTATTT TATACTGAATGCTATCCCTAATTTTTGAGGGCAATTTAACACTCCAGAGCTGAATTACTACAAGTGCACT TGCTTCAGACGGTCAGAGGACAAGAGTTCAGAGTAGCGTTCTCACGGGACTGATGAGGAAGGAGGTGTCC TGCTTCTCCTGAGATGATGGTAGAAGATGTCAGTGGACTGAGATGACACAGCCTTCTCCTTGTGGACTTA GGCCACACATGCTAAATAAAGACAGCCAGCACTGTTCAAATGAGATCACGGGAGAAGTACAAGGGATGTG TGCATATGTGGAGCATGTGTCCAATCTAGAAAGCATATTTAAAATCGTTGGTATCGGTACCAGGAGAAGC CAGGCTAAGAAATAAAGTCCTGATTTACATTTTATTGTTTTCAGTACAGTAGAAAATAAAAACATTTCAT CATTCTTGTACATTGACATTTTCTATTTTCTTAAATAGCTCACAGGGATAGGTAGCGCACACCTTTAATC CCAGCACTCAGGAGTCAGAGGCAGACGGATTGCTTCTCTCCTTTAAGACACAAGACCTGAGCTCACATTT GTAATACCAACATCAAGAAGGCAGAGGCAGAGGAAAACATGAGTTCAAGACCTAGACTAGTTAGTGAGTT CTAGACCAGTCTAAGCTGCGGTGATGTCCTGTTTCAAAAACAAACATTCAGTAAATAAATACACATTGGT AACATGAAACCTGCTGGTCTGCACTTTGCTTGTGTGGAATGTGGTATGTGGGTGTCTGTGCAGAGACTGA GTAAGATGTCTTCTACCACCTTCTGCCTTATTTCCTTGAGACAAGGCCTCTCTTCACTGAACCAGAGCTC CTTGTAGGCCAGATGGGCTGCCAGTGATCCTGTCTTCACACCAGTACTGGTGTGACAGGCACAGCCATGC CCAGCTTTTTGTGAGTGCTGGGATCCAAACTCAGGTACTTCTTACCCACTGAGACATCCCCCCAACTCCA TGTACTTTCACAGTTTTGCTTAATTTCTCTAGTGTAACTCCTACAGTATAAACAAACATCTGATTATAGA ATGTGACATCTTTGCAGTTGCCTTTATAGTTTTTGTTATTAGATGTCGCTCTTAATAAATCAAAGTGCAG TTTGCAGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008765
• Insert Size: 1731 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015257, AAH15257
• RefSeq Size: 3417 bp
• RefSeq ORF: 1731 bp
• Locus ID: 18393
• UniProt ID: Q60862
• Cytogenetics: 1 C1.3
• Gene Summary: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (a) represents the longest transcript and encodes the longest isoform (A). Both variants a and c encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.