Nfu1 (NM_020045) Mouse Untagged Clone

CAT#: MC203723

Nfu1 (untagged) - Mouse NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) (Nfu1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfu1
• Synonyms: 0610006G17Rik; CGI-33; Hirip5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC018355
  GCGGGCATGGGGAGCAGCGGTCGGTGTAGTCCGGCTGTGCAGGCGGTTCTGTCATGTAGCGACTCCACAC ACCTTTAAGAAACAACCTCTGCATCAATATGCACGGAGACCACTTTTCCCGCTGCGCGCACCCTTGTGTA ACACAGTACGATTCATGTTTATTCAAACACAAGACACCCCAAATCCCAACAGCTTAAAGTTTATACCAGG AAAGCCGGTTCTTGAGACGAGGACCATGGATTTCCCCACCCCTGCTGCAGCTTTCCGCTCCCCTCTGGCC AGGCAGTTATTCAGAATTGAAGGTGTGAAAAGCGTCTTCTTCGGACCAGATTTCATCACAGTCACAAAGG AGAATGAAGAGTTAGACTGGAATTTACTGAAACCAGACATTTATGCCACAATTATGGACTTCTTTGCATC TGGCTTACCCCTAGTGACCGAGGAGACGCCTCCGCCTCCGGGAGAAGCAGGCTCTGAGGAAGATGACGAA GTCGTGGCAATGATTAAAGAACTGCTAGATACTAGAATAAGGCCAACTGTGCAGGAGGATGGGGGCGACG TGATCTACAGGGGCTTTGAAGATGGCATCGTGCGGCTGAAGCTCCAGGGCTCCTGCACCAGCTGCCCCAG CTCCATCATCACCCTGAAGAGCGGGATTCAGAACATGCTGCAGTTTTATATTCCAGAAGTGGAAGGTGTG GAGCAGGTGATGGATGATGACGAATCAGATGAAAAAGAAGCAAATTCATCTTAAATAATCGGCATTTTCT TTGGCCACAACCATTGGACTTATCTTTACTGATCCTGTAATTATAGTCTCTGCTATGGAAGTTTATTAAT AAAAGGTTTCCTTCCTCCAGAATGATATAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_020045
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018355, AAH18355
• RefSeq Size: 885 bp
• RefSeq ORF: 600 bp
• Locus ID: 56748
• UniProt ID: Q9QZ23
• Cytogenetics: 6 D1
• Gene Summary: Iron-sulfur cluster scaffold protein which can assemble [4Fe-4S] clusters and deliver them to target proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the coding region, compared to variant 1, which results in a protein (isoform 2) that is one amino acid shorter than isoform 1.