Trim30a (NM_009099) Mouse Untagged Clone

CAT#: MC203672

Trim30a (untagged) - Mouse tripartite motif-containing 30A (Trim30a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim30a
• Synonyms: Rpt-1; Rpt1; Trim30
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005447
  GGCCTGTGTTTGCTCCTCTCCACAGATCCTGAGTCTCTTGGTTGGTATCTGAAGATGTGAGCCAATTTTC AACTGCAGAGTTTGGAGGAATTTAGAAAAAGGCACAGTCTTTGCTCTGCAGTGTGAGGAGTCAGGACAGG CAGACACAGGATCACAGCAACTATGGCCTCATCAGTCCTGGAGATGATAAAGGAGGAAGTAACCTGTCCT ATCTGTTTGGAGCTCCTGAAGGAACCTGTGAGTGCTGATTGTAACCACAGCTTCTGCAGAGCCTGCATCA CACTGAATTATGAGTCCAACAGAAACACAGACGGGAAGGGCAACTGCCCTGTGTGCCGAGTTCCTTACCC ATTTGGGAATCTGAAGCCTAATCTACATGTGGCCAACATAGTAGAGAGGCTCAAGGGATTCAAGTCCATT CCAGAGGAGGAGCAGAAGGTGAATATCTGTGCACAACATGGAGAGAAACTCCGGCTCTTCTGTAGGAAGG ACATGATGGTCATCTGCTGGCTTTGTGAGCGATCTCAGGAGCACCGTGGTCACCAAACAGCTCTCATTGA AGAGGTTGACCAAGAATACAAGGAGAAGCTGCAGGGAGCTCTGCGGAAGCTGATGAAAAAGGCAAAAATA TGTGATGAATGGCAGGATGACCTTCAACTGCAGAGAGTTGACTGGGAGAACCAAATACAGATCAATGTAG AAAATGTTCAGAGACAGTTTAAAGGACTAAGAGACCTCCTGGACTCCAAGGAGAATGAGGAGCTGCAGAA GCTGAAGAAAGAGAAGAAAGAGGTTATGGAAAAGCTGGAAGAGTCTGAAAATGAGCTGGAGGATCAGACA GAGTTGGTGAGAGACCTCATCTCAGATGTGGAACATCATTTGCAGCTCTCAACCTTAGAAATGCTGCAGG GTGCAAATTGTGTCCTGAGAAGGAGTCAGTCCTTAAGCCTGCAACAGCCCCAAAGTGTCCCCCAAAAGAG AAAAAGAACATTCCAAGCTCCAGATCTGAAAGGCATGCTGCAAGTGTATCAAGGACTCATGGATATCCAG CAATACTGGGTTCATATGACTCTACATGCAAGGAACAATGCAGTCATTGCCATTAACAAAGAAAAAAGAC AAATACAGTATAGAAGTTACAATACGGTTCCAGTTTCTGAGATCTACCATTTGGGTGTCCTGGGATATCC AGCTCTTTCCTCAGGGAAGCATTACTGGGAAGTAGACATATCTAGAAGTGATGCCTGGCTCCTCGGATTA AATGACGGAAAGTGTGCTCAACCCCAACTTCACTCAAAGGAAGAAATGGGCATCAAAAAAAACCTTCATT CTCAGATCAAACAAAATGTATTGTTTCAGCCTAAATGTGGCTACTGGGTTATAGGGATGAAGAATCCGTC TGTATACAATGCCTTTGATGAGTGTTCTATCACCCACAATTCCAGTATCCTGGTCATCTCTCTGCCTGAT TGTCCCAGTCGTGTCGGAGTTTTCCTGGATCGGAAAGCTGGCACTCTCTCATTTTATGATGTTTCTAACT GCGGTGCTCTCATCTATAGGTTCTATGACCCTGCCTTCCCTGTTGAAGTCTATCCATATTTTAATCCTAT GAAATGTTCAGAGCCAATGACTATATGCGGGCCACCCTCCTAAACCTTCACCTATATCTATATCTATATC TATCTATATCTATATCTATATCTATATCTATATCTATATCATCTATATCTTTATACAGTGCCCCCATGTC TGGTGTATCTTATGCAAGAAACCCCTTGCATCATTCTAACTTCTTTTTTGTCTTGGGCCTTTTATTTTAA TGACCTTCACTTCTAACCCCAAACCACTGTAGATTCATTTCCTTGATCCAGTTGCTTGTCTGTTCACAAG ATGATTACTGTCATGTATCATTTCCCATAAAAAAAAAAAAAAATAGTTGTTTCCTCACAAAGTAAAATAG ATCCTCTGCTAACACCATCTCTGTAGAAACACAGTCAAAAGAATGACTCATGGTGAGCAATGTCTTAGGG ACCTGTAAGAGAAGCCCCAGAGAAAAAGAAAGAGGGCCTTTAACCTCAGGGACATTTTGTTCCCTGGATT GCTTTGGATGTGATTTTGTGCCTCCTGCAAAAAGAACATCTGCATTCAATTTGTAAGATCTGTTTACTCT TGTTGGATGTCAGAACATAGCTATAGATCCAATTCCTGTGCAAGGATATACTGAATGCTGGCCTCAATGT ACAAATTCTGAATATGGACTTGTGCCTTGATCTCATTTTTTTCAAGATATCATCTATAGCATAGTACCAG ACACCTGTGTTTATATGGTCTGTCCTGAGACAGTTGTAGGAAAGGTTTGTTAATATGTTCTTACTGGTAT GTATTTCCATGTTTTTCTGATTTGGTTCTTCTGAACTCATCAACCTTCCTGCTTTCTTTTGTACATTCAT GTTTAAAAGCACAGTGAAACTGAAATAAAGTTGTCTGCAGCATCAGGCTGTAGTCCCCCCATTCTTTAAG GTCTGCAAACCCCAGATCTTACAGACAGAACTACATAGGCCCACAACGGTATGCACACCTTCTTTTTGGT GTTGCCAGAGATAGAAGCCAGGGATTCGTGAGTGCTTATGAGTTGCTCTACCCCTGAGTTACTTGTCTCA GCTCTGTCAGGTCTCCACAGCTGCATTCTTTCTGAAACTGAAAAAGCTGAGAGAAAGTTCTTCTCCAGTG TGTCTAGCAGTTTAGGAAATTATATGTAGGAGTCTGTCTTTAAGGAATGGGACTTAGCAGAAGGTCAGTT TAACCACAATGCTTGTGTTTCATCAAAAAGTTAAGAAGGAGACATCTCAAATGATATCCACTAAGACCAT GTGTTTAGAATACCCAGGTTCTAATCCTGTCTCTCCATATAGCATTACAACCCTGAATAACATCATCTCA AGCCTGTTTGTTTGTTCTTTTCTTTGTTTGTTTCATTTTTCTTTTTTGTTTTTCAGTTTTGCTAAAATAT CACACCTATATTACACGTCATTTTAGGAAAATAAATTTGAAATCTTTACAAGGCTCTGAGAGTAAAAGCC CTTTCCTCTGTGAGTGATGAACTGTGTTCATTTACTTAGACCCTCTTGGCTGAAGGAAGCAGCCAGCTCT GAGAGATGATCGTCTTACTTCACAAATATGCTACAGAACATCTAGGCTGAAACTCATACAAATGAGAGGA GACTATGTTATTTTTAGTAAGAAAAGATCAATAGGTATAATGTGTCCAGTGCACACTCTGTAAGTGGGGA TTGTGCTAATTACTGGGTAGATGTGCTGTACTTATGTTCTATGTGGCACTGAGGAAACTAAGGTATAGAT CTTCTCATGTCTGGATATTAGATCCTGGAAGCTGCCGCCAAGACTATTCTAATGTGTTGTGGATATATAT GATTTTTTTTCTTTTCACACTTACTTTTTGAAAGGATATTTGTTTAGCTGTATTCATTATCAAGTAAGTT AAAATCTATGATTTTCCATTCACAATAAATAATAATAATGACAATGATACCCATAACTGAACCTTCTGTT ATGTTGTGAAATGTAAACAATAGAATGTTTCTAGGAACTCATATTGCTTCTAGCAGATAGAAGGTATCAG ATACTGTATCTGTTAAGTTTTTTCTACCCTTTGTTGTGACCACAATAAAGTACCATTGGAATGACAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009099
• Insert Size: 1491 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005447, AAH05447
• RefSeq Size: 3738 bp
• RefSeq ORF: 1491 bp
• Locus ID: 20128
• UniProt ID: P15533
• Cytogenetics: 7 55.69 cM
• Gene Summary: Trans-acting factor that regulates gene expression of interleukin 2 receptor alpha chain. May affect IL2R-alpha expression through cis-acting negative regulatory elements or through competition with proteins that bind to enhancer or activator sequences. Negatively regulates Toll-like receptor (TLR)-mediated activation of NFKB by promoting degradation of TAB2 and TAB3 and preventing TRAF6 autoubiquitination. Negatively regulates production of reactive oxygen species (ROS) which inhibits activation of the NLRP3 inflammasome complex. This, in turn, regulates activation of CASP1 and subsequent cleavage of IL1B and IL18. No activity detected against a range of retroviruses including a number of lentiviruses, gammaretroviruses and betaretroviruses.[UniProtKB/Swiss-Prot Function]