Pus7 (NM_178403) Mouse Untagged Clone

CAT#: MC203665

Pus7 (untagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pus7
• Synonyms: C330017I15Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC008544
  GGCTGGTGGGATAACCCGTCCCTGTGCGTCTCCACGCGGTGCAGCCTGTGCTGGTCCGCGCTGGCCGCCG CCTCCTGCGAGTCACCGCCGCCGCCCTCCCGCGTCCCGAGCCGAGCGTCCTCTTCGCCGCTCGGCCCGCG CCCTCAGGCTCCCGGTTCTCTTAAATGTTTTCTTGGAGCCTTAAAGATGGAAATGACAAGCACGTCCCTG AAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCACACGACGAAACAAAGAAACAGAAGG TGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGACGGTCTACACCGCAGTGAGAACGA GCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAATTCCAGCGCTCAGGTGCAAGAGGAA GAGGAGGAGGAGGAGGAGGAAGACGGCCTTTCAGAAGCAGGGGAAGAGGAAGAAGCAGAGAGTTTTGCAG ACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAAGTTCGTGAGTTCCCATCATGGCTT CTCAGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAAATCGGGAAAGATGGACGGATCAGC CATTTGGATGACTTATCTGTTCCGGTGGACGAAGAGGACCCTCCAGAAGACGCTCTCACTGTCTTGACAG CTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAAAGAAACCAGTGTTGCCATCGAGGT CATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATTAAATCTCTCTTCCCAGGACTAGAG ACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACCATGCAGCTGGCAAAAAGGCTTTGG CAAATCCAAGAAAACATTCTTGGCCAAAATCCCGGGGAAGTTACTGCCATTTTGTTCTCTACAAGGAAAA CAAAGACACCATGGATGCCATCAATGTTCTATCCAAGTACCTGCGGGTAAAGCCAAACATATTCTCCTAT ATGGGAACCAAAGACAAAAGGGCAATCACAGTCCAGGAGATTGCTGTTCTCAAAATAAGTGCACAAAGAC TTGCCCACCTCAATAAGTGCCTGATGAACTTGAAGCTCGGGAATTTCAGCTATCAGAAAACCCCTCTGAA GTTAGGAGCACTCCAAGGAAACCACTTCACTGTTGTTCTCAGGAATATAACAGGAACTGATGAGCAGGTG CAGCAAGCCATGCAGTCTCTGAGGGAGACCGGCTTCATTAACTACTATGGCATGCAAAGGTTCGGGACCA CAGCTGTTCCAACGTACCAGGTTGGAAGAGCAATTCTACAGAATTCCTGGACAGAAGTCATGGATTTAAT ACTGAAACCCCGTTCTGGAGCTGAGAAGGGTTACTTGGTGAAATGCAGAGAAGAATGGGCCAAGACCAAA GACCCAGCCTCTGCTCTCAAAAAGCTGCCCGTCAAAAGGTGTGTGGAAGGGCAGCTGCTCCGAGGACTTT CCAGATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAATTCCAAGAAACAATCGCTTAATGTACAT CCACAGTTATCAGAGCTATGTGTGGAACACCATGGTGAGCAGAAGAATTGAGGAGTACGGGCTGAGGCCT GTGCCCGGGGACCTTGTTCTCAAAGGAGCCACGCCCACCTACATTGAGGAAGATGACGTTGACAACTACT CCATCCACGATGTGGTGATGCCTTTGCCTGGGTTTGATGTTATCTACCCCAAGCATAAAATCAGTGAGGC CTACAGGGAAATGCTTGCTGCAGACAACCTTGATATTGACAACATGAGACACACGATTCGAGATTACTCC TTATCGGGGGCCTACAGAAGGATCATCATCAGGCCTCAGAGCGTCAGCTGGGAAGTTGTTGCATATGATG ATCCTAAAATTCCACTTTTCAACACGGATGTGGACAACCTCGAGGGGAAACCACCACCAGTGTTTGCTTC TGAAGGCAAATACAGGGCTCTGAAGATGGACTTTTCTCTTCCTCCTTCTACTTACGCCACCATGGCCATT CGAGAAGTGCTCAAAATGGACACCAGCATCAAGAACCAGACCCAGCTAAATACGAGCTGGCTGCGATGAA CAGTGCATCGCTCAGAGAGGGGATGCAGACCTGCTTCTGACCTCCTATTCTGGTCTTTTTTTAAGTGCTG ACTCATGTTTGGAGTTTAAAACTTTTGTAGTAAAGGATGATTTGTATTTTGTAAAGTTTTTAATCAGCTT AAATACTTTGCAATATTTGTACACATATACAAGTCCTGAGGATCCTAATTTCAGCTCAGAGATAGAATTG GGTCAGCATATCCTGTAGGTGCTTAGATTCTAGTACAGCATCAGCTTCCTGCCTTTAACACAGGGAAGGG GGACTTGGTTTAAAGTTGCGGTTTTAGGTTTTGCTGCACACCTGAAGTACAAACCAGAATCTTAGATGTT TGTGATGAGATTACACATGGTCCGTGTCAGAGCAAATGCAGCAGACTGGAAACTAGAGACTGCACACTTA ACACGCAGTTCCCGTGTTGGTTTCAATAATAACAAAACATCCACGGGTTTATAAACACGGCGTGGCTTTT GTTGAGTGACTGTGTTAGCTTCAGGTGAGCATTTATACACATCTTAGCTTGCAGTGGCTTTTCTGGTTAG TCTATCAGCATCTTTCAGGGCAGTCTTTTCCTTGATAGGAGCCTGTAGCTTTTCTTGGGTACAGTCTGAT TTTACTCTGGGTGCCTCTTACTTTCAGAACCTTTTCACTGGACTCAAAAGTAAATAGTGTGAGTTCCAGG ACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAACGAAAAAAACAAACAAACAAAAGGTAAATAGTGAC CATGAAGGAAATCTGGGAAACTTAGAATGGCTCCTGCAGCCAGGCGTGGTGGCCACGCCTTTAATTCCAG CCCCCGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCAAGGCCATCCTGGTCTACAGAGTGAGTTTCAGGA CAGAGAAACCCTGTCTCAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178403
• Insert Size: 1983 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008544, AAH08544
• RefSeq Size: 3137 bp
• RefSeq ORF: 1983 bp
• Locus ID: 78697
• UniProt ID: Q91VU7
• Cytogenetics: 5 A3
• Gene Summary: Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which lacks an internal segment and is shorter, compared to isoform 1.