Fzd1 (NM_021457) Mouse Untagged Clone

CAT#: MC203642

Fzd1 (untagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fzd1
• Synonyms: AW227548; FZ-1; Fz1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053010
  GCGGCGGAGAAGGAGGAGGCGGAGGCGCAGGGGGGACCCAGGGCGGCTAGGCTGCCGAGAGTTGGGCGCT CCTCTGCCCGGCTGCGACCACTAACGCGGCGCCGCCTGACAAGAGCCACAGCCTGCCGCGGGCCAGGGAA GAGGGACGCGACCCCGGCGCGCCTGGACCACTCTTGCTCTCCTCGCCGCCCGCGCTTCATGAACCGCAAG TTTCCGCGGCGGCGGCGGCGGCTGCGGGACGCGGAGCAGAATCCCGGGGAGCGGGCAGAGAGCGGCCGCA CCCCGACGGAGGGCACGGGCGACCAGCTCTCCCGGATCACAGTGGGAGCCACCACCCGCCGCGCCGCTGC CCGTCCCAGTCAATCCGCGCCGAGGAGGGAGCCGAAAAAGTATGGCTGAGGAGGCGGCGCCTAGCGAGTC CCGGGCCGCCGGCCGGCTGAGCTTGGAACTTTGTGCCGAAGCACTCCCGGGCCGGCGGGAGGAGGTGGGG CACGAGGACACGGCCAGCCACCGCCGCCCCCGGGCTGATCCCCGGCGTTGGGCTAGCGGGCTGCTGCTGC TGCTTTGGTTGCTGGAGGCTCCTCTGCTTTTGGGGGTCCGAGCGCAGGCGGCGGGCCAGGTATCCGGGCC GGGCCAGCAAGCCCCGCCGCCGCCCCAGCCCCAGCAGAGCGGGCAGCAGTACAACGGCGAACGGGGCATC TCCATCCCGGACCACGGCTACTGCCAGCCCATCTCCATCCCGCTGTGCACGGACATCGCGTACAACCAGA CCATCATGCCCAACCTGCTGGGCCACACGAATCAGGAGGACGCCGGTCTGGAGGTGCACCAGTTCTACCC TCTGGTGAAGGTGCAGTGCTCCGCCGAGCTCAAGTTCTTCCTGTGCTCCATGTACGCGCCTGTGTGCACC GTACTGGAGCAGGCGCTACCGCCCTGCCGCTCCCTGTGCGAGCGCGCACGCCAGGGCTGCGAGGCGCTCA TGAACAAGTTCGGCTTCCAGTGGCCAGACACACTCAAGTGCGAGAAGTTCCCGGTGCACGGCGCAGGAGA GCTGTGCGTGGGCCAGAACACGTCCGACAAAGGCACCCCAACTCCCTCCTTGCTACCAGAGTTCTGGACC AGTAATCCGCAGCACGGCGGCGGTGGTTACCGCGGCGGCTACCCGGGGGGTGCCGGGACGGTGGAGCGGG GAAAGTTCTCCTGCCCGCGCGCCCTCAGGGTGCCCTCCTACCTCAACTACCACTTTCTGGGGGAGAAGGA CTGCGGCGCACCCTGCGAACCCACCAAGGTTTACGGGCTCATGTACTTCGGGCCAGAGGAGCTGCGCTTC TCGCGCACCTGGATAGGCATCTGGTCCGTGCTGTGCTGCGCCTCCACGCTCTTCACGGTGCTCACGTACC TAGTGGACATGCGGCGCTTCAGCTACCCGGAACGGCCCATCATTTTCCTGTCCGGCTGTTACACAGCGGT GGCGGTGGCCTACATCGCTGGCTTTCTGTTGGAGGACCGGGTGGTGTGCAACGACAAGTTTGCAGAGGAC GGGGCGCGCACGGTGGCGCAGGGCACTAAGAAAGAAGGCTGCACTATACTCTTTATGATGCTCTACTTCT TCAGCATGGCCAGCTCCATCTGGTGGGTGATCCTGTCCCTCACCTGGTTCCTGGCAGCCGGCATGAAGTG GGGCCACGAAGCCATCGAGGCCAACTCACAGTATTTCCATTTAGCCGCCTGGGCTGTGCCAGCCATCAAA ACTATAACCATCTTGGCGTTGGGCCAGGTGGATGGCGACGTACTGAGCGGAGTGTGTTTTGTGGGGCTCA ACAACGTGGACGCACTGCGTGGCTTTGTGCTGGCGCCTCTCTTCGTTTATCTGTTCATTGGCACTTCTTT CCTGCTGGCCGGTTTCGTGTCACTCTTCCGCATCCGCACCATCATGAAGCATGACGGCACCAAGACAGAG AAGCTGGAGAAGCTCATGGTGCGCATCGGAGTCTTCAGTGTCCTCTACACTGTGCCGGCCACCATCGTCA TCGCCTGCTACTTCTATGAACAGGCCTTTCGGGACCAGTGGGAGCGCAGCTGGGTGGCCCAGAGCTGCAA GAGTTATGCCATCCCTTGCCCTCACCTCCAGGGAGGTGGAGGAGTCCCACCACACCCGCCCATGAGCCCA GACTTTACAGTCTTCATGATCAAGTATCTCATGACGCTGATTGTGGGCATCACATCGGGCTTCTGGATCT GGTCCGGCAAGACACTGAATTCCTGGAGGAAGTTCTACACGAGGCTTACCAACAGCAAACAGGGGGAGAC TACCGTCTGAAACCCAGACTCTTACCTTACCCTTTTTTGGCCTGATCCTAGCCATCGCCTGGAAGCTGGC CCCAAGGAATTCCTGCCAAGCCTGGCCACTCCAGGCTTCCTCGCCAGCCACTGACTTTTGCAGGCCCCTT TCCCAACAAACAGCACAGGTTCTGCAAAAGCTTCCGTCCCTGGGGTAAAGGAACGAGAGGGCCCAACTGA TCGAGGTGTATGTGTATGTGCGTGTGGACCGATCTCTCTAGCCCTCTCTGATACCAGGATTGTACGCTTT TATGATTGTAAATAGCCTGTGTAAGATTTTTGTACGTATATTTGTATTTAAAGACTATCGAACACGCAGT TTTTTTTTCTTTTTAAAAATGTTTAATTATTTAGGGCAATTTACGTATTTGGAGCTTTTCTCACTTGCCC TTTTCTGCAGATTGCAGAGGAGGTAAAACAGAGCACACTTGACTAAGTGCTTTGCCCTGTGCCCTCATCT TTGTCAAGGGAGCATGGGCCTGCCTCTCCAGGGTCATTTCCTTCAGCATCTCCCGCCCCTCCCCTTCCTT GCTTGCAGTGGGAAATTTTAAGGTGCAGAACTCTGTAAAGCTTCCAGATCCCGAGGTGGGCCCCGCCCAT TCCAGATCCTCCCCTTTTCAGCGGTAGAGTGTGGATGGCTGTCCCTGTGGCTTCCTGATGCTGCTTATTT GAGATCCACCTTCCAGCTTTATTAGAGGACCCCAGTATGGGCTCTGCCAGTTGACCCAGCCTCCCTGTAC CCCGGTGTCCCTTGCCCAGTGTCTTTCTCCTTGCACCTAAGTTGTTCAGAGGGAGTGCAGTAACCGACCG ATAGCGAACTTTGGAGAGTCTGACTTTTTATGGATGAGCTCATTTTTACTCTCTAGTATCTGGAACTTGC TCTGGGTCTGGTCATCATCATGGAAAGAGCAGCAAGCTTTGTGGGTTGGGAGAGGCTAAAGAGAGAGGTT ATAAAACATTAGTTCTAATTGCATTCTGATGTCTGGCAACCTACTTTCTTCCCCCTGTGATGCTGCCTGC TTCTAGATTTTACCCTTCTATGAAATGCAGACCTCGAGGTTGCCTGGGAAGCTTGGTGAAGGAATGGTTT ACCTTCCTTAACAGGATAGTAGAACATACACAGAGTATGGAAACCTAAGGAGATTTCAGTGAAGTGGAGT TACTCCAAGTGAAACGCTGTTTTCTGGTTTTAGCCAAACTGCAATTAGATGTGTCTTTGCAATCTAAAAG TTGTACACAATCAACAATACACTTTGCTTACTCCTCAGCAGCACATTCTGAGGGAGAAACAGTCACACCC CCGCAGAAAGCCTGGTTGGACTTCCGAAGACAGAGGCGGTGGACTGACGGATGGGTGAGAGAGACAAACA CGACCTAGGCATGCATGCTGAAGGGGAAGTGTGTCCATTCCTACTGCATCCCATCTTGTGTGCTCCTGTC TGGATTTACGGCAGTGTGTCAAATGTAAATCTCTCAAAGCCATTTAAAAATACTCACTTTAGTTATGGAT GAAGAAGAGGAGAAAAGCAGTCCTCCTGATTGTAGTGTTTAAACCTTAAGAGTTTATCACAAATGCCGAT ACATACATAGGACCTAAATTTATCTATGTCTGTCATACCCTAAAATGACATTGGTTTTTGGATTTGGTAT GCATTATTATTGTTATTATTGTTATTATTATTCTCACCACCATGAGATCATCTATATTTATAGAGGAATA GAAGTTTATATATATAAAATGCCATATTTTTAATTTCGCAAATAAAAAAATGAAAGTTTTGTACAAAATT ACAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_021457
• Insert Size: 1929 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053010, AAH53010
• RefSeq Size: 4150 bp
• RefSeq ORF: 1929 bp
• Locus ID: 14362
• UniProt ID: O70421
• Cytogenetics: 5 2.61 cM
• Gene Summary: Receptor for Wnt proteins (PubMed:15923619). Activated by WNT7B (PubMed:15923619). Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (By similarity). Contradictory results showing activation by WNT7B have been described for mouse (PubMed:15923619). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:15923619). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).[UniProtKB/Swiss-Prot Function]