Gtf2i (NM_001080746) Mouse Untagged Clone

CAT#: MC203628

Gtf2i (untagged) - Mouse general transcription factor II I (Gtf2i), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2i
• Synonyms: 6030441I21Rik; BAP-135; Diws1t; GtfII-I; Spin; TFII-I; WBSCR6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053044
  GGAAAAAAAAGGAGGAGGAGGAGGGTGAGAGAGAAGCTGGGAGAGCAGAGAAAAGGGGGCACCGGCGGCC CCCCCGCTTCCCTGCACGCGCTTGCCGCCCGCCTGCCGCGGTTATCCCTGGGCTTCGTCCCTGTTCCCCC CCCCCCCCCCGGCGTCTGGCGACCCAGCGTGGAGCGAGGCCCCGCCGGGGGCGGCCATGCGGCGGTGACA GGAGCACGGCCGAGACGCACGGGCCCCCCTCGCCCTCTCACCTCCACCCCGTTCGCCAGCTCGCCGAGGC CCCTCCGGCCTACACTCGCTTGCTGTCCGCATCCCCCCCTCCCCGACCCTCCCCGGCTGCTCTTCGGGAA TCATGGCCCAAGTAGTGATGTCTGCCTTGCCTGCCGAAGATGAAGAGTCTTCAGAGAGCAGGATGGTGGT GACCTTCCTCATGTCTGCCCTGGAGTCCATGTGTAAAGAGCTGGCCAAGTCCAAGGCTGAGGTGGCCTGC ATTGCTGTGTATGAGACCGACGTGTTTGTTGTTGGAACCGAAAGAGGGCGTGCTTTTGTCAATACCAGAA AAGATTTCCAAAAAGACTTTGTGAAGTATTGTGTTGAAGAAGAAGAAAAGGCCGCCGAGATGCATAAGAT GAAATCTACCACCCAGGCCAATCGGATGAGTGTGGATGCTGTAGAAATTGAAACACTCAGAAAAACGGTG GAGGACTATTTCTGTTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTCCCTGTGCCATATGAGA AGATGCTGCGCGACCAGTCGGCTGTGGTGGTGCAGGGGCTTCCCGAGGGGGTTGCCTTCAAGCACCCCGA ACATTACGACCTCGCAACCTTGAAGTGGATTTTGGAGAACAAGGCAGGGATTTCCTTCATCATTAAGAGA CCTTTCCTCGAGCCGAAGAAACACCTCGGTGGTCGAGTGCTGGCGGCCGAGGCTGAGAGGTCCATGCTGT CTCCTAGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCTCTGGA AATGGCCGCTGTGACAGTGAAGGAGGAGTCAGAAGACCCTGATTACTATCAGTATAACATTCAAGGCCCT TCTGAAACTGATGGTGTTGATGAAAAGCTCCCCCTTTCGAAGGCTTTGCAAGGAAGCCATCACTCCTCAG AAGGCAACGAGGGAACGGAAGTGGAAGTGCCAGCAGAAGATTCTACTCAACATGTCCCTTCAGAAACCAG TGAGGACCCCGAAGTTGAGGTGACCATTGAAGATGATGACTATTCTCCACCCACCAAGAGGCTAAAGAGC ACGGAGCCGCCCCCGCCCCCGCCCGTCCCGGAGCCCGCCAACGCTGGCAAGCGCAAAGTGAGGGAGTTCA ACTTTGAGAAATGGAACGCACGCATCACTGACCTACGGAAACAAGTTGAAGAGTTGTTCGAAAGAAAATA TGCTCAAGCCATCAAAGCCAAAGGCCCGGTGACGATCCCGTACCCTCTTTTCCAGTCCCATGTTGAAGAT CTTTACGTAGAAGGGCTTCCTGAAGGGATTCCTTTTAGAAGGCCGTCGACGTACGGCATTCCTCGCCTTG AGAGGATATTGCTGGCGAAGGAAAGGATCCGCTTCGTGATTAAGAAACATGAGCTTCTGAACTCAACACG AGAAGATTTACAGCTTGATAAACCAGCCTCAGGAGTGAAAGAAGAGTGGTACGCTCGCATCACCAAACTG AGGAAGATGGTTGACCAGCTCTTCTGCAAGAAATTTGCCGAGGCCTTGGGAAGCACGGAAGCCAAGGCTG TACCGTACCAAAAATTTGAGGCCCATCCCAATGATCTCTATGTGGAAGGACTTCCAGAAAACATTCCTTT CCGAAGCCCCTCGTGGTATGGAATCCCAAGACTGGAAAAAATCATCCAAGTGGGCAATCGAATTAAATTT GTTATCAAGAGACCAGAGCTGCTCACTCACAGCACAACTGAAGTGACTCAGCCACGGACAAACACACCAG TCAAAGAAGATTGGAATGTCAGAATCACCAAGCTCCGAAAGCAAGTGGAAGAGATCTTTAATTTGAAGTT TGCTCAGGCTCTGGGCCTCACAGAGGCTGTGAAGGTGCCATATCCTGTGTTCGAATCCAACCCGGAGTTT CTGTATGTGGAAGGGCTCCCCGAAGGGATCCCCTTCCGAAGCCCTACCTGGTTTGGGATTCCACGCCTCG AGAGGATTGTCCGTGGCAGCAATAAGATCAAGTTTGTTGTTAAAAAGCCTGAGCTAGTTGTGTCCTATTT GCCTCCTGGGATGGCTAGCAAAATCAACACTAAAGCATTGCAGTCCCCAAAACGACCACGAAGCCCTGGG AGCAACTCCAAGGTTCCTGAAATTGAGGTCACTGTGGAAGGCCCCAACAACAGCAGTCCTCAGACCTCTG CGGTTCGAACTCCTACCCAGACCAATGGTTCAAATGTTCCCTTCAAGCCTCGAGGGAGAGAGTTTTCCTT TGAGGCCTGGAATGCTAAGATCACAGACCTGAAGCAGAAAGTGGAGAACCTCTTCAATGAAAAGTGCGGG GAAGCGCTTGGCCTTAAGCAGGCTGTGAAAGTCCCGTTTGCGTTGTTCGAGTCCTTCCCAGAAGACTTCT ATGTGGAAGGCTTGCCCGAGGGGGTGCCCTTCCGAAGGCCGTCCACATTTGGCATTCCCAGGCTGGAGAA GATACTCCGGAACAAGGCCAAGATAAAGTTCATCATTAAAAAACCTGAGATGTTCGAGACGGCCATCAAA GAGAGCACCTCTTCCAAAAGCCCTCCAAGAAAGATAAACTCATCACCCAACGTTAATACTACTGCATCAG GTGTGGAAGACCTGAACATCATCCAGGTGACAATTCCAGATGACGATAATGAACGACTGTCCAAAGTTGA AAAGGCCAGGCAGCTGCGGGAGCAGGTCAACGACCTCTTCAGTCGGAAGTTTGGTGAAGCTATTGGGATG GGCTTCCCGGTGAAGGTCCCCTACAGGAAGATCACCATCAACCCTGGCTGCGTGGTGGTCGATGGCATGC CCCCCGGGGTGTCCTTCAAAGCCCCCAGTTACCTGGAGATCAGCTCCATGAGGAGGATCTTAGACTCTGC GGAGTTTATCAAGTTCACTGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGCTGGTTGATCAG AACGAGTCTGAAGGCCCTGTGATACAAGAATCAGCCGAGGCAAGCCAGTTGGAAGTCCCAGTAACAGAAG AAATTAAAGAGACAGATGGGAGTTCACAGATCAAGCAGGAGCCAGACCCCACGTGGTAGACCGTGGCTCT CCAGGTTCGTCCAGTGTCCAGCGTGCGTGGTGGAGAAGAGCTTTGGGACCCGTTCCTGCCAGCTCTGTAA TATACTTGTATAACAGAAATACCTTCTATACAAACCTTCTTTTCTACTTTTAGATAGAAATGTCTACTTT CTCAGCAGTTCTGTGAATTGAGCTACACAGCGTGATGAGATGCGCGTGCCTGGTGGCTTGGGAACGCACT AGAAGGGTCTTGCAGCAGTGCTGAAAGAGGCGGGAATGGCAGGGACGACTCGTAGGGTTTCTTATGCATC GGGCAGAGCCTTGGGCTGTGGTGCGTGTTCCCGTCAGTGAGATATTCCTACAGGGCATCTTGGCTTCCGC AGCAAAGGAAGTCCCTGTGCTAGTCAGTGCTTTAGTTTTTCTATTGAAGACCAGTTCCGTGGTCTTGCAC TCGGCTGCCCCTCCTGCCAGGGGAGGGCTGCTGTCTGTGTGTGTGACTAAGCCTCTTCCCAAGCTCCATC ACGATTCCTTTGTCCTCACTGCCATTCAGAGCAAAGCCGGCCAGCTCGCCTGACCGCCAGTGAGCAGCCA GTGCATCCGTGCAGTGCAGCTGGAGAGGCTAGCCTCAGAGCCTCGCTCCGGTTACTTTACAGCAAGTAGA TTTCTGGGTGTGTCCTAAAGGTCAGAGGTCAGGTCTGCATGCTTGAGCAGGTGGAGGATTTGCCCCTCCC CAACTGGGACCCACAGTGGCCTCAGGGGCTCTGGGCCGCAGCTCTGGGTCTGGGTCGAGTCAGGCTGGGC TGTGTGTCTGCCTGGTTGGTTAGTCTCTAACCACCTGTCTGGCCTCTGCGCCAGGGTGGGGAGTGAGGAC ATTGGTGTCCCGCCCTATGTGGGGGCTCACAGACGATGATAAGGTAACAGCTCTCAGCAGTTAGGCACGT GAGGGCACGGTTCTCGGTAGTCACCACAATGTTCACAATGGGAAATTATATCACACGGTAGCAGAGATAG GCATTTTATGTGTTCCTTATATTTTACCTCAAATTGTAGATATAGGGTAATAAATAAAATCCATCCATGC CTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_001080746
• Insert Size: 2997 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053044, AAH53044
• RefSeq Size: 4461 bp
• RefSeq ORF: 2997 bp
• Locus ID: 14886
• UniProt ID: Q9ESZ8
• Cytogenetics: 5 74.48 cM
• Gene Summary: Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as Gamma).