Marchf8 (NM_027920) Mouse Untagged Clone

CAT#: MC203594

41341 (untagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Marchf8
• Synonyms: 1300017E09Rik; M; Marc; MARCH-VIII; March8; Mir
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053090
  CGCGCCCACCTGAGCTGCTCGCGCCGAGGTGCGGCGTGGCGGGGCCGCGCGCTCCGGGCCACCAGGGGTG TGGCCGCTGGGAGGCCGCGACAGGTGGGTGCGCGGAGGTCTCGGGCCGGGGAGAGGCCGCGTGCTGCGGG GCGGGGTCCGGTCACCCTATCGGCGGGCGGCCGAAGGTCTCGGGCTCTCTCAGGTGAAGTCCTGCCCGGG CTGGGCTGTGAGGGCGCGCACCGGGCAACCCTCCCTCCCTGCCTCTGCTTCAGTCTCCGCGTCCGTCCGA GATGGAAGTGTGGATTCCTGGTGTCTGAGTGCCTGACGTGGCGTCTGGACGGTGCTGCTCTAGTAGAAGC GCTGGAGGCAAAGCGTAGCTTGAAGTCAATGGCCCAAAATGACTCTGCTAGAGGAGACTCACTTCCGTAA GGAGGAAGACCAGGTCGGAGGCTGGGATGAGCATGCCATTGCACCAGATCTCTGCCATCCCTTCTCAGGA TGCCATTTCTGCTAGAGTCTACAGAAGCAAGACCAAAGATAAGGAGCAGAATGAGAAGACTTTGGGACAT TCCATGAGTCATCCAAGCAACATTTCTAAGGCTGGGAGTAGTCCTCCATCCACGACGGCTCCAGTGTCTG CCTTCTCTCGCACTTCTGTCACACCATCCAACCAGGACATCTGCAGGATCTGCCACTGTGAAGGGGATGA CGAGAGCCCTCTGATCACCCCCTGTCACTGCACAGGGAGCCTCCATTTCGTGCATCAGGCTTGCCTGCAG CAGTGGATCAAGAGTTCTGACACACGCTGCTGTGAACTCTGCAAGTACGAGTTCATCATGGAGACCAAGC TGAAACCTTTGAGGAAATGGGAGAAGTTGCAGATGACTGCCAGTGAGCGCAGGAAGATCATGTGCTCAGT GACCTTCCATGTCATTGCTATCACCTGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGCACAGCAGAG GAAATCAAGCAGGGTCAGGTAACAGGAATCCTAGAGTGGCCTTTCTGGACGAAGCTGGTAGTTGTGGCCA TCGGCTTCACTGGAGGACTTCTCTTTATGTATGTTCAGTGCAAGGTGTACCTACAGTTATGGAAAAGACT CAAGGCTTACAATAGAGTGATCTATGTTCAGAACTGTCCAGAAACAAGTAAAAAGAATATTTTTGAAAAG TCTGCACTTACAGAGCCCACCCTTGAAAATAAAGAAGGACATGGAATGTGTCATTCCACCACAAATTCTT CTTGCACAGAGCCTGAAGACACTGGAGCAGAAATTATTAACGTCTGACCATGTGAGGGTGTCATTTCCTT GATGTCCACCAACAGCTGAAGGAATTTGTCTGCTGCCAGTTGTGTTCCTTCTTTCTGTCGTTTAATAGCA TAGACTGGGCAGGTGACTATTTATAGTGGCCTCTCTTTTTCTCAACCCTCCTTGGTGTGAATGTCCTAGA ATTCTTGTGGGGCAGGTACCACTGGGTTCCATTTTGCCAGGCTTCTCTGTGGTCTGGGAAGGTGGGAGAC CCCATGTATCCATGACAAGGAGCTGGCTAAGTTCCAGCCCCTGGTCTTGAATAGATTATGGAAGTAAAAA GCTAAAGCAGAAGACAGAGCAGCATGGGGAGAGGAGGCGGGTGATGGAGGGTCCCCAGAGGACAGAGCAG CATGGTGCGGGAGGGCTGCTGCTGCTGGGAGGGGTCCCTCACACTAACGAGAGCTAGGAGTGGCAAGGGA GCAGTGCCTCCAGACAGCAGCCCTGCAGCCCCTGCCTTGCTCAGCTGATGCCACCACCTTGCTCGGGGGG CAGACAAGGCCTTGGGCTGTGTCTGCTGGTGTGTGACTCACTGAGAAAACAGCACTTTATGAGAGTTTTT ATTTTGGAATTCCTGTGTTTAATGGGAGTGACATGTTTAAACACGTGTCACATTTAGAAATGTTCTCTTT ATTGCCTGTTATCTCTTTGCATCAAAATCGAACTGAAACCATTTCTGCCTTATATATTCTGAGTAGTAGA TGTCCTTCCATGTCCAGAAAAGACTTCAGACTTTAAGCAAGACAGACAGGTCTGAGACTCCATGGGAATT AATGGATGCTAAGAGTGTAATTCCTCTGGTTTTGAGCCTGTTTCCTGAAAAGTCCTATCTCACAAGCTCA CCCATACATTTCTTTGTTGTGAGCGTTGAAATGAAAGGGTTTTGAGATGTAATGAATCTGAATCAGGGTG AGCATTTAAAAAACGCTCCTTTGACTGACAGATGTCTCCAGATGGAGTGGGTACACATTGGGGACACTCC TGTGATCCACCTCTGCTGTCCCCTGTGCTGACAAAGTTCTGTGGAGTCATTGCTGAGGGCTGGGAGGTCA ACAGACCAGCTCTGGGATGAGGTCTTGGGTGAGCAGTCAGCTAATTGCATCGCCATTGTGTCACTGACCT GGCCTCATACCCACTTATTGCCTTAAGCAGACTTGACAGGATCACTAGGGTCCTTAGCTCCAATGTGACC TATTCTGTCTTGGGGTTACCTCCTGCGCGATATTTTCACAAGACAGACAAAATCAAATCACTACTGCTAA GTGGCCATTGCCCCTTTAAAAAGTTTGTTGAACATTTTTTTTCTTGAAGGCCATTGTCAAAGTGCCAGGC AATAGTCAACTTACAATGTGACAAGAGGAACCGCAGAAGTGTACTACTCTCCAAGTGGCTGCTGTCGGCT CCAGGGTCTTCAGATTCTCTTGAGGTGGCCAGGGCAATGCCTAGGGTCCATCTCCCTGAGCTTTGGCTGC TTCACCTGTCTTCTCCAGAGACTGAGGCAAACCGAAGATGAATCTTTTGTAGTTCCTAGTAGAAGCCCCG TGCTTAGTTTTCAGAAGCATGTGGAGGCTAGCTAGAAGGCTTGTGGAAGCAAGTTCTGTCCTGTTATCCC CTTGTCTACTCCCTGGAGTCATGGAGTTCAGTTGTGGAGCTGTTCTGCCAGGGCTTTCAGCCATTATCTA ATGGGCCCACCCCTTAGGTCAGCCTGCCCTCTCTCTCCCAGACCACATAAGCCTCCCTGTGCTCACTGTG CCCCAAAGTGCAATTACTCACACTCAGTGAGGACACCAGGCAGCTACAGACTGCCCACTAAGCTCTGACT ATTCCTTTTAGCAGCTGCTGCGGCGAGTCTCTGACCCTGTCCATCTGTCTGTGACAAGATGTTCCAACAT TGGGTGGGCAGTCAAATGAAGATGGAGGCATTTGTAGAGAAGAAAGTGTCCCTCAATGGGGGTTGTCTCT GCACAGCTGCCCTAGGGGACTTGTACTCCCACATAGATAGGTGGCTTTGGAAGCATGGCTTCATGTTGTT CCCTTTATTTCAGGAAGGCCTGATGCTATCTCTTCTCTGGCTTCTCTGATGAACTCTGTCCTTCATCCTT CCCCCGCTCGTTTTCCCTGAAGGTTCTCTGATCTAGTAAACAACTACACATGTGACTCAGTGCAGGAGAC TGGCAAGGCACGGCTGCGAAGCATCTGGCAGAGCTCTGGGTCTGCCCAGACTCTCCTAGTCCTTGCCTCT TTGGCCTCTGAGTAATTTTGCAGGGCTATCTGTCTACCCCATTGTTCCTCACTTTGAGCTTAGATATAGT GGGAAGATGGCATGTAGCTAGACCAAGCAGATCTAGGATGAGGGAGACCTCAGGCCAGGAGGGCTTGTGC TTGTGGGTCAAATGAAGAAGGAAGTCGTCTCAGGGTGTCACTGGCAGCCCTCTAACTTGGCCTTTACCCC GTATGTCATAGACCCATTCGCACAGCTTTGACTCTAGCTGCCACAAGTGAGTAGTTGAGGGTTATGCTCA AGTGTATCACAAGATTTTCCTGGAAATGTAAACATTCTCTTAGTCTAGGTCTCTCTGTCTCTCCTTTCTG GCTTCAGGACACTCTTCGTTAAGTAGGCTTAGTTTTCAAACCTGTGGGTATGTCCAAGTGGACACAGATG TGCAGGCTCAGGCGGTGGGGGATAGGACAGTTCAAGGCCTGGGGTAGTATCTAAGTGACAGAGGGGAAAG GCTGTGTGGGCAGGGTGAAGAGCTGTAATCTTGTGGGTGTGGTTAGATGCACATGTATATATTTCATTCT ACCTGTTCAAATCTGGTTTGGAATAATGCAGCTTATAGCTGCAGCTCAAGGAAAGCCCAAAGCATAGGGA GAGAAAAGGCACTTGTCTCTGTGTCGGGAGTGAGTGACGAAGGCATGTAACTGGTGCACATTTAGATGCA ACGTTTCAAGTTGATTGTTAATAAAAACCAAATTTACACTGGTGTGTGTAGTGTAGTTTCTAAAAAGCAC TTCACATTTTAAATTTTTCTTGGAAAATTTCTAGTAATCTAAATGTCTAATTTTTTAATCTTTTTGTGTA TGTTCATTGTTTCTCAGTATTACTGCTTGAATAATTCTCTGTACAGGGGATTTGTTTGTGCTATATACGG GGTGTCTAAAGTTAGCAATAAAGCCTTTCTTTACAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
• Restriction Sites: AscI-NotI
• ACCN: NM_027920
• Insert Size: 861 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053090, AAH53090
• RefSeq Size: 4553 bp
• RefSeq ORF: 861 bp
• Locus ID: 71779
• UniProt ID: Q9DBD2
• Cytogenetics: 6 E3
• Gene Summary: The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2 and 3 encode the same protein (isoform 1).