Gtf2e1 (NM_028812) Mouse Untagged Clone

CAT#: MC203488

Gtf2e1 (untagged) - Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2e1
• Synonyms: 56kDa; 2610024P03Rik; AI303800; FE; TFIIE-A
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC056966
  CGGATTAGCGGGCGGTTTCCAAACCGGTCGCAGGAATTAGGCGTTCGTCCTTGTCTCGCAAGTGGCGTGG GCTGGCTGAAGCCGGTAGTTGAGGTGCTGAGGGCGGCTGTAGTGGGAGTGTTCGAGCATCCACTTGGTTG AAGGTGAAGCTTCCTGTTGAAGATGGCAGACCCAGATGTTCTCACTGAAGTTCCGGCGGCATTGAAGCGG CTAGCCAAGTATGTCATCCGGGGCTTTTATGGCATTGAGCACGTGTTGGCCTTGGACATCTTGATCCGGA ACCCCTGTGTAAAAGAAGAGGACATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGATCCGTTTT GAATAATTTAAAGGGAGACAAGTTCATCAAATGCAGAATGAGGGTAGAGACTGCTGCAGATGGGAAGACC ACCCGCCATAACTACTACTTCATCAATTACCGCACTCTTGTTAATGTAGTGAAATATAAGCTGGACCACA TGAGAAGAAGGATTGAAACAGATGAGAGAAATTCCACCAATCGGGCTTCCTTCAAATGTCCTGTCTGCTG CAGCACTTTCACTGACCTAGAAGCCAATCAACTGTTTGATCCCATGACAGGAACTTTCCGCTGCACCTTT TGCCATACAGAGGTAGAAGAAGATGAATCAGCAATGCCCAAAAAAGATGCTCGCACGCTTTTGGCAAGAT TTAATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTAAACTTGGCCTATGAAAT ACTTGAACCAGAACCCACAGAAATCCCAGCACTGAAGCAAAGCAAGGACCGAGCAGCAACTGCTGCTGGA GCTGCTGGCCTGGCAGGTGGGCACCACCGAGAGGCGTGGACCAACAAAGGCCCCTCGTATGAGGACTTGT ATACTCAGAATGTTGTCATTAACATGGATGATCAAGATGATGTGCATCGCCCCTCGCTGGAAGGGAAAGC TGCCAAAGAGAGACCCATTTGGTTGAGAGAGAGCACTGTCCAAGGGGCATACAGTTCTGAAGAGATGAAG GAAGGTGGCATCGATGTGGACACATTTCAGGAGCGCGAGGAAGCCCGTGCGGGGCCTGATGACAATGAGG AGGTCATGCGAGCTCTACTCATTCATGAGAAGAAGACTTCCTCGGTGACAGCTGGCTCTGTGGGAGCAGC CGCTCCGGTGACAGCTGCTAATGGCAGTGACTCTGAGAGTGAGACCAGCGAGTCAGATGATGACTCTCCA CCTCGGCCAGCAGCTGCGGCTCCTCCTCATCATCATCGCGATGAAGATGAGGAAGACGAGGAGTTTGAGG AAGTAGCAGATGACCCCATTGTTATGGTGGCTGGCTGTCCATTTTCCTACAGTGAAGTTAGCCAGCGGCC GGAGCTGGTGGCCCAGATGACCCCAGAAGAGAAGGAAGCATACATAGCCATGGGCCAGCGTATGTTTGAG GATCTCTTTGAGTGAGCTTTCCCTGCTCTCCCTGCTGCTGCCCATTGCAGAGAAACTCCCATCAGGTGCT TAAGGGCCTTCCCGCCCGTGTAAGAAAGCACCTGGTAATCTAGCACAGAAGCATAGTAGGAGAGCAAATT TGATTTTTTTTTTTAATGTAAGAAACTTCCCAGAAAGGTAGGTAGGTTATAAGTATTCAGTTCCCTCTTA CTATTAAAGTATTAATATTTTTCTCTATTTCTTTATCTAATTTTTTCCTTTCCTTTTTAAGACATAGTTT TCTCACTCTGAGTAAAATGTTCCTGTCTACGTTCAGAACCCCACTAGGGATAAGTGTTCCCTAGTATTGC AGCTGCACTGTGGTCAGGCGTCTGAGACAATTGCTTATAATCCTGCCAACCAAAGCAGCTTACCAAGAAG CAGGTCACTGCTTAGGGGAATGGAGTACCCAAGCACTTCATCTTCCTCAAAACTGAGTGTGGGCCAGTCT GTAAATGGAGTGAGAGAGTACAGGAGAGGGGGGCCTGAACCTTAGGTTCCTGTGTAAGCTAGAACCCTCG TCTGTAGAGCCCATAAGGCCGGTAGATTGTTTCCTTCTCATAGACCTTTTGGGTTGTACTTTAGATAAAC AAAGCAGGTTAGGTCTACCCGCATGGAGAAATTCAAGGAGCACATGCATGAAGGGAAGGAGTCTGCATCC AGGTTGACGAATGGAGAGTACTGGGAACTGTGCCTTAGAGCTGCCTCAGTGTGATTTGGAAATCTCAGAT GGAAAGCTGAGACTGGCCCCACTTCCCCCACTTGTTCTACAGACCTGTGCCAGCGCTCTGAATTGAGGAG CCTTTCCAGTACTGAGCAGTGTAGAAGTGCTGATTCCCCCACTTTCCTCACTCTTCCAGAGGTCATCAAA ACCTTTATTTGTCTCTCCTTTGAGCCTCATCAGATATCGTACTTCAGTGCCTCTACTGTGTTGTTGAGCA TTGTAACCTCAAGGAACATAGTCATTAGCATGGTGTTATTCCTGAAGGCAGAACTTAAAAAATAAAGGAC TTTCATATAAGGTCTACAGCAATGAAACTTTTATAGTATTTTCCCTTGCATTGTGACTTTTAGTTGTCAT TTTGTAGTATATGTGTGAAGCTAAGAACGTTTTAGAGAATCTGAGCCTTGTATAAAGTGATGACATGGTT ACCTGGTTGTTGGATGACTGAATATTGACAGTCGACTTACTACAGGGTAAAGCTGGTTAGTTTCTGGCTC TGACTTCTTGGAGCATACCTACCATGATTACTCTTTCCATGTAAAGATGTTAGAGGCTCCTCATCGGTCA TTGTCTTAAGAGCACAGGTGCAATTTGATGCGACTTAAACTTTTGGCAATGAATTGCCTTAAAAAACCTT TTTATGTATTTATCTTAAATACATGAACTGTTCAACTTTAAAATTTTGTTTTTTAAACCTTCATGGTTGT AGAAAGATGTTAATTTGTAAAACGTTAATTTTTAAACTATATGTTCTTTGTTTCGTTTTATATGAGATTG TGTAAGTTACATTTCAGTAGTGGAAAATGTACTAATAAAGGCGCTCATTAAAGCTGCATTTGAAATAGAA AAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_028812
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC056966, AAH56966
• RefSeq Size: 3096 bp
• RefSeq ORF: 1323 bp
• Locus ID: 74197
• UniProt ID: Q9D0D5
• Cytogenetics: 16 B3
• Gene Summary: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function]