Kdm2b (NM_013910) Mouse Untagged Clone

CAT#: MC203452

Kdm2b (untagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kdm2b
• Synonyms: Cxxc2; Fbl10; Fbxl1; Fbxl10; Jhdm1b; PCCX2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057622
  GGCGCGCGGTGGCGGCGGCGGCTCCCTCCTCGCCGCGCGGGAGCCCGAGCGCTAGCCAGGGTGAGCCGAG CCTGCTCCCTGCGGGCGGCGGCGGCGCTGCTCCAGCCATGTCAGCGCGCGCGAGGCTGGGCCCACCATGA GCCATGGCCATGTCCGTGAGCGCCGAGGACGACGACTATGAATCGGAGCCCGACCAGAACCGGGTGGTGG GTCGGCCTAAGGGCAAGTTGGGCCCGGCCTCAGCGGTGAAGTTGGCTGCCAACCGAACAACAGCAGGAGC TCGCAGGCGCCGGACGCGATGCCGCAAGTGCGAGGCCTGCCTGCGGACGGAGTGTGGAGAGTGCCACTTT TGCAAGGACATGAAGAAGTTTGGAGGTCCTGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCG CGCCAGTGCTGCCCCACACCGCCGTGTGCCTTGTGTGTGGCGAGGCAGGGAAGGAGGACACAGTGGAAGA GGAAGAAGGCAAGTTTAACCTCATGCTCATGGAATGCTCCATCTGCAACGAGATCATCCACCCTGGATGC CTTAAGATTAAGGAATCGGAGGGTGTGGTCAACGATGAGCTTCCCAACTGCTGGGAGTGTCCGAAGTGTA ACCATGCCGGCAAGACCGGGAAACAAAAGCGTGGCCCTGGCTTTAAGTATGCCTCCAACCTGCCTGGCTC CTTGCTCAAGGAGCAGAAGATGAACCGGGACAACAAGGAAGGGCAAGAGCCTGCCAAGCGGAGAAGTGAG TGTGAAGAGGCTCCCCGTCGGAGGTCAGACGAGCACCCCAAAAAGGTGCCTGCAGATGGCATCCTCCGCC GAAAGTCTGATGATGTGCACCTGAGGAGGAAGCGGAAATACGAGAAGCCCCAAGAGCTGAGTGGACGCAA GCGAGCCTCGTCGCTTCAAACGTCCCCCGGTTCCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGC AGTCTCAGCCCTTGGTGGAGATCCAGTCTCACTTACTTCCAGCAGCAGCTAAAACCTGGCAAAGAAGATA AGCTTTTCAGGAAAAAGCGGCGGTCCTGGAAGAACGCTGAGGATCGTCTGTCACTGGCCAACAAGCCCCT TCGGCGCTTTAAGCAGGAACCGGAGGACGATCTGCCTGAGGCACCTCCTAAGACCCGGGAGAGTGATCAG TCACGTTCCAGCTCACCCACTGCTGGTCCCAGCACTGAGGGAGCTGAAGGCCCAGAAGAGAAGAAAAAGG TGAAGATGCGCCGGAAGCGGCGACTTGTTAACAAGGAGCTGAGCAAAGAGCTAAGCAAGGAGCTCAACCA TGAGATCCAAAAGACGGAGAGCACCCTGGCTCACGAGAGCCAGCAGCCCATCAAGTCAGAGCCTGAGAGC GAGAACGACGAGCCCAAGAGGCCCTTAAGCCACTGCGAGCGCCCCCACCGCTTCAGCAAAGGGCTCAACG GCACACCTCGGGAGCTGCGGCACTCGCTGGGACCTGGCCTGCGTAGTCCACCTCGTGTTATGTCCCGGCC CCCGCCCTCTGCATCCCCACCCAAGTGCATCCAGATGGAGCGTCACGTGATCCGGCCACCGCCCATCAGC CCCCCACCTGACTCGCTGCCCCTGGATGATGGAGCAGCCCACGTCATGCATAGGGAGGTGTGGATGGCAG TCTTCAGCTACCTCAGCCACCGAGACCTGTGTGTCTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTG CTGCGATAAGCGGTTGTGGACCCGCATCGACCTGAACCGCTGCAAGTCCATCACACCCCTGATGCTGAGC GGTATCATCCGGCGACAGCCTGTCTCCCTTGACCTCAGTTGGACCAACATCTCCAAGAAGCAGCTGAGTT GGCTCATCAACCGGTTGCCTGGGCTCCGAGACTTGGTGCTGTCAGGCTGCTCATGGATCGCTGTCTCAGC CCTCTGTAGCTCCAGTTGTCCATTGCTCCGGACCCTGGATGTCCAGTGGGTAGAAGGACTAAAGGATGCC CAGATGAGGGATCTCCTGTCTCCACCCACAGACAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCC GGAACATTGTAGAGCTGCGCCTAGCTGGCCTGGACATCACAGATGTCTCCCTGCGGCTCATTATTCGCCA TATGCCCCTGCTCTCGAAGCTCCAACTCAGTTACTGTAACCACATCAATGACCAGTCCATCAACCTGCTC ACTGCCGTCGGCACCACCACCCGAGACTCGCTGACAGAGGTCAACCTATCAGACTGTAATAAGGTAACTG ACCTGTGCCTGTCCTTCTTCAAACGCTGTGGAAATATCTGTCATATTGACCTGAGGTACTGCAAGCAAGT CACCAAGGAAGGCTGTGAGCAATTCATAGCTGAAATGTCTGTGAGTGTCCAATTTGGGCAAGTGGAAGAG AAACTCCTGCAAAAACTAAGTTAGTTCCAGGACAAGTATGTAAATATGGAGGGTTGGGGGGAATACAACC AGGAAGGGTGAAAGACTGCAAAATCAAGTGGGCCTATTATTTCCCTTTGGAATTTGAAACAAGAGACTAC AGGACACAGTTCTGTTTTGCAAATCTTGCCAAGGGGGAAGCCATTCAACCACCCATTTTGGGATGAGTGA GCCAGATGTTTTCCTTTGCTCTTTCTGCATCGTAACTGCACTGCTTTCTGGACCATTTCTAAGGCGGCCT TTAGAAGACATTCCAATTGGAAAGGAAGATGAGCTTTGGAGAAATCCTCTTAGAAGCCTAAGCTTGGTGT TTTTATCAGTAGTTTTTGTTTGGGGCACTTGTTCCTTGCAATGCTGAGGCTTCTGGCGTCAACATGGAGT GGACCACACACACACCATCTTCAGCCGTCGTTGATACATTTTGTTTAGTTGGTTTTGTTACATCTTACAT TATGCAGAACTATTTTTGTATAAATTGTTTAAAAATTATTTATGGGAGATTTGAATGCATACCAGTGTTT TTATTATTTAGATTGCCAACTGTCCTTCAGGTAGGAGAGTTAACCTGTACTATGTCAGCATAAAAAGCCC ATCTCCAGATGTGCCCAGGTGTGACAGGGGGCTATGACCTTCCACTTAAAAAGCATGTTTAACTGGATGC TGAGAGCCTACTGTGTATGTCAAGAAGCTACACAAGCATGTTGTGGATATGTGTAAAGTATAGTCCAGGT AAAACACTTTGCCAAGTTTCAATAGACTTCACCTCTCCCTCAGACTTGAGCCTTTACACTCAGGAGGGTT TGCTCCAAGCATGAGCTCTTGTATTCTACTTACATAGGTCTAATTTATACCAAGAATCAGGTAGAATAAA TGTGCCCACACCCTCTCCTTTGTCCTCCTCCTCTCAAGTGTTCAGTTCTCACTTTAGTTGGTAAGTTCTG CATCCCTTGTTGTTTGGTGGTGTTTCCTAACCTTTCCCTGATGCAGTTTGACCTTTGTTGGATTTATATA AACAATTGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_013910
• Insert Size: 2331 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057622, AAH57622
• RefSeq Size: 3545 bp
• RefSeq ORF: 2331 bp
• Locus ID: 30841
• UniProt ID: Q6P1G2
• Cytogenetics: 5 F
• Gene Summary: The protein encoded by this gene is a H3K36-specific histone demethylase, which contains an N-terminal jumonji C domain, a CxxC zinc finger domain, a plant homeodomain finger, an F-box, and eight leucine-rich repeats. Amongst its demonstrated functions, this protein plays roles in the suppression of premature cellular senescence, leukemia maintenance and development, maintenance of mouse embryonic stem cell pluripotency, and induced pluripotent stem cell generation. Mice homozygous for a targeted deletion of the zinc finger domain display embryonic lethality with development ceasing at approximately 7 to 8 days post coitum, demonstrating an essential role in early development. A pseudogene of this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) lacks exons in the 5' UTR and 5' coding region and uses an alternate 5' terminal exon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.