Helq (NM_001081107) Mouse Untagged Clone

CAT#: MC203440

Helq (untagged) - Mouse helicase, POLQ-like (Helq), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Helq
• Synonyms: AU021725; D430018E21Rik; Hel308
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC082601
  CTGATGCTGGGTCACCTGCTGTCTCCAGAACGGCGCCACCGGCTCCGGGCTGCACCCAGGGCGGCTGTCG CAGGGACTCTGGGCCGTTGTCATGGAGGACGGCTGTCCCCGCATCCGCCGGCGGGTGTCTGTCCGCAAAA GGAACCGCGGCAACCTGGAGAACCTCCGTGCCTCCCCGACCCCGGCCGAGCTCCAGCCTGCCGAGGACAC GGAGGACGAAGCTGCAGCCGGGAGCCGCCGCCGGAAAACCGGGAGCCCGGAGCACGCCCAGGAAAATGAC AGCGAAGAGGACATGTTTGGTGACTATGACAGCTTTACAGAAAGTTCCTTCTTAGCTCACGTTGATGACC TGGAACAGAGATATATGCAACTGCCTGAATGTGGGGACCGTGATGCAGACTCTGGCACCAAAGACCTTTG TTCAGCAGGCCTCAAAAACAACCTCAGAGTTACTACTGTAATCAACCTCACTGACCCGGAAACTAGTGAG CACGGCCAGAAGCAGAGTCACCTAGATGTCCCTGCTGAACCAGAACCTGGAAGCGATCTTTCGTTTGATG TGCCTTCTTCTCAAATTTTATACTTTGAAAATCCGCAGAACTCACCAGAAGCTTTGGGCGATCCGTGCAC TAAGAAGACAAATGGGGACCCACAGAAGTCATCACATGAAGAGTTGGTCTCCAGCCACACAGAGCAACCC GAGCCAAATAATGACTTCTCTAACGTCAGAGCTGCTTCAGAGTCAAGCAGGAGAAAAAGTCTGAAAGACC ATCTGAAAAGTACCATGGCTGGGAATGCCAGGGCCCAGACCCCAGCATTTCCCAGGAGTAAACACCTCAG AGAGGCGCTCCTGTCTGAGGAGATCAGCGTCGCCAAGAAAGCCATTGAGTCACCGTCAGATGATCTTGGT CCTTTTTATTCGCTACCCAGCAAAGTGAGAGATCTTTACGTTCAATTGAAAGGAATCAAAAAGCTATATG ACTGGCAACATACTTGCTTAACGCTAAGATCTGTGCAAGAAAGAAAAAATTTAATATATTCCTTGCCAAC GAGTGGCGGGAAAACCCTTGTGGCTGAGATCTTAATGCTGCAAGAGCTGCTCTGCCGACAGAAAGATGTC TTAATGATCCTTCCGTATGTGGCCATTGTCCAAGAGAAGATTTCCAGTTTATCGAGTTTTGGTATAGAAC TTGGTTTCTTTGTTGAAGAATATGCTGGAAGCAAAGGAAGATTTCCTCCAATTAAAAGGAGGGAGAAGAA ATCACTGTATATCGCCACTATCGAGAAAGCGCATAGCCTGGTGAATGCCTTGATCGAAACCAGCAGGCTC AGCACTCTGGGTCTGGTTGTCGTCGATGAGTTGCACATGATTGGTGAAGGGAGCCGTGGCGCCATACTGG AGATGACCCTGGCAAAAGTCCTCTACACCAGCAAAACAACTCAGATTATCGGCATGAGTGCAACATTGAA CAACGTAGAAGACCTGCAGGCGTTCCTTAAAGCCGAGTACTACACCAGTCAGTTCAGACCAGTGGAATTA AAAGAATTCCTGAAAGTAAATGACACAATCTATGAGGTAGACAGCCAGGCTGCGGACGGCATGACCTTTT CACGGCTCCTGAGTTATAAGTACTCTGAGGCCCTGAAGAAGATGGATCCCGACCGCTTGGTAGCACTGGT GACAGAAGTTATTCCTAACTACTCCTGCCTGGTGTTTTGTCCCAGTAAGAAGAACTGTGAAAATGTAGCA GAAATGTTGTGCAAGTTTTTAAGCAAGGACTATCTAAACCACAGAGAGAAAGAAAAATGCGAAGTGATTA AAAGTTTGAGGAATATCGGCAATGGTAAAGTGTGTCCTGTCCTGAAGCGCACCGTCCCTTTCGGCATCGC CTATCACCACAGCGGTTTAACTAGCGAAGAGCGGAAGCTTCTGGAAGAGGCTTACTCCACAGGCGTGCTC TGTCTCTTGACCTGCACGTCCACCCTGGCAGCAGGGGTCAATCTGCCTGCACGGAGAGTCATCTTACGAT CTCCCTATGTGGCTAACACGTTCTTAAAGAGGAATCAGTACAAGCAGATGGTTGGCAGGGCCGGCCGCGC TGGGATAGACACTGCCGGGGAGAGCATCCTTCTGCTGCAAGAGAAGGACAAGCAGCAGGTCCTGGAACTC ATAAGCGGACCCCTGGAGACCTGCTGCAGCCATCTTGTCGAGGAATTCACCAAGGGGATCCAGGCTTTGT TTCTCTCTTTAATTGGTCTGAAGATCGCAGCAAGTCTTGGTGACATATATCAGTTTATGAGTGGTACATT TTTTGGTGTTCAGCAAAAGATTTTATTGAAAGAAAAAAGTCTCTGGGAAATAACTGTTGACGCTCTTGAA CACCTGACAGAGAAAGGACTTCTACAGAAAGACAGCTGTGGCGACAATGAGGGGTTAGAGTGTCACTTCC GGATTACGAAACTGGGCCAAGCTTCTTTTAAGGGAGCCATTGATTTGGCCTATTGTGACACTCTGTACAG AGACTTGAAGAAAGGTCTGGAAGGACTTGTACTGGAGAGCCTTCTTCATCTGATCTACCTAACCACGCCC TATGACCTGGCTGCTCAGTCTGAGCCTGACTGGATGGTGTACTTCAAGCAGTTTGGTCAACTCAGCCCAA CAGAGCAGAATGTAGCCGCTCTTCTCGGAGTCTCCGAAAGCTTTATCGGGAAGAAAGCAGCAGGACAAGC CGTCAGAAAGAAGGTGGACAAGAACGTTGTCAACAGACTCTACCTGTCGTTTGTGCTTTATTCTTTGCTG AAAGAGACCAATGTTTGGAGTGTGTCTGAGAAGTTTAACCTGCCCCGAGGATACATACAGAACCTGCTTA TGGGAGCTGCCTCCTTCTCTTCCTGCGTGTTACATTTCTGTGAGGAGCTGGAGGAATTTTGGGTTTACAA AGCCCTTTTGGTGGAACTCACCAAGAAGCTGACGTACTGCGTGAAGGCGGAGCTAATCCCTCTCATGGAA GTGACCGGGGTCTTAGAGGGTCGAGCAAAACAGCTATATAACGCAGGTTACAGGAGCATCATGCACTTAG CCAATGCCAACCCAGAAGTGTTGGTGAAGACAATTGATCATCTGTCCAGACGGCAAGCCAGGCAGATTGT TTCCTCAGCCAAGATGCTGTTGCATGAGAAGGCGGAGGCACTGCAAGGAGAAGCAGAAGAATTACTGAGA TTGCCTGCAGACCTCCCTGGGCTTGGGGGTCCCAGCTCTGAAAGGGCAGGAAGCCATGCTGGGGACGTAA CTCTGAGCTAGGGCATTTGCCTGAGATGCCTCCGGCCCTGGAATAAATCCTCAGTACCTCCAAGACAGAA GAAAAGGCCGGGGGGTTGTAAACTGCCCAGTGGTGAGCACCTGTAACGCAGCACTCGGGAGACAGGACGG ACAATGAGTGTCAGAAATCAGCTTCAGCTACACTGAGAGTTGGAGGCTAGCCTGGGCTGCATGAGTCCCT GTCTCCAAAAGAAAAAACCCCATGAAGCTGTCTGATGTATGTTTTTTAATTTGACTTATTTATTATATAT ATATTTTATTATTGAAAATCCCCCGTGTAAATGAAATGTGTATTTTAATTATACATTAAAAGCTGTGGAT GAGCTTTTAATAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_001081107
• Insert Size: 3210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082601, AAH82601
• RefSeq Size: 3672 bp
• RefSeq ORF: 3210 bp
• Locus ID: 191578
• UniProt ID: Q2VPA6
• Cytogenetics: 5 E4
• Gene Summary: Single-stranded DNA-dependent ATPase and 5' to 3' DNA helicase. Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with POLN polymerase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links.[UniProtKB/Swiss-Prot Function]