Fgf1 (NM_010197) Mouse Untagged Clone

CAT#: MC203263

Fgf1 (untagged) - Mouse fibroblast growth factor 1 (Fgf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgf1
• Synonyms: Dffrx; Fam; Fgf-1; Fgfa
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037601
  CCACGCGTCCGCCCACGCGTCCGGGCGTGGGAGAAGCAAGGCCTGGGCTTCCAGCGCGCTGTAGCAAGGT GTTGGTGCTTCCCACAGCAGTAGGAATGCATTGGAGGGGTGTGACAATGGAACTCAGGTGACACTGCTGA TGAAGGAGCCCAAAGAACCAATATCTGACCTGTGCTGAGCCTACCACCGCTGCTTGCTGCCGAGCCATGG CTGAAGGGGAGATCACAACCTTCGCAGCCCTGACCGAGAGGTTCAACCTGCCTCTAGGAAACTACAAAAA GCCCAAACTGCTCTACTGCAGCAACGGGGGCCACTTCTTGAGGATCCTTCCTGATGGCACCGTGGATGGG ACAAGGGACAGGAGCGACCAGCACATTCAGCTGCAGCTCAGTGCGGAAAGTGCGGGCGAAGTGTATATAA AGGGTACGGAGACCGGCCAGTACTTGGCCATGGACACCGAAGGGCTTTTATACGGCTCGCAGACACCAAA TGAGGAATGTCTGTTCCTGGAAAGGCTGGAAGAAAACCATTATAACACTTACACCTCCAAGAAGCATGCG GAGAAGAACTGGTTTGTGGGCCTCAAGAAGAACGGGAGCTGTAAGCGCGGTCCTCGGACTCACTATGGCC AGAAAGCCATCTTGTTTCTGCCCCTCCCGGTGTCTTCTGACTAGAGGAGTCTGTTCTGAGTGTTCTCTAT TTTGGTTGACCCTACCATGTTCCCTTGACCATTGGCTGCGCTAACCCTCAGGCCACAGAGCCTGAATTTG TAAGCAGTGCTTCTAAAATAGCCAGTTCACTTTTCTGCTGAGCCCTTCCCCACCACCCCAGCACAATTTG GAACACAGGGACCAAATTGCTTCAAGAGGACAACTGGCTGGCCAGTCTAGGTCTGGGTTTGGATATCCGG ATGCCTCAGGGCACCTGCTACAAGCTGGACCTGGTGATGCAAAGTTGGGGGCCATACGAAGCATTCAAAG GGGTCTAAGAAGTGGGTTCTTAGAAAGATACCCACTGCATGCTCTTTCCCCTTCTGAGCAATCCCTGGTT TTAAGCACCCCAGGCAATTAGCATGACTACCCTTCCCCCTACCAGAACCGCACAAATATCAGTAAAGCAA CCGGTAGTGAGGCTGAAGGAATTTCATGGTAGAGAGAAACCCCCCCCCCCCAGCCCCCACATATGGACAA GACTTAAAACCAAAAGGGAGTGTGGCGTTTCCGGGACAGCCTAGAAGCATGGGATCTCGGAACAGAGTAA GAAGGCAAGAGTCAGCCACAGGGAGTCAGGATGAGCCCACAGCCCAGCAGTTATCACCAAGTTACCAAGG AAACGTCCACAGTCAGGGAAAGAGCCCCGGAAATGGTTTCTTAGTAATGGCAAGCACAGAGATCTGGGGT CTGAAGAGTGGGCGTAGGAGCAAGGAGACAGGATGTCTTCAGCCGTGGATGTTAGCCTCTAAAAAGGAAG ATGTTAGACGGGAGGAGGAGGAGAGAAAAAGTAATATCACGTCACTGTGTGCTTGGCCACCACGAAACTG GGGTTGTGGGGGTCCCAGTACGATGGCCCCAAATAGCCCATACATTCAGATCATCTGTATTCAGATTCAT TTGTATGACTGTGAAACCTGATTTGGTGGGGCTGGTGTGGGGGAGGTCTGTGTCTTGAAAGGCCCGCAAG TCCCATAGATGCTGCTGGCTTCCCCACCATGCTTTCAAGAGCAGCCCCAGAGAATGCTGTTCAAATGAAG GTTGCTCAGACTTCACCCTCTTTTACCTCCAGTGCCCGGGTTTTCCTGAGTATCCATCCAGGCAGTGTCC ATAGTTAGTGAAGCATGGTTTCAAGGCACCTTGGCCCAGTGCCTATAAAATGCCCACTCAGAAATCACTG AAACCGTACTTCAAAAAAAAATGTTGAACCTCACCAAAAGCTTTCTCCCAAGAGACCATTTCTGTTACCA TTAATTCTTCAAACTGGCTTCCTCCTTAAAGTAAACCTGGCCCAAGTGCCCAGCAAATTAGAAACACTAT TCATCTCTGACATAGGACACAAACGCCATGAGAAGGCCTTTCATACGCCAATTTAATATACTGTGAGACT TATCTTCTCGTTGCTTCTTATATAGCTTGAAGGCATTAAATAGATTCACAATCAGCCACAGAGGATAATA ACAGCCTAGAATGTAAAAGCTACTTAGTTATCTAACATGCTACATTCATTCATGAGGCCTTTCCTCTTTG TCTTCCACTGTGCTTTAAGAATGGGTATCTAGGGGCTGGTGAGATGGGACAGGAGTTCAGTTCCTAGCAT TCACAGTGTGATGCTCAGACCCAATTCTAACTCCAACTCCAGGGGTACTGCATACACATGTACAAACCCA CACTTTTTCAGAAATATAAACCAATAATATGGTTGTGGATTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010197
• Insert Size: 468 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037601, AAH37601
• RefSeq Size: 2440 bp
• RefSeq ORF: 468 bp
• Locus ID: 14164
• UniProt ID: P61148
• Cytogenetics: 18 20.74 cM
• Gene Summary: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.[UniProtKB/Swiss-Prot Function]