Spata7 (NM_178914) Mouse Untagged Clone

CAT#: MC203259

Spata7 (untagged) - Mouse spermatogenesis associated 7 (Spata7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spata7
• Synonyms: AI661438; B230306G18Rik; HSD3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046960
  CGACTGTCGCTGTGACCCTTTGCGCCCCCGCGGTCCTGGTCTTTTCCTTTTCACCTCCGCTGCGGGGGAG CTCGGCTTCATCCCAGTAAGAACCAAGGGGATACAACCGCGGAGGCCGCAGGAGGACTAAGCATGGATGG CAGCCGGAGAGTCAGAGCAACCTCTGTCCTCCCCAGATACAGCCCTCCATGCTTGTTTACAGGACACCTG AGCACCAAGAGCAACGCTTTTTGCACTGATTCCTCTTCTCTCAGACTAAGCACCCTGCAGCTGGTCAAGA ACCACATGGCTATTCACTATAATAAAATCCTTTCAGCCAAAGCCGCAGTGGACTGCTCAATTCCAGTAAG TGTGAATACTAGCATCAAATATGCTGACCAACAACGAAGAGAAAAACTGAGGAAGGAGTTGGCACGGTGT GAAAAGGAGTTTAAATTAAGTAAATCTGCTATGCAGACCAATTCTAAGATGAATTCCAAGTTCTTTGTAA ATTCTCTACAGAAGCCCTCAGGGGAACCACAAGACCAGGATGTGTTCATAGAAGAAATGACAAGATACCC ATCATTTTCAAAGTCACTCATCCCTTCTTCCGAGGGACTGCATCTAAGTCTGCCTGAATCAAGTAAGATG CTCATGAGTGGCACCCAGAAGCATGCCAGCACCTCTCCATCCAGACACTCAGGCTGTGGGCATGGCTGCG ACAGGCGGCCTCGGAGTGCCCACCAGTTCCAGGTAGCCCTCGCCAAGACCCCAAGTGGAGATCTGTTGGA AAAGCACTCTGACCTCTTCTCTAACAAGCAGTCGCCATTCACTCCACGAACTTTAAAAACAGAAGCAAAG TCCTTCCTGTCACAGTACCGCTACTACACACCTGCCAAAAGAAGAAAGGATTTTTCAGATCAGCGGATGG AAGCTGAAACCCAAACTGAATTAAGCAGCTTTAACTCTGAGCTTGGGACAGCTGAGAAAACGAGCTCAAA GGACTCAGAAGTGAACATAAACCAGGTACCTAATTACACGAGAAATGGTGCTGAAGACAAAATAGCTCCT TTACCCTCACAAGGACAAAACTTAGCATGGGACAGTATTCAAGATGGGATTCTGCAGCAGTCCTCAGAAA GGGCATCCTGTAAGCTCTCCACAGAGTTTTCTCCAGACAGTAAAATCTACTCTGATGAAGAAGAGCTGCT GTACCTGAGTTTCATGGAAAATGTGACGGATGAAATCTTGAAACTTGGTTTATTTTCTAACAGGTTTCTA GAGCGACTGTTTGAGCGACATATAAAGAAAAATAAACATCATTTGGAGGAGGGAAAGATGCGCTACCTGC TGCATGGGCTGAAGGTTGACTTAGGCTGCATATCTGAGGAAGACCCAGCAAAGCAAAAACATTTCAGAAT GTTGAATCAACTTCATTTTCAAAAGGCTCTGATTTCAAGAGAAAATGAGTTTGTAAGTGATGAAGAAACA GTGAGTCACCACGAGCGCCAGCAGTACCAGGAGGCCCTGGACATGCTGTCGGCTGTGCCGAAGGACGAAA ACAAGATGTTCTCTTTACCGGGCGAATTTTTAATACCCGCCCATAAAGTCAAGCATTCAGAGGGTGTTAT AATTCAACAGGTCAATGACGAAACAGATAATGAAGCCTCACCCTGGAATGAAAACAATCCAAGTGTTTCT GATAGTGTAATAGACCAGGAAACCTCTGTGGATGTCATTGAAGGTGACAGTGACTTTGAAAGGGCTGAGA CTTCCAGGGAACTCTGTTGTCTGAGCACATCACTGTCCCCATCTGGTCCATTCCCCAGCATCAATGGTGG CAGTAATCATGGTAAGGAATTATCGACTCTACGAATCATGGGAATGAGCATTGAAGACTGACCTCTGGAT CTACATTAATAAACACTCCATGTGGCCAGTAACTCAATATTACTTTTCTTCTTTTTTTTTGTCCCCTTTT ACATATTAAGATTTCTGTAATTTCAATGCTATGTAATAAATTACCACAATTTTAGTGTCTTGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178914
• Insert Size: 1749 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046960, AAH46960
• RefSeq Size: 2076 bp
• RefSeq ORF: 1749 bp
• Locus ID: 104871
• UniProt ID: Q80VP2
• Cytogenetics: 12 E
• Gene Summary: Involved in the maintenance of both rod and cone photoreceptor cells (PubMed:25398945, PubMed:29100828, PubMed:29899041). Required for photoreceptor-specific localization of proximal connecting cilium (CC) proteins RPGR, AHI1, NPHP1, NPHP4, and RPGRIP1 at the distal CC, a photoreceptor-specific extension of the primary cilium transition zone (PubMed:25398945, PubMed:29100828, PubMed:29899041). Maintenance of protein localization at the photoreceptor-specific distal CC is essential for normal microtubule stability and to prevent photoreceptor degeneration (PubMed:25398945, PubMed:29899041).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1).