Sptlc1 (NM_009269) Mouse Untagged Clone

CAT#: MC203256

Sptlc1 (untagged) - Mouse serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sptlc1
• Synonyms: AW552086; C77762; E030036H05; Lcb1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046323
  GGCTGCCTGACCATGGCGACAGTGGCGGAGCAGTGGGTGCTGGTGGAGATGGTGCAGGCGCTGTACGAGG CTCCAGCATACCATCTTATTTTGGAAGGAATCCTAATACTTTGGATAATCAGACTCGTTTTCTCTAAAAC TTACAAGTTGCAGGAGCGTTCTGATCTTACAGCCAAGGAAAAGGAAGAACTGATTGAAGAGTGGCAGCCA GAGCCCCTCGTCCCTCCAGTCTCCAAGAACCACCCTGCTCTCAACTACAACATCGTGTCCGGCCCTCCAA CCCACAACATCGTGGTGAATGGAAAAGAGTGTGTCAACTTTGCCTCCTTTAACTTCCTTGGGCTGCTGGC CAACCCTCGAGTTAAGGCCACAGCTTTTTCATCTTTAAAGAAGTACGGAGTGGGTACCTGTGGTCCTCGA GGGTTCTATGGCACATTTGATGTCCATCTGGATTTAGAAGAGCGCCTGGCAAAGTTTATGAAGACCGAAG AAGCCATCATTTACTCGTATGGCTTCTCCACCATAGCCAGTGCCATTCCTGCGTACTCTAAGAGAGGGGA CATCATCTTTGTGGACAGTGCGGCCTGCTTTGCTATCCAGAAAGGATTACAGGCATCAAGAAGTGACATT AAGTTATTTAAGCATAATGATGTGGCTGACCTGGAGCGACTGCTAAAAGAACAAGAGATTGAAGATCAAA AGAATCCTCGCAAGGCCCGTGTGACTCGGCGGTTCATCGTGGTGGAGGGCTTGTACATGAACACTGGAAC CATCTGTCCCCTTCCAGAACTGGTTAAATTAAAATATAAATATAAAGCAAGAATATTCCTGGAGGAGAGC CTGTCCTTTGGAGTCCTTGGGGAGCACGGGCGAGGAGTCACCGAGCACTATGGGATCAGTATTGATGATA TTGACCTTATCAGTGCTAACATGGAGAATGCACTCGCTTCTGTCGGGGGCTTCTGCTGTGGCCGCTCCTT CGTGGTTGACCATCAGCGGCTCTCCGGTCAAGGATACTGCTTTTCTGCTTCGTTACCTCCCCTGCTAGCT GCTGCTGCCATTGAGGCCCTCAACATCATGGAAGAGAATCCAGATATTTTTGCAGTTTTAAAGAAAAAAT GCCAGAACATCCATAAGTCTCTACAAGGTGTTTCGGGTTTAAAAGTGGTGGGAGAGTCCCTTTCTCCGGC GCTTCATCTCCAGCTGGAAGAGAGCACGGGCTCTCGGGAGAAAGATGTGAAGCTGCTTCAAGCAATCGTA GATCAGTGCATGGATAAGGGCATCGCATTGACTCAGGCACGGTACTTGGACAAGGAAGAGAAGTGCCTTC CTCCTCCAAGCATCAGGGTTGTGGTCACTGTGGAGCAGACGGAGGAAGAGCTACAGAGGGCTGCATCCAC CATCAGGGAGGCGGCCCAGGCTGTGCTGTTGTAGGCTCCTGCCCAGTGCTTCAGCCATGTCACCTGCGGA CGGAACTACTCAGAGACCTCCAGCTGCCCAGGGTGTGGCTGTGCTTGAATACACAGCCAGAGTGGATGGT TGTATCCAGCAGGAGCTGACTTGTAGAGGATGACAGATGACAGCATATCCCTGGTGTTTCTGCAGTGCCG TGTGCTCTGTCAGGATGCTTCACTGTCATTTGTATTCGCTTTTGTTTAAAACTGGAGCTTCCATTTTCCC TCTGTCGTCTGTGGGAATGATTCAATAAGCACTTCTCCGTATCCATAGAAAAGGACTTTGCAGCTAAGAG GGACTCTCCATGTATCCAGTTACTGTGGGAGACTGAGAGACTGTGTCCTCCCCTGTCCTCAGGCAGAGCT CAGTGGTGGCTGCTATGCAGATACTCTGTGTGTGTTATAAACGTCATACAACATGGTGATCCTAGGACTC TATGTGCTCCAGTTACTGATACTGTCCTGTCTAATCTGAGCTTCACTAACAGATCTTCATACCGCTTCCT AAGCAATAATCTGAATCAAACGTTATTTTATTTTTGTGTAATTGACTTTATATTTTTTATATATTCTCTA GTCAGTTTTGGGAAGAGGGTTATTATTCTGATCCCCAAATACAAATCATGCCCTTGGTATTTTCTAATAT GTCCAATGTCCTTTTTCATGGTGGTTTTGTCCAGGAGCATAGACCTGTGGGCTTTTTCTAAGCTGAGAAA AATGAGACCTTTCCTCTTTCACTCTATAAATTCCAGATGCCTCCGAAAAATAGGGATGCTCTAAACGTGA TTTCCGAGCTCTACACTGGCTGCCGTTGTCCTTAGCGAGCGTGTTGAGTGATATAAAGGCTTGTGACATG TGCCACGGATCGTGTGCATGGAAGGCCTTCTCTTGTTGCACGGGCAGAGGAGTCAGGTCATTTAAATGTC ATTTTCTGATGTGCGTTCATGTGAACCATGGGTCGGCATCTTCTTAAGTGATCTGTGACATTTTTAATGT TTCTATTTCATACGTGCCTTGTGATTGCTGCTGCTGTGAGGGTACACTCCTGTCGGGGAAAAGTCTGTTT TGGTTTGGTTTTTAATGAAATAAACCTCCAAGAGCTTACATTCCTGAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009269
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046323, AAH46323
• RefSeq Size: 2601 bp
• RefSeq ORF: 1422 bp
• Locus ID: 268656
• UniProt ID: O35704
• Cytogenetics: 13 B1
• Gene Summary: Serine palmitoyltransferase (SPT) (PubMed:28100772). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity). Required for adipocyte cell viability and metabolic homeostasis (PubMed:28100772).[UniProtKB/Swiss-Prot Function]