Tlr4 (NM_021297) Mouse Untagged Clone

CAT#: MC203163

Tlr4 (untagged) - Mouse toll-like receptor 4 (Tlr4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlr4
• Synonyms: Lps; Ly87; Ran/M1; Ras; Rasl2-8
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC029856
  GTTCTACTCTTCAGTCTTCTAACTTCCCTCCTGCGACGGGGCAGATCGATTCTAGAACAAAACCAAAAGT GCGAATGCTAAGGTTGGCACTCTCACTTCCTCTTTGAATATAGTACTTGCAGAGGGGCACCCACTGGGAG GGAAGAGGCAGGTGTCCCAGGGACTCTGCGCTGCCACCAGTTACAGATCGTCATGTTCTCTCATGGCCTC CACTGGTTGCAGAAAATGCCAGGATGATGCCTCCCTGGCTCCTGGCTAGGACTCTGATCATGGCACTGTT CTTCTCCTGCCTGACACCGGGAAGCTTGAATCCCTGCATAGAGGTAGTTCCTAATATTACCTACCAATGC ATGGATCAGAAACTCAGCAAAGTCCCTGATGACATTCCTTCTTCAACCAAGAACATAGATCTGAGCTTCA ACCCCTTGAAGATCTTAAAAAGCTATAGCTTCTCCAATTTTTCAGAACTTCAGTGGCTGGATTTATCCAG GTGTGAAATTGAAACAATTGAAGACAAGGCATGGCATGGCTTACACCACCTCTCAAACTTGATACTGACA GGAAACCCTATCCAGAGTTTTTCCCCAGGAAGTTTCTCTGGACTAACAAGTTTAGAGAATCTGGTGGCTG TGGAGACAAAATTGGCCTCTCTAGAAAGCTTCCCTATTGGACAGCTTATAACCTTAAAGAAACTCAATGT GGCTCACAATTTTATACATTCCTGTAAGTTACCTGCATATTTTTCCAATCTGACGAACCTAGTACATGTG GATCTTTCTTATAACTATATTCAAACTATTACTGTCAATGACTTACAGTTTCTACGTGAAAATCCACAAG TCAATCTCTCTTTAGACATATCTTTGAACCCAATTGACTTCATTCAAGACCAAGCCTTTCAGGGAATTAA GCTCCATGAACTGACTCTAAGAGGTAATTTTAATAGCTCAAATATAATGAAAACTTGCCTTCAAAACCTG GCTGGTTTACACGTCCATCGGTTGATCTTGGGAGAATTTAAAGATGAAAGGAATCTGGAAATTTTTGAAC CCTCTATCATGGAAGGACTATGTGATGTGACCATTGATGAGTTCAGGTTAACACATACAAATGATTTTTC AGATGATATTGTTAAGTTCCATTGCTTGGCGAATGTTTCTGCAATGTCTCTGGCAGGTGTATCTATAAAA TATCTAGAAGATGTTCCTAAACATTTCAAATGGCAATCCTTATCAATCATTAGATGTCAACTTAAGCAGT TTCCAACTCTGGATCTACCCTTTCTTAAAAGTTTGACTTTAACTATGAACAAAGGGTCTATCAGTTTTAA AAAAGTGGCCCTGCCAAGTCTCAGCTATCTAGATCTTAGTAGAAATGCACTGAGCTTTAGTGGTTGCTGT TCTTATTCTGATTTGGGAACAAACAGCCTGAGACACTTAGACCTCAGCTTCAATGGTGCCATCATTATGA GTGCCAATTTCATGGGTCTAGAAGAGCTGCAGCACCTGGATTTTCAGCACTCTACTTTAAAAAGGGTCAC AGAATTCTCAGCGTTCTTATCCCTTGAAAAGCTACTTTACCTTGACATCTCTTATACTAACACCAAAATT GACTTCGATGGTATATTTCTTGGCTTGACCAGTCTCAACACATTAAAAATGGCTGGCAATTCTTTCAAAG ACAACACCCTTTCAAATGTCTTTGCAAACACAACAAACTTGACATTCCTGGATCTTTCTAAATGTCAATT GGAACAAATATCTTGGGGGGTATTTGACACCCTCCATAGACTTCAATTATTAAATATGAGTCACAACAAT CTATTGTTTTTGGATTCATCCCATTATAACCAGCTGTATTCCCTCAGCACTCTTGATTGCAGTTTCAATC GCATAGAGACATCTAAAGGAATACTGCAACATTTTCCAAAGAGTCTAGCCTTCTTCAATCTTACTAATAA TTCTGTTGCTTGTATATGTGAACATCAGAAATTCCTGCAGTGGGTCAAGGACCAGAAGCAGTTCTTGGTG AATGTTGAACAAATGACATGTGCAACACCTGTAGAGATGAATACCTCCTTAGTGTTGGATTTTAATAATT CTACCTGTTATATGTACAAGACAATCATCAGTGTGTCAGTGATCAGTGTGATTGTGGTATCCACTGTAGC ATTTCTGATATACCACTTCTATTTTCACCTGATACTTATTGCTGGCTGTAAAAAGTACAGCAGAGGAGAA AGCATCTATGATGCATTTGTGATCTACTCGAGTCAGAATGAGGACTGGGTGAGAAATGAGCTAGTAAAGA ATTTAGAAGAAGGAGTGCCCCGCTTTCACCTCTGCCTTCACTACAGAGACTTTATTCCTGGTGTAGCCAT TGCTGCCAACATCATCCAGGAAGGCTTCCACAAGAGCCGGAAGGTTATTGTGGTAGTGTCTAGACACTTT ATTCAGAGCCGTTGGTGTATCTTTGAATATGAGATTGCTCAAACATGGCAGTTTCTGAGCAGCCGCTCTG GCATCATCTTCATTGTCCTTGAGAAGGTTGAGAAGTCCCTGCTGAGGCAGCAGGTGGAATTGTATCGCCT TCTTAGCAGAAACACCTACCTGGAATGGGAGGACAATCCTCTGGGGAGGCACATCTTCTGGAGAAGACTT AAAAAGGCCCTATTGGATGGAAAAGCCTCGAATCCTGAGCAAACAGCAGAGGAAGAACAAGAAACGGCAA CTTGGACCTGAGGAGAACAAAACTCTGGGGCCTAAACCCAGTCTGTTTGCAATTAATAAATGCTACAGCT CACCTGGGGCTCTGCTATGGACCGAGAGCCCATGGAACACATGGCTGCTAAGCTATAGCATGGACCTTAC CGGGCAGAAGGAAGTAGCACTGATGCCTTCCTTTCCAGGGATATGAATTACCTAACTCAGGAAAAGAAAC ATAATCAAGAATCTTTACCTTTAAACTGAAGGAGAAGAGGCTAAGGCCTAGTGAGAACAGAAAGGAGAAC CAGTCTTCACTGGGCCTTTTGAATACAAGCCATGTCATGTTCTGTGTTTCAGTTGCTTTAGAAGAGTATT GATAGTTTCAACTGAACTGAAAGGTTTCTTACTTTCCCTTTTTTCTACTGAATGCAATATTAAATAGCTC TTTTTGAGAGGTCCTCATTCCAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021297
• Insert Size: 2508 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029856, AAH29856
• RefSeq Size: 3187 bp
• RefSeq ORF: 2508 bp
• Locus ID: 21898
• UniProt ID: Q9QUK6
• Cytogenetics: 4 34.66 cM
• Gene Summary: This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]