Pno1 (NM_025443) Mouse Untagged Clone

CAT#: MC203134

Pno1 (untagged) - Mouse partner of NOB1 homolog (S. cerevisiae) (Pno1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pno1
• Synonyms: 1810003N24Rik; AA407421; AU015468; AU019495; AU042323; Emi3; Imi3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020037
  CGGACGCGTGGGCAACCACGCACGTGTTCCTCTAGTCGTACTTGCCGGGATTGATCTTGGCCGAGATGGA GACGCAAAGCACCGGGACGGAGGACGGCTTTACTCCCGTCACGCACAGAGGGGGCCGCCGGGCGAAGAAA CGACAGGCTGAGCAGTCGTCAGCGGCGGGACAGGATGGAGAAGCCGGACGCATGGACACGGAGGAGGCGC GGCCGGCGAAGAGGCCGGTCTTCCCGCCGCTCTCCGGGGATCAGCTCCTGACTGGGAAAGAAGAAACGAG GAAAATTCCCGTCCCAGGTAACAGATACACACCGTTGAAAGAAAACTGGATGAAGATATTTACTCCGATT GTAGAACATTTGGGACTTCAGATACGCTTTAACCTGAAATCAAGGAATGTAGAAATCAGGACTTGTAAAG ACACCAAGGATGTCAGTGCCCTGACAAAAGCGGCCGACTTTGTGAAAGCCTTTGTTCTTGGCTTTCAGGT GGAGGATGCACTTGCCCTTATCCGACTAGATGACCTCTTCCTAGAGTCTTTTGAAATAACTGATGTTAAG CCCCTAAAAGGAGACCACCTGTCAAGGGCAATAGGAAGAATTGCTGGCAAAGGAGGAAAAACCAAATTTA CCATAGAGAACGTAACACGGACACGGATTGTCCTGGCAGATGTGCACGTTCACATTCTTGGCTCTTTCCA AAATATCAAGATGGCAAGAACAGCTATTTGCAACCTCATCCTAGGAAATCCTCCATCAAAGGTGTATGGC AATATCCGAGCTGTGGCCAGCAGATCAGCAGATCGGTTCTGATTTTCCATGAACCCTTGAGATGTTCACA TAAAACCATGGGAAGAATTTCCCAGCCACTTAGAGCTCAGGTTTCTTCTCAGCTCTCAGCCAGAGCAGAA GCATAAAAGGAAGGTTTAACAGGACTTGTACTCAGCTGCTTCTAGGATAATTTAAATATGATATAATACT GAAAAGTACAGCAGTTTATACTTAAAGTGTAAGTAAGCCACACGGGCAGATGCACAGGTTACAACCCTAT CAATTAGAGGTAACTGCTTGCTTACAACTCTTTGGTTACAGATTTACAGCTAAACTTATAAACTTTATAT AAACTCTGAAATTTTTAATTAAAAATGAATCACAATTCTTGGTGCCAGGCACTGTGACTATTTCAGGGTT GAGTCATGGCAGAAACTTGCACTATTGAACTATATGTGCTCATCTTTGTACTTCCTGTACAATTTATAAA ATGAAAGTCCTGCATCACGTGGCTAATAAATGTTATTTTTCAAATAATTAAAGATGTTAAAATATTTCTC ATGTGCAGTAAAAGTAAAAATGTAAAAGAATTCTCCCAAGAAAACTGTTCTCCAAACAGCATATTCTACT AGTGTCTGTGTGTATGAATTTGATTATTTCTTAAATTGTTTTAGAACACATTTCATTAAACATGTAACTT AGATACTACATTCTCATGTTTCTCAAAAGCCTTAACCAAAATATTGTAATGATTGGAGATCAGTAAATTC AAGCAATAACTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025443
• Insert Size: 747 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020037, AAH20037
• RefSeq Size: 1567 bp
• RefSeq ORF: 747 bp
• Locus ID: 66249
• UniProt ID: Q9CPS7
• Cytogenetics: 11 A2
• Gene Summary: Positively regulates dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA.[UniProtKB/Swiss-Prot Function]