Pofut1 (NM_080463) Mouse Untagged Clone

CAT#: MC203080

Pofut1 (untagged) - Mouse protein O-fucosyltransferase 1 (Pofut1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pofut1
• Synonyms: mKIAA0180; O-FucT-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046295
  GCTCCCACCCCCTCCCCGCCTCCGGGCCGTGGCACTCTGGGGCTCTGCCGTCGACATGGGCGCCGCCGCG TGGGCACCGCCACACCTGCTGCTGCGGGCGTCTTTCCTGCTTCTGCTGCTGTTGCTGCCGCTCCGCGGGC GGTCAGCGGGCTCCTGGGACCTGGCCGGTTACCTGCTCTACTGTCCCTGCATGGGGCGCTTTGGGAACCA GGCTGATCACTTCTTGGGCTCCCTGGCATTTGCGAAGCTGCTGAACCGCACCTTGGCTGTACCTCCATGG ATTGAATACCAACATCACAAGCCTCCTTTCACCAACCTCCATGTGTCCTACCAAAAGTACTTCAAACTGG AGCCTCTCCAAGCCTACCATCGGGTTGTCAGCCTGGAGGACTTCATGGAAAATCTGGCACCCTCCCACTG GCCCCCTGAGAAGCGAGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGTCCTGATAAGAAGACATGT CCCATGAAGGAAGGAAATCCTTTTGGGCCATTCTGGGACCAGTTTCATGTGAGTTTCAATAAGTCAGAAC TGTTCACAGGCATTTCCTTCAGCGCCTCCTACAAAGAACAATGGACCCAGAGATTTCCTGCAAAAGAGCA TCCTGTGCTCGCACTGCCTGGGGCCCCAGCACAGTTCCCTGTCCTGGAGGAACACAGGGAGCTCCAGAAG TACATGGTGTGGTCAGATGAGATGGTGAGGACGGGAGAGGCCCTGATCAGTGCCCACCTCGTCCGGCCCT ATGTGGGCATTCATCTGCGCATTGGCTCCGACTGGAAGAATGCCTGTGCCATGCTGAAGGATGGAACTGC AGGGTCACACTTCATGGCTTCCCCTCAGTGTGTGGGCTATAGCCGCAGCACAGCGACCCCTCTCACCATG ACCATGTGCCTCCCTGACCTGAAGGAAATCCAGCGGGCTGTGACGCTTTGGGTGAGAGCACTGAATGCCA GATCGGTCTACATCGCCACAGACTCTGAGAGCTACGTGTCAGAGATCCAGCAGCTCTTCAAAGACAAGGT GAGGGTGGTGAGCCTGAAACCCGAGGTGGCCCAGATCGACCTGTACATCCTCGGCCAGGCTGACCACTTC ATTGGAAACTGTGTCTCCTCGTTCACTGCCTTCGTGAAGCGGGAGCGGGACCTCCATGGGAGGCAGTCGT CCTTCTTTGGCATGGACAGACCCTCCCAGCTTCGGGATGAATTTTGATCCCATCTGAAGCCCCCACCTCC CACCCTCAACCAGGCTTGGCTGCTAAGGATGCTCCTGGGATTACACATCCCTTTTCTTTTCTGCCAGAGG TGGAGAACAGTACCAAGGACGTCCTGGAAGAGAAGGCATTCCATCCCAGGCATTGGCTCCCATGGTCCCC AGCCAGGGCTGCTCTGGGCTGCATGATCATGCACATACAAAGCAGCCCACCTCCAGCCTGGGCACACCCT GATATATACACATACATACACATAATATATATCATATATATATAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_080463
• Insert Size: 1182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046295, AAH46295
• RefSeq Size: 1529 bp
• RefSeq ORF: 1182 bp
• Locus ID: 140484
• UniProt ID: Q91ZW2
• Cytogenetics: 2 H1
• Gene Summary: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).[UniProtKB/Swiss-Prot Function]