Nek7 (NM_021605) Mouse Untagged Clone

CAT#: MC203059

Nek7 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nek7
• Synonyms: 2810460C19Rik; AU020186
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037697
  CCACGCGTCCGCGCGGGCCGAGCAGGGTGGGAGCCGGCGCCTGCAGTGGCGCGGGGAGAGGCGATCGGCC CCGGATCCCGCAGCCCTGCAGCCCGCGTAGACCGAGCTGCCCCGGCGCCCCGAATCCTGAAGTCCCAGTT GAGTCAGAATGGATGAACAATCACAAGGAATGCAAGGGCCGCCCGTTACTCAGTTCCAGCCACAGAAGGC ATTACGGCCAGATATGGGCTATAATACTTTAGCCAACTTCCGAATAGAAAAGAAAATTGGTCGTGGACAA TTTAGTGAAGTTTATAGAGCATCCTGTCTCTTGGATGGAGTGCCGGTAGCGTTAAAAAAAGTACAGATAT TTGATTTAATGGATGCCAAAGCACGTGCTGATTGTATCAAAGAAATAGACCTCCTTAAGCAACTCAACCA TCCAAATGTAATTAAATACTATGCATCATTCATTGAGGATAATGAGCTGAACATAGTTTTGGAGTTAGCA GATGCTGGTGACCTCTCCAGAATGATAAAGCACTTTAAGAAACAAAAGAGGCTAATCCCTGAGAGAACCG TTTGGAAATACTTCGTTCAGCTCTGCAGTGCACTGGACCACATGCATTCTCGAAGAGTCATGCACAGAGA TATAAAACCAGCTAATGTGTTCATTACAGCCACTGGGGTAGTAAAACTCGGAGACCTTGGGCTTGGTCGG TTTTTCAGCTCCAAAACCACAGCTGCACATTCTTTAGTGGGTACACCTTACTACATGTCTCCAGAGAGAA TACATGAAAATGGATACAACTTCAAGTCTGACATCTGGTCTCTTGGCTGTCTGCTATATGAGATGGCTGC ACTGCAGAGTCCTTTCTACGGCGACAAGATGAACTTGTATTCTCTGTGTAAGAAGATAGAGCAGTGTGAC TACCCGCCTCTCCCGTCAGATCACTATTCGGAGGAGCTACGACAGCTAGTTAATATATGCATCAACCCAG ATCCAGAGAAGCGACCCGACATCGCCTATGTTTATGATGTGGCAAAGAGGATGCATGCATGTACCGCAAG CACCTAAACTGTACAAGATCCTGAAGACGGCAACCAAGATAACTTAAAAGTGTTTTTGTGCAGATCATAC CTCCCCGCTTATGTCTGGGTGTTAAGATTACTGTCTCAGAGCTAATGCGCTTTGAATCCTTAACCAGTTT TCATATGAGCTTCATTTTTCTACCAGGCTCAATCACCTTCCCAATCCACAACTTTGGGATGCTCAGATGG CACCAAGAATGCAAGCCCAACAAGAGTTTTTCGTTTGAGAATTGTTTCGAGTTTCTGCTGATAGACTGTG TTTATAGATAGTCAGTGCCCGATGGTGAAGCACACACACATAGGCACATGTCCAGAGCGATGCAGAACCT GAGGAAGGACCTGGGCATTTGACTTGTTTGCTTTTAAGTCACTTAATGGACGTTGTAGTGGACATGATTG TGAACTTCTGATTTTTTTCTTTTAAGTTTCAAGTACATGTTTTAGTTCTTAGCATTAGAGATCTCAAATA TCATTCTTATAAGACATGCAGACATAAACTTTTTGAGAAAGATTTAAAATTTTTAGTTTATACATTCAAA ATGCAACTATTAAATGTGAAAGCATAGAGGTCAAAATGTGAGTTGGACACTGAAGTCTATGTTTTAATGC CTTTGAAAGCCTTTTTTTTGTGTGTGTTTAAATGGTATAAATGAACCCATTTTAAAACGTGGTTAAGGAC TTGTTTGCCTGGCGTGATAGTCATGTTTAACATGCACAAGGCTTTGTGTTTTTATTGTACATTTGAAGAA TATTCTTGGAATAATCTTGCAGTAGTTATAGTTCAATTTCTTTACAAATCTAAATACACTTAACTCATAA CTATACACTGTAATGCAAGCATATATTGTTATTCATATATTGAAGTTTTGATCAGTTCCTCTTCAGAATC TTTTTTATCCAAGTTACTTTCTTATTTATATTGTGTGTGCATTTCATCCATTAAATGTTTCAGATTTTCT GAGAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021605
• Insert Size: 909 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037697, AAH37697
• RefSeq Size: 2059 bp
• RefSeq ORF: 909 bp
• Locus ID: 59125
• UniProt ID: Q9ES74
• Cytogenetics: 1 60.82 cM
• Gene Summary: Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).