Depdc1a (NM_029523) Mouse Untagged Clone

CAT#: MC203033

Depdc1a (untagged) - Mouse DEP domain containing 1a (Depdc1a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Depdc1a
• Synonyms: 5830484J08Rik; Depdc1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023934
  CCACGCGTCCGCAACTCCAGGCCACAGAGGTTCCTGCTACCGCGCACTTTCCGCAGCCTCTCAGCCCGGC AATGATAGCATGGAGCGTCAGGGTGCTCCTCTTGGACCTTACCGGGCCACCAAGCTGTGGAATGAAGTAA CCACATCTTTTCGAGTAGGAATGCCTTTAAGAAAACATAGACAACATTTAAAAAAATATAGCAATTGTTT CACTGCAGTTGAAGCTATAGATTGGCTTTATGATCTGTTAAGAAGTAATAGTAATTTTGGTCCTGAAGTT ACAAGACAACAGACTATTCAACTATTGAGAAAATTTCTTAAGAATCATGTGATTGAAGATATCAAAGGTA GATGGGGATCGGAAAGCCTTGATGACAACAATCAACTATTCAGATTTCCTGCAACTTCTCCACTTAAAAC TTTTCCACAAAGACATACAGAAATCAAGAAAACCAACACAGAGTACTTTTCCAAAGACAAAGATGGTATT TTTAAATTACGAAACTTATCTCAAAAAACTTCTAAGAAGCGTGGATTACATTTCTCTCAGGAAAATACAG AAAAGATAAATCATGAAAGAATAACCAATGAAGATCTAGAAATTGCACCTGACAACCAAGAAATAAGCCA GGAAGATGTTGAAGAAGTTTGGAGATACGTTATTATGATATACCTGCAAACCATTTTAAGTTTGCCATCC ATAGAAGAACTCTTAAACCCAAACCAAGTAATTCCTCAATATATAATGTACAACATGGCCAACACAAGTA AGCATGGAGTGGTCATACTACAAGACAAATCAGACGATCTTCCACATTGGGTTTTATCTGCAATGAAGTG CCTAGCAAATTGGCCAAGAAGTAATGACACCAATAATCTAACTTATGTTGGATTTGAACGTGATGTATTC AAAACAATTGCAGATTATTTTCTAAATCTCCCTGAACCTCTACTTACATTTGAATATTATGAATTATTTG TGAACATTTTGGTTGTTTGTGGCTACATCACAGTTTCAGATAGAACAAGTGGAATACATAAAATACAAGA TGATCCACGATCTTCAAAAATCCATGATTTAAGCAATTTGAATTCCTTCAAATCAACTGAGTGTCTTCTT CTCAGTCTGCTTTACAAAGACAAAAGCAATGAAGAGTTGGATTCTACTAAAAGACTACAAAGAAATGATC AAGGATTTCAAGAAAGATGTGCCAAGAAAATGCAGCTAGATAATTTAAGAAACAGAAGAGCTAGTGCTAA TGACATAATGGGAGGAAGCTGTCATAATTTAATAGGCTTAAGTAATACAAATGCCCTGTCCTCTAACATC AAACCAAGGTGCTCTTCTTTGGAAGGAATTGTAGATAGGCCAGTGAATTCAAGTGAAAAGAAGTCCAGTA TCTTCTACCAGTCTGTTCTGAACATAGAAGAACAAAATAGTAAGCAATCGTTAGTGTCTGCACCCAAACA GACACCCCTATTTAATCTTCACTCAGATGAAAATGCTCAGCAGCCACACTGTGTTGGTTTTAACAGAACC TCTGCTTTGACTGTTCAAGACCAAGAAGAGTTATGTAATGAAAAGTATAAGTCAAAACAGCTTTGTAGAT CTCAGAGTTTACTTTTAAGAAGTAGTACAAGACAGAATAGTTGTATCAATAAACCAGTGGCTGAAATTAT AATGAAACCAAATGTTGGACAAGGGAGTACAAGTGAGCTCGGAGAGTCTAGTACCACAATCAATAAAAGA CTGTGCAAAAGCACCATAGAACTTTCAGAGAAATCTTTACCTCCAGCTGCTTCTGTGTTGACTGGCACAC AAAGTTTGCTGCAACCTCACTTAGAGAGGGTTGCCATCAATGCTCTACAGTTATGTTGTTTGTTACTTCC CCCACCAAATCGTAGAAAGCTTCAACTCTTAATGCGCATGATTTCTAGAATGAGTCAAAATGTTGATATG CCCAAACTTCATGAACAAATAGGTACAAGATCATTGATGATAAATACTTTTTCTCGATGTGTACTATGTT GTGCTGAAGAAGTGGATCTTGATGAGCTTCTGGCTTCAAGATTAGTTTCTTTCCTAATGGATCATCATCA GGAAATTCTTCAAGTACCCACTTATTTACAAGCTGCAGTGGAGAAACATCTTGACTACATTAAAAAGGGC AATGTTAAAAATCATGGAGATGGACTAGTTGTTCCTTTGCCAACATATTCTTACTGTAAACAGATTAGTG CTAAAGAATTTGATGAACAGAAAATTTCAACTTCCCAAGCTGCAATTGCAGAACTTTTGGAGAATATTGT CAGAAGTAAAAGCTTGTCTCTAAAGGAGAAAAGAAGAAAATTGAAACAGAAGGAATACCCCTTGATATAT CAGAAAAGATTTCCAACCACTGAGAGTGAAGCAGCACTTTTTGATGATAAACCTACAATCAAGCAACCGA TGCTGAATTTAAGAAACCCCAAGCTTCACAGTCTGCGTTACTGACTTAAAACTTCAACAAATGAAGCCGT ACATGAGACTATTTCTACTTAAAAGAACACTGTTGATATTATAGGTTCCCTTCAAAGGACATTATACAAG GAGGTGACAAAACAGTCTTTATCTGTGCGAGGCTGCTAAAGCTACTGGATCTGCCCTAAGTGCCTAACGA AAGGTGATACCAAATATTTTAATCAGAAACTATTTTTTAATATAATGATCTGGTAAGACTATCAGAATTG TACCATCCAGATAAAATTGTGGTGCTTTTAAAGAAGAAAACACGTCTGTGTCTATATGTATATTAGCTAA AGAACTGGTTGTTTAAGATTGCTTCTTTATTTATAAGTATCAGATCATGAAAAATATTGTACACTTTTAT ATAATCTTTTTACACTTATGGTCTATTTAACTTTATATAAACACACTTGTAGTTTAGCTTTAAAAAAAAA AAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029523
• Insert Size: 2415 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023934, AAH23934
• RefSeq Size: 2946 bp
• RefSeq ORF: 2415 bp
• Locus ID: 76131
• UniProt ID: Q8CIG0
• Cytogenetics: 3 H4
• Gene Summary: May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks a two amino acid segment near the C-terminus, compared to isoform 1.