Spred1 (NM_033524) Mouse Untagged Clone

CAT#: MC202977

Spred1 (untagged) - Mouse sprouty protein with EVH-1 domain 1, related sequence (Spred1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spred1
• Synonyms: 5730461F13Rik; AL024345; AW047647; Spre
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC079606
  GCGCCGTCTCGGCTTCGCACTCGACCTCGGCGCGGCAAACCGCTGGGGTCGCCACGGCCGCGCTTGCTGC CGCCGCCCCCCTGCGGGGGTGGCCGGGCTGCCCGGCTCGCGGGGCACCGCTCCGAGTGAGGCATCCACCA TGGTGAGGCCCCCGAGCCGCTGCCCGCGCGCCCCGCCGGCCGTCGTCGCCCGCCGCCCCTCGGTGCCGCC GCTGCCGCTGCTTCCCTGCCTGCTGTTGCTCCTCCATCTCCTGATCGGATCACGGTGAGGGAAAGATGAG CGAGGAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCAGAGAT GACTCAAGTGGTGGATGGCTACCACTCGGAGGGAGCGGACTGAGCAGCGTCACTGTCTTCAGAGTCCCTC ATCAGGAAGAGAATGGCTGTGCGGACTTTTTTATCCGTGGAGAGAGACTCAGAGACAAAATGGTGGTTTT GGAATGTATGCTTAAGAAAGACCTCATCTATAATAAGGTCACTCCCACATTTCACCACTGGAAGATCGAT GACAAGAAGTTTGGCCTTACCTTTCAGAGTCCTGCTGATGCCAGGGCTTTTGATCGAGGCATTCGAAGAG CTATAGAGGATATATCTCTAGGGTGCCCAGCGTCAAAAACTGAGGCTGAAGGAGGAGATGATGATTTACA AACGACTGAAGAAGACACTTCCCGTTCCCTAGTGAAAGATCACTTTTTCCAGCAAGAGACAGTTGTTACC AGTGAACCTTACAGAAGCTCAGACATAAGACCGTTACCCTTTGAAGATCTGAATGCCAGAAGAGTCTACT TGCAAAGCCAAGTCAGCCAGATACCATTCAGTCAGCAAGGCCTAGACATTCAGAGCCGAAGTATGGAATA TGTACAGCGGCAAATATCTAAGGAATGTGGGAGCCTAAAGTCCCAAACTAGGGTCCCTTTGAAGTCAATC AGACATGTCAGCTTTCAAGATGAGGATGAGATTGTTAGAATAAATCCTCGAGATATCTTAATACGTCGAT ATGCAGACTACAGACATCCGGACATGTGGAAAAATGACTTGGAGAGAGATGATACTGACTCCAGTGTTCC ATTTTCTAAACAAGACAGTAAAAAGTCAGACTATCTCTACCACTGTGGGGATGAGACTAAGTTAAGTTCT CTGAAAGACTCTGTGGTATTTAAGACACAGCCTCCCTCGTTAAAATTTAAGTCAAAACGAAGAAAAGAGG ATGGGGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAACGCCAGGGGGAAATG CCAGGACGCTCCAGATCCTGTTAAAAGATGCATATATCAAGTTAGCTGCATGCTCTGTGCTGAGAGCATG CTATATCATTGTATGTCAGACTCAGAGGGAGACTTTTCTGATCCTTGTTCGTGTGACACTAGCGATGACA AGTTCTGCCTAAGGTGGTTAGCCCTGGTAGCTCTGTCTTTCATTGTACCATGTATGTGCTGCTACGTCCC TTTGAGAATGTGCCATCGCTGTGGTGAGGCATGCGGGTGCTGTGGTGGGAAACATAAGGCTGCTGGGTGA AGCAGTCCAGGGCCGGCATGAGCTTAAACCTTCATCTCCAGGAATTAGCTAACTTGGATTTGTGGAAGCT GTTGGCAAGCAATATGGAATCTTGCCGGTATCATTGGGGCCATGCGTGGAGGAAGCAGAACTCATCAGTT CTTGTGATTTACAGATGCTAGCCGTGCCTGTTTCCCTGAGTGCATGGCATGTTCTGGGACAGATCACAGA GCATTTTCAGAAGGCAGTCATTTCTGGTTATTGATTACAAAAGGTCAACATAGAATAGGATCCAACTAAA AGGGATTAATTTTGGCATTTTCATATTTATGCACTAGGTGATGGGACTTTTTAAAGATTTGAATTCTTTA GGACATTAACTAAAAAGTGCACTAAGGGACAGTGATTTCAGTACAGAAAAGTTAGTACTTGGGAAATTAA GAGATAAGACAAGTGCAACTAAATCATTGGTTGTTTTTTGAAAGCAGTTTTATGTATAAATAACAAATGT TTATATTTAACTAAATGTAAGGTACGAACTATGACATATTAAACTTTTCTTGCCCTTCCTAGTTCTGAGG TAGATGTGCACATATCTGCCACCACATTCCATATGTGTTGAGTAGTTAACTCATCTAGTTAATAACGTTT GTGTCCATGTGAGAGATTCGGTGAGTTCATCCTCAGCTCTCTTGGGCTGCAGTTTATATTGAGTCATATC TGTACATTCTGGAGATCTAAAATCTTTAAAATACTCTAATAGCCTTGAGTGACCAACCTTTTTTTTTAAA GCACAGATGTAATTGTCTAATGTTCTGATGGAAATGTAACACTTATTTTTATATAAAGAGATTGAGTAAA CAGAAGAAATTGAGGGTTCCTTTAGTTGTTTTGTTTCTGTGGTATTCAGCCAGCAAGTTGTTTTCTTTCA GAGTTTCCTCCTTCAGAGAACTTAACTGCATTTATAAACGTTTTACAAGGCAGAAGCATTAACTGGATAG TTAGTGTGAAACAGTTTCCGCTTGAGATCTCCATTTCTCACTCTGCTGAGCCAGAACTCGCACAGCTGTT TCACTGATTTTCTGTGCTTATTACTCACATAACAATGGTAATTAATACCTTTCAGTTGCATTTTATTATA TTTCAGGAGCAAAGTTACTTTGCAGTAACACATATGCAGAATTCTCCCAGGAAAGTATTCAATCCACCAT TGTGAATGGAAATCTTATACACCACATTCCTGTCGCAGAGCATTTGTATTATGGGATGTTTGATTTACAA AGTAGTGTGATATGCTCCAGCAAACAGGTTAAAAATACTTTGAGGTCAAATTGTCTTGGTTTTGTTTTTT TAAAGTGTTACTATTAAAACTCTTGTGAGGGATTGTTGCAAAATACTCCCTCAGGAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_033524
• Insert Size: 1335 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079606, AAH79606
• RefSeq Size: 3042 bp
• RefSeq ORF: 1335 bp
• Locus ID: 114715
• UniProt ID: Q924S8
• Cytogenetics: 2 E5
• Gene Summary: This gene encodes a membrane-associated protein that is phosphorylated by tyrosine kinases in response to growth factors. The encoded protein acts as a negative regulator of the mitogen-activated protein (MAP) kinase signaling pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.