Ndufv1 (NM_133666) Mouse Untagged Clone

CAT#: MC202923

Ndufv1 (untagged) - Mouse NADH dehydrogenase (ubiquinone) flavoprotein 1 (Ndufv1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ndufv1
• Synonyms: CI-51kD
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC014818 sequence for NM_133666
  CGCACTTCCTGTGCGCTCCCCACCCCGTGTGTTTCTGTCGCGCCGGTCCCGCGGTTCGGAGTTGTGAAGG TGACAGCCGGAGGTGACCCGTCTGGCTCCCGCGATGCTAGCGGCACGGCATTTTCTCGGCGGGTTGGTTC CCGTGCGGGTATCTGTGCGTTTCAGCAGCGGCACGACAGCACCCAAGAAAACCTCATTTGGCTCACTGAA GGATGAAGACCGGATCTTTACCAACCTGTATGGGCGCCATGACTGGAGGCTGAAAGGTGCCCTGAGACGG GGTGACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGCCTGACTGGATCTTGGGTGAGATGAAGACAT CAGGTTTACGGGGCCGTGGTGGTGCTGGCTTCCCCACTGGCCTCAAGTGGAGCTTCATGAATAAGCCCTC AGATGGCAGGCCCAAGTATCTGGTGGTGAATGCTGACGAGGGAGAGCCAGGCACCTGTAAGGACCGAGAG ATCATGCGTCATGACCCTCACAAGCTGGTGGAAGGCTGCCTTGTGGGAGGCCGGGCCATGGGAGCCCGGG CCGCCTATATCTACATCCGAGGGGAATTCTACAACGAGGCCTCCAATTTGCAGGTAGCTATCCGAGAGGC CTATGAAGCAGGTCTGATTGGCAAGAATGCTTGTGGCTCCGACTATGATTTTGATGTGTTTGTGGTGCGT GGGGCTGGGGCCTACATCTGTGGAGAAGAGACGGCACTTATTGAATCCATTGAAGGCAAGCAGGGAAAGC CACGCCTGAAGCCGCCCTTTCCAGCAGATGTGGGAGTGTTTGGATGCCCCACAACTGTGGCCAATGTGGA GACAGTGGCTGTGTCCCCCACCATTTGCCGTCGTGGGGGCACCTGGTTTGCTGGCTTTGGCCGAGAACGC AATTCAGGTACCAAATTGTTTAACATCTCTGGCCATGTCAACCACCCCTGCACTGTGGAGGAAGAGATGT CTGTGCCACTGAAAGAGCTGATTGAGAAACATGCTGGTGGTGTCACAGGTGGCTGGGACAACCTCCTTGC TGTGATTCCTGGTGGCTCATCTACTCCACTGATTCCCAAATCTGTGTGTGAGACCGTGCTAATGGACTTC GATGCACTGGTGCAGGCTCAGACAGGCCTGGGCACGGCTGCAGTTATTGTTATGGATCGCTCGACAGACA TTGTGAAAGCCATCGCTCGTCTCATTGAGTTCTACAAGCATGAGAGCTGTGGCCAGTGTACCCCGTGCCG TGAGGGCGTTGACTGGATGAACAAGGTGATGGCCCGATTTGTGAAGGGAGATGCCCGGCCAGCTGAGATT GACTCCCTGTGGGAGATCAGCAAGCAGATAGAAGGCCACACCATTTGTGCTCTGGGTGATGGGGCTGCCT GGCCAGTACAGGGTCTGATCCGACATTTCAGGCCAGAGCTTGAGGATCGGATGCAACGGTTTGCCCAGCA GCACCGGGCCTGGCAGGCAGCCTCGTGAGTCCACACCCTAGCCTGACAATTTCTCATCCCTTTGGGAAAC TGGACAATAAACAAGTGCTGTCCACTCTCCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133666
• Insert Size: 1395 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014818, AAH14818
• RefSeq Size: 1585 bp
• RefSeq ORF: 1395 bp
• Locus ID: 17995
• UniProt ID: Q91YT0
• Cytogenetics: 19 A
• Gene Summary: This gene encodes a subunit of the NADH-ubiquinone oxidoreductase (complex I) enzyme, which is a large, multimeric protein. It is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. This gene is a core subunit and is conserved in prokaryotes and eukaryotes. The human ortholog of this protein has been characterized. It has consensus motifs for NADH, flavin mononucleotide, and iron-sulfur binding sites and participates in the oxidation of NADH as part of the dehydrogenase module of complex I. In humans, deficiencies in complex I are associated with myopathies, encephalomyopathies, and neurodegenerative disorders. [provided by RefSeq, Jun 2013]