Gcsh (NM_026572) Mouse Untagged Clone

CAT#: MC202905

Gcsh (untagged) - Mouse glycine cleavage system protein H (aminomethyl carrier) (Gcsh), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gcsh
• Synonyms: 1100001L02Rik; 5730591C18Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC014745 sequence for NM_026572
  GCGCAGTTACCCTCCGGACCTCTGACCATGTCGCTGCAAGTGTCGAGGAGCTTGCGGGTTGTGGCCTACA GCCTGCGCACCGCCCTGGCCTTCTGCTCGCCGCGGCCCTGTGTACCGAGCGCCGCCGCCGTCCGCTCACT GCGCACCGGATCTGCTTTGCTGTCGGTGCGTAAATTCACAGAGAAACATGAGTGGATAACGACAGAGGAA GGTATTGGAACGGTGGGAATCAGCAATTTTGCACAGGAAGCGTTGGGAGATGTTGTTTACTGTAGTCTGC CTGAAGTTGGGACAAAATTGAAAAAACAAGAGGAGTTTGGTGCTTTGGAGAGTGTGAAGGCTGCCAGTGA ACTCTACTCTCCTCTGTCAGGAGAGGTAACCGAAGTCAATGAAGCTCTTGCCGAAAACCCAGGGCTTGTC AACAAATCCTGTTACGAAGATGGTTGGCTGATCAAGATGACACTGAGTGACCCTTCAGAGCTGGATGAAT TGATGAGTGAAGAAGCATATGAGAAATATGTGAAGTCCATTGAGGAGTGACCATGGCACCCCTCTACTCC CAGATCAAACTGGATGTGACCTGCTCCAGCATGCTTGTCTTAAACTAGTGGAGAATAGAGGACTTGAAAT AACTTTTAGCAATACTGATGGAAAGAAACTGTTCTTGACAGTGCTACTTAAGACCCACCCCCAACTTCCT AAAGGATGCAGATAAACACAGTGTACAGGTTTTTGATAATTGCCATACTAAGTATTTAGACTAGGGGTTA AGCCCTGGTGTTTAGAATTCTTAGGATCGTCTGCTGTGAAACGTGGTTATGGGGCTGGTTTTGGTGATGG ACCACCTTCTAATCCCAGCACTCAACAGGCAGATGTTAGTTCAAGGTCGGTCTACACAACAAGTTCTAGA CCAGCCACAGCTACATGGCGAGACCCTACCTACTGGGTATAAGGCTTTCATAATTCAAGATAATAGTGGT GTATCTTAGATAATTTTGTTACTTGCCCATATCCACCACTGGATTTGGGTCAGAGACTTAGTGTCTTCTC ATTGGGAATGCAAGGAAGTGTTGTACAGTGTCAGAGGCAGGAGAGTACTGTCTTTGTTTCACCATGTGCT GTGTTAGCTGTTGTTTTCCCACCGTGAAGCACCGGCTTTGCAACACTGAGAAACAAATAAAACATTCATC CTCCTTGTTAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026572
• Insert Size: 513 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014745, AAH14745
• RefSeq Size: 1220 bp
• RefSeq ORF: 513 bp
• Locus ID: 68133
• UniProt ID: Q91WK5
• Cytogenetics: 8 E1
• Gene Summary: The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST) (By similarity).[UniProtKB/Swiss-Prot Function]