Kng1 (NM_023125) Mouse Untagged Clone

CAT#: MC202872

Kng1 (untagged) - Mouse kininogen 1 (Kng1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kng1
• Synonyms: Kng
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC018158 sequence for NM_023125
  GAGAGATCAGAGCCCAGAGGCTGCCAAAATCAGCTTCTGAGTTGGTTTCCCATTCTTGGCAAAACAGTCC CTCAGTTCTAGAGGGACCCTTAAATCATGAAGCTCATTACTACACTGCTCCTCTGCTCCGGACTCCTGCT GACTTTAACACAGGGAGAAGAAGCGCAGGAAATTGACTGCAATGATGAGGCTGTATTTCAGGCTGTGGAT TTCTCTCTGAAGCAGTTTAACCCTGGGGTAAAAAGTGGCAACCAGTATATGTTGCACCGAGTGATCGAGG GCACTAAAACGGATGGCTCTCCAACCTTTTACTCCTTCAAGTATCTAATCAAGGAGGGCAACTGCTCTGC TCAGAGTGGCCTCGCATGGCAGGACTGTGACTTCAAGGACGCTGAGGAAGCCGCCACTGGAGAATGCACA GCAACTGTGGGGAAAAGAGAAAATGAATTCTTCATAGTCACCCAGACCTGCAAGATTGCTCCAAGTAAGG CCCCCATACTGAAAGCCTATTTCCCCTGTATTGGTTGTGTGCATGCCATATCGACAGATAGTCCAGACCT GGAGCCTGTTCTGAAACACTCCATCGAACATTTCAACAACAACACAGATCACAGCCACCTCTTTACTCTC AGAAAAGTAAAAAGTGCCCACAGACAGGTGGTGGCTGGCCTGAATTTTGACATTACCTACACAATTGTGC AAACAAATTGTTCAAAGGAGCGTTTTCCTTCCCTCCATGGAGACTGCGTGGCCCTTCCCAATGGTGATGA TGGTGAATGTAGAGGAAATCTCTTCATGGATATTAATAACAAAATTGCCAACTTCTCACAGAGCTGTACC CTTTATTCAGGAGATGATTTGGTAGAAGCGCTTCCCAAGCCTTGCCCTGGCTGCCCCAGGGACATACCTG TAGACAGCCCAGAGCTGAAGGAGGTGCTTGGTCATTCCATTGCACAGCTAAATGCAGAGAATGACCATCC TTTCTATTACAAGATTGACACCGTGAAAAAAGCAACATCACAGGTGGTAGCAGGAACTAAATATGTTATT GAGTTCATAGCCAGAGAAACCAAATGCTCCAAGGAAAGTAACACAGAGCTGGCAGAAGATTGTGAGATCA AGCACCTTGGACAAAGTCTCGACTGCAATGCTAACGTGTACATGAGACCTTGGGAGAACAAAGTCGTCCC GACTGTGAAATGCCAAGCATTAGATATGACTGAAATGGCAAGAAGGCCTCCAGGTTTTTCTCCTTTCCGG AGTGTCACAGTACAAGAAACAAAAGAAGGAAGAACTAGGCTCCTACGCGCGTGCGAGTACAAGGGCAGAC TCTCAAAGGCAGGGGCAGAGCCAGCGCCTGAGCGTCAGGCAGAATCTTCACAAGTGAAGCAGTAGTCCCA GCAATGACCCAGAGGGAAGGACCAGAAGAATCCTGGGATGTGTGGAGCGCGGGACCATCGTCTTCATCAC CCTGATCCTAGTGGAAATAAAATTCAGACTTGATGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023125
• Insert Size: 1299 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018158, AAH18158
• RefSeq Size: 1520 bp
• RefSeq ORF: 1299 bp
• Locus ID: 16644
• UniProt ID: O08677
• Cytogenetics: 16 B1
• Gene Summary: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region, compared to variant 1. The resulting isoform (2), also known as LMW kininogen-I, has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.