Sgpl1 (NM_009163) Mouse Untagged Clone

CAT#: MC202842

Sgpl1 (untagged) - Mouse sphingosine phosphate lyase 1 (Sgpl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgpl1
• Synonyms: AI428538; D10Xrf456; S1PL; Spl
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026135 sequence for NM_009163
  CCACGCGTTCCGACCGAGGCGCGTGAGGTGGGCGGCGGCCAGCGGAGCCTCTGCGGGGAAAGAAGGAGCC GGGTCCACAGCCTGAGGAGACGCAGAGGCAGGGCAGCCCCGGGGAGGGAGCCGGCTGCAGAGGAAGATGC CCGGAACCGACCTCCTCAAGCTGAAGGACTTCGAGCCTTATTTGGAGATTTTGGAATCATATTCCACAAA AGCCAAGAATTATGTGAATGGATATTGCACCAAATATGAGCCCTGGCAGCTCATTGCGTGGAGTGTCCTG TGTACTCTGCTGATAGTCTGGGTGTATGAGCTTATCTTCCAGCCAGAGAGTTTATGGTCTCGGTTTAAAA AAAAATTATTTAAGCTTATCAGGAAGATGCCATTTATTGGACGTAAGATCGAACAACAGGTGAGCAAAGC CAAGAAGGATCTTGTCAAGAACATGCCATTCCTAAAGGTGGACAAGGATTATGTGAAAACTCTGCCTGCT CAGGGTATGGGCACAGCTGAGGTTCTGGAGAGACTCAAGGAGTACAGCTCCATGGATGGTTCCTGGCAAG AAGGGAAAGCCTCAGGAGCTGTGTACAATGGGGAACCGAAGCTCACGGAGCTGCTGGTGCAGGCTTATGG AGAATTCACGTGGAGCAATCCACTGCATCCAGATATCTTCCCTGGATTGCGGAAGTTAGAGGCAGAAATC GTTAGGATGACTTGTTCCCTCTTCAATGGGGGACCAGATTCCTGTGGATGTGTGACTTCTGGGGGAACGG AAAGCATCCTGATGGCCTGCAAAGCTTACCGGGACTTGGCGTTAGAGAAGGGGATCAAAACTCCAGAAAT TGTGGCTCCCGAGAGTGCCCATGCTGCATTCGACAAAGCAGCTCATTATTTTGGGATGAAGATTGTCCGA GTTGCACTGAAAAAGAACATGGAGGTGGATGTGCAGGCAATGAAGAGAGCCATCTCCAGGAACACAGCTA TGCTGGTCTGTTCTACCCCACAGTTTCCTCATGGTGTGATGGATCCTGTCCCCGAAGTGGCCAAGTTGGC TGTCAGATATAAAATCCCACTCCATGTGGATGCTTGTCTGGGGGGCTTCCTCATTGTCTTCATGGAGAAA GCAGGGTACCCACTGGAGAAACCATTTGATTTCCGGGTGAAAGGTGTGACCAGCATTTCAGCAGATACTC ATAAGTATGGCTATGCTCCTAAAGGTTCATCAGTGGTGATGTACTCTAACGAGAAGTACAGGACGTACCA GTTCTTTGTTGGTGCAGACTGGCAAGGTGGTGTCTACGCATCTCCAAGCATAGCTGGCTCACGGCCTGGT GGCATCATTGCAGCCTGTTGGGCCGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACCAAAC AGATCATCAAAACTGCTCGCTTCCTGAAGTCAGAACTGGAAAACATCAAAAACATCTTCATTTTCGGTGA TCCTCAATTGTCAGTTATTGCTCTGGGATCCAACGATTTTGACATTTACCGACTATCTAATATGATGTCT GCTAAGGGGTGGAATTTTAACTACCTGCAGTTCCCAAGAAGCATTCATTTCTGCATTACGTTAGTACATA CTCGGAAGCGAGTGGCGATCCAGTTCCTAAAGGATATCCGGGAATCAGTCACACAAATCATGAAGAATCC TAAAGCTAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGGCAACCATTGACAGGAAGCTGGTT GCAGAAATATCCTCCGTCTTCTTGGACTGCCTTTATACTACGGACCCCGTGACTCAGGGCAACCAGATGA ACGGTTCTCCAAAGCCCCGCTGAACTTTGACCCTTGTGAGTTCCTTGGAGCTTCAGGCCTTCAGAAGGTT CTTGGCGTTTGGAACAGACTGCACACAATTTGACATCTGGACTGGCTCCGTAGAGCACAATAGGATAAAC CATAAGATAGCTTCAGCCTCAGGACTCCTTTGTTTCCTTGTGGGGCTTTTAGTTTGAAGACCCTGAAGGA TCCCACTGCATAGTGAGTCTGTCCTGGTTATTAATGTTACCCTAGAAATTGCTTTAACCTTATTTTTCTC TAACGTCTTTAGCATCGTCTTTACTTAATCATTGTGTGGTGGCTCCGACCTGTCCTGATTATTTAGGGAA AGTGTGAGATAGCAAGAGGGTGTCTCTGCTGCCTCAGTGGGGTGTTCTGGCCATCAGGCAGGAAGCAGTT ATGTACCGTGTTCCCTCAGGTGGGTGTCTGTCTGTCCCACGCAGGGGGAGGTTCAGCCTCTGCTTCAGGA GATGCCTCTTGTCCTCAGGATCACTGTCATTACAGCTTGCTTTACTTTTCCAAGCACACGTCGGCCAGAC TACTGGCCTCTTTTTTTGTTTTATTTTTAAATAACTGGAGTATATTCCCTTGGCAGGGTTTGAAGTGCTC CTTTGTCTTGTGCATGACCATGGCTGCTGCCATCTTTGGAGATGCCTGTTGGCAGTCATATACTTGTTTG TTCTTATTACTGTAGGGTAAGAAATGAACATGTTTTTCAGAGAATCTAGGGCTTTCTGGCTGTTGGCACT GCCTCTCTGGATGGAGTAGGGGTAGATCACTCCTGAGCCCTCTCCTGGGCCAGAGGAGCCACAACCAGAC CCTTTCCCACATTTTCGTGAATAGCCACATAGGTAAGCGCCTACATCCTGCACATGTGGGCAGTAGTCTT CCCCTTGGACATTGCCTTAAGCCTTCATGTAATGAGAATCTGCTTTTATTGAAGAAAACACTCCACAAGT GTCCTTGACTTCCTCATTGGGTGTGAAGATGTGCAGAGGACACTTGCTCCATCAGTGTGTGTTCTTCCTG CCCCTTCCCCCCTTATTCAGCTTCCAAAGGTACGACAGCATTTTAGCTTCAGGTACTGGACGTTCCTCAG GCCCCGCTAAAAGGAAGCAGACATTTTGGAGTAGAAAGGGATGGCTACAGCCATTACACTTGGGGCCTGG GGTTCCTTCTTAAGACTGATTGCCCAGGGCTATACAGGGACAGGACCAATCCCTGGCCAATTTCCCATTT GCTTCTCCCTTTTAGGGATTGGGGGCTCTGAAGTAGGCAGAGCCCCGTTCAGCTCTGATGACCGTACCAT GTGCCTTTCTACCCATTCAGTGAGTGACAACCCTCGTCCTCACCCAGGCCCTGGCAGAAGTAGACCACTC TTTGAGGTGTGTCTGCTTATAGGAGAGAACTTGGGACAGATCACCAGAAGCCATGCTCTGTTCCTTCCCC TTCTCAGGGATTCCTGAGTATTCAGCCTGGGCATTGCTCGCTGGGAGAGCCAGACCACCAGGCTCTTCCT CACCCTCCTTAGGAAGTGGTCTTCAGCACTGTTCTGACCCCAGGTGTTACCAGCTGGTTCATCACAGGGC ATGTGTATATGTCCACTTCCCTCCTGTATGCTCTACAGTGCATTATGAGGCTCAGGGATGGTTGGTGTGT CAGAGAGAGGCTGAATTCCTGAGTGTACACATTAACAGTGGTTAGACTGTTGGACTCCTCTGGGCCTCCT CTCTGGCTTACAAGTGGCTAAAATGGAGAAGCATCCTTTATAGTCTCAGCAGAGCCAGGGACCAAGCCGC TCCCTTCCTTTGTGGTAGCACTAGCATGGGGTGCTGGTGTTTTTCAGAGGGACCCGCTGCAGCCATTTTA ATTCATGATTAGGAACCTTAGTGTGACTTAAGAGTTTCATTGTGTGTTTATGTGTGCATGACATGCCTAG TCAGTGACAGGGGTGGGTAGGGTGTCTAGTATCATGGTATCACGTGGCTTAGTAGATGCCACAGTAGCTT CTGCAAACACTGTACTGTCTTGTTTCTGTATTAGATGTCGGTAATCATGTGAATATACTGCCATGTCACT TTTAATATTACCAGTTTTATACTTGGAAAATGGTACTTGCCTCTTTGAAATCTATTTTGTCTCTAATGTC CCTACCATCTCTATGTTCTTTCCTAACTGCAGCAATAACCCTTCAAAAGCAATTGAATAATAAATGTCCC AAACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009163
• Insert Size: 1707 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026135, AAH26135
• RefSeq Size: 4108 bp
• RefSeq ORF: 1707 bp
• Locus ID: 20397
• UniProt ID: Q8R0X7
• Cytogenetics: 10 32.14 cM
• Gene Summary: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (PubMed:9464245, PubMed:20097939). Elevates stress-induced ceramide production and apoptosis (PubMed:9464245). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts (PubMed:20097939, PubMed:28262793). Involved in the regulation of pro-inflammatory response and neutrophil trafficking (PubMed:21173151). Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (PubMed:28521611). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein.