Eya3 (NM_010166) Mouse Untagged Clone

CAT#: MC202813

Eya3 (untagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eya3
• Synonyms: AI844637
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC063259 sequence for NM_010166
  CGTCCCATGTCGTGCTGGGCAGTGTCTGCAGCCGTCTGTTTGTGAAGACCGCAGAGACCTGCAATTCAGT CCCCTAAGTGTGTTAGGAGATAGTCGTCCTCATGGAAGAAGAGCAAGACCTACCAGAGCAACCGGTGAAA AAAGCCAAGATGCAGGAACCAAGAGAACAGACTTTAAGTCAAGTAAACAACCCAGATGCCAGTGATGAGA AGCCTGAGACATCCAGCCTTGCGTCAAATCTCAGCATGTCAGAGGAAATTATGACATGCACCGATTACAT CCCTCGCTCATCCAATGATTATACCTCACAAATGTATTCTGCAAAACCTTATGCACACATCCTCTCAGTT CCTGTTTCGGAAACCACTTATCCTGGGCAGACTCAGTACCAGACACTGCAGCAATCTCAACCCTACGCTG TCTACCCTCAGGCAACCCAAACTTACGGACTACCTCCTTTCGCTTCAAGCACAAATGCCAGCCTGATACC CACTTCATCTGCAATTGCCAATATTCCAGCAGCAGCTGTGGCCAGCATCTCAAACCAGGATTATCCCACC TATACTATTCTTGGACAGAATCAGTACCAGGCCTGCTACCCCAGTTCCAGCTTTGGAGTCACAGGTCAGA CTAACAGTGATGCTGAGACCACAACATTAGCAGCTACAACATACCAGACGGAGAAGCCTAGTGCTATGGT GCCTGCACCAGCCACACAGAGGCTTCCCTCCGACTCCTCTGCAAGCCCACCTTTGTCCCAGACTACACCA AATAAAGATGCTGATGATCAGGCCAGGAAAAACATGACTGTCAAGAACCGGGGCAAGAGGAAAGCTGATG CCAGCTCTTCCCAGGACAGTGAATTGGAACGGGTATTTCTCTGGGACTTGGACGAAACCATCATCATCTT TCATTCCCTTCTCACTGGATCCTATGCTCAGAAGTATGGAAAGGACCCAACAGTAGTAATTGGCTCAGGT TTAACCATGGAAGAAATGATTTTTGAAGTGGCTGATACACATCTATTTTTCAATGACTTAGAGGAGTGTG ACCAGGTGCATGTGGAAGATGTGGCTTCTGATGACAATGGCCAGGATTTGAGCAACTACAGTTTCTCCAC AGATGGTTTCAGTGGTTCAGGAGGCAGTGGTAGCCACGGCTCATCTGTGGGCGTTCAGGGAGGTGTGGAC TGGATGAGGAAACTGGCCTTTCGCTACCGAAAAGTGAGGGAAATCTACGACAAGCATAAAAGCAATGTGG GTGGCCTCCTCAGCCCCCAGAGGAAGGAAGCACTGCAGAGACTCAGAGCAGAGATCGAGGTGCTGACGGA CTCCTGGTTAGGAACTGCGCTCAAGTCCTTGCTTCTCATCCAGTCTCGAAAGAACTGTGCGAATGTTCTG ATCACTACCACGCAGTTGGTTCCAGCCCTGGCCAAGGTTCTCCTGTATGGACTAGGAGAGATATTTCCTA TTGAAAACATCTACAGTGCTACCAAAATCGGTAAGGAGAGCTGCTTTGAGAGAATTGTTTCGAGGTTTGG GAAAAAAGTCACATATGTAGTGATTGGAGATGGACGAGATGAAGAAATTGCAGCCAAGCAGCACAACATG CCCTTCTGGAGGATCACAAACCACGGAGATCTGGTGTCCCTGCACCAGGCTTTAGAGCTTGACTTCCTCT GAGAACTGGAATGTGGACCCTTCCTTCCTTGTGAGCTTCTCTTTACCTCCAACAGGAGCCAGAAGCCAGA ACCCTCTGAGCCCCTTCTCTCCTGTCTGTCTCTCGGGTCTCAGTGCCCCCTCCCCCTTCTCTTCTGCTCT TTCTCCCTCCATGAAATGCTGGCGAGAACACAATCAGAACCAACAACTGCAGTTATTCTGAGTGAGCTGC AGCCCATGTCCCCTGTACAGCAAGAGGTGGCTGGATAGAGCTGCAGACCGGCTGCCGCTACCGTGCTTTA ATTTTTCTGTTTCAATTGAAAAAGGAAGAACAAGAAAAGCCGATGCTTGGGGCACAGTTGTACTCTTGCT GCATGACGGACTGACCGGGAGCTGCTCCTGATGTGGATGGAAACTGTCCCCTTGAGATCGTCTTCTGGTC TCTTAGCTATAGAATGTCTCTCCCAGTGAATGGGTATGTTATTTTTATAGGTGAGGGTCTGGTCTCTACA GCAGCCTCCCCCACTTTTCTATGAAGAAAGCCGTGTGTAAAGTTTCCGTGACAGTAGTAATGGAAATATC TAAAATACCTCCGTGACTGGACAGGACAGAGGCTCCAGTGCTCTGCTCCCTGGGGAGGCTCCTTGCCATC TACAAGGCCCTGGTCACTTACTTTAGACTGTGGCATCTCCCATCTTGTAAGCCTCGGTCTCCTTGGCAGT CCTCCTTCATCTGTAACTAAATGGCACAACCCTGGAAACTCCTTCCCATAGACAATAAATCTAAGCCTTT AGCTTGTACTTCAGAAGTTCTGTCCCAGGAGGCTCAGCCTCCAGACTGGAGAGAAGGGCTCAGATTTCTT AGGACTTTCACCTCGTGCTCCTGCAGAGCAGTACCTGTTCCCAGCAGATGCTTCCTCTGTGGTCGGCTCA GTCGTCAGATGCTGGCCTCACGTCCTGTCCTCACAGCTACCTGTCTCTGTGCAGCTCTGGCCAGCCCCTA CCCACTCATTGCAAGTCAGAAAGGCCAAGGGGGCAGGCTCTAGCTGCCTCCTTCACCTGCACTTACATGC GGTGATCCCACCTTGTATTTATACAGATCTCTGCCTACGAGTGGAGAGCAGAGAGCTAGAGTCAAAACCC ATGAAATATCCCACTGCTTAGAGTCCATGCTGAAGTGGGAAACCAGGCCAGAAAGACTTTCTTGACTTAA GTGCTTAAAAATACAATCCTGAGCTAGGTGCAAGAGCACCTCTCAAGGCTGCCCCTAGTCAGTCAGGAGG GAAACTGGGCCTTAGTGCCAGCCCGCAAGCCCAGCTGTGTTTTGGTTTTATTTTGGTTTTTGTTTGGGTT TTTTGTTTGTTTTTTACGGCACTATCCCTTCAGGACCAATGTCGGTGCCTGTTTAATTGGGTTAAACGTC CCTAGAGTTGAGGAAATGTGCAGCCTTGGTCTTCAGGAGGTCCTTGACCAACCCCTAGCTATAGTCACTG TCCGCAGCGATACCACAGCCCAGAGGCAAAATTCCTCTTCATGCTAGGCTACCAGATAACGACAGAAAGG CTGACACCACCTTCAGAACTGTTGGACACTTCTGAGTACTTCTGAGACCAGCAGTTGAGGAAGGGGCTCA GACAGGAGGTGAGGTTTGTGTCTTGGCCATTCATTTGGCTTTGTTGTGAGGTACTGGGTGGGAGCAAGGA GCTAGAGTCTAAGTCAGACCCTCCTAGGCTGCCATTAGCACCACACCGCTACCCTAGTCTGGCCAGCCTT GCTTGGTGGTCCAACCTGGGAAACTCCAGGATGAGTTTTACTTCCAAGTCTCAGTCCTTCCCTCATTAAC AGTGGAGTTTCTTGTGCTGGACAACGCCACCTATTAGTGCTGTGCTTCAGGCTCACCAGACTGCCAAGTG GCGACACACCCTTCTGCACACAGGTACCACCACAAACACTTCAGGGGAGACTCTTCTTAGAATATAACAG GTAATCCCTTTCCTCTTGCCCTGACTGGAGAGGCAAGGAGTGTTCTGAGCTGAGTGGCTACTGTTCCCAA AGCAGCTACCCTGTCAGGGTAGACAGAGGAGTGCATTGGTCTCTCCAGGGACTGCTGCCTTTGTGCTGAC TCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCACCCACCCACAGAGGCTTGGGAAGGAAAATGAGC AGGCAGCATCAAGAGCTGCTAGTAAGAGAAAGACAGCTTGGTGGTAATAGCACCCGCAGCTTGTGCAGAG GTCCAAGGTTTGAGCACCCACAAGGCTATTCACAACCTCTAGCTGCAGCTCCAGGGGGTTCGTTGCCCTC TTCTGGCCTCTTCAGGCACCAAGCACTTATATACGTGTAAGAAACACTCATAAAATAGAAATTCCTTTTA ATATTAAAAGCTGTTGGAAGTTAGAAGGCAGTCCTTACTGAAGGAACACCTTGAAGAAATTAGCTGGTGA ATCTTTTCCCTGCTGGATCCTGTGTTCTGGAAGATTAGTCAGGACATCTCATGGTGACAGCTAGTTCTAG GAGTGTGTGGGGTGGCAGGAATGTCCTGGGATACAAGGTGGCTTGACTTTCGAATGTAAAAGTGACTGTG CCTTAGCATCACCCTGTCCAGTGCCCCTGGGGCAGCCGGTGATGTCCGCGAGAGTGGATACCTGCTCCAC ATTTTAGGTGCCTACTTGGGCTTTTGATTTGGGGGTGTTCTCTTCTCTGCTAGTCGGGTGACAGCTAGTG TCCCAAGAGACTTGGCACAGCTTGTCCGTGTTCACCCAAGCTCAACCTTCACAGCTCCAAGTAGAATCTG ACTGGCAAGCTGCAGCAGCAAGCTCATGGCGCTTCCATAGACACTGCTTTGAATGGGAGTCACACAACTG ACAGGAGCACCAGGCAGAGCTCGGTGCTGTCCTGGAAGAGTGCAGGCTAGCCGCCAAGTGCCAAGCTTGG GCCTCATTCCTCACTCTCCCCAGGTAGCTGAGGCCCTTGAGTAAGCGTGTGCTCCCGGCTGCTCACTGGT CAAGCAGCTAGAACTACCCAGACACACCCATTGTAGATGCCAGGTTGGCGTTCTGAGCTTCCCAAACATC TAAACCACGAGGGGGAGAACCCCGCTTGTGTCCCAGCCTCATCTGCGGGCCGCTTGCCCTCCCTCTACCC TGAGTCAATTCTAGTGAGCATGGCTCCCTGGCTGCCTCCTCACACTGAGCGGAGCCATCCCAGTTCAGGA CCATTTCTACTTGGATCTCCACTCTCCCTCTGGACTCCAGATGCCTCTGTAGCAGGAAAAGGCATCCATG GGTGGGATATATATATTTATGTGACTGCGGGCATTTGTGGCTATAGAGTCCTTGACCATAATGTTATATG TAATGGGAACTATCTTATAAACTTGAAAAAATAAAGTTTTTATTTTCTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_010166
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063259, AAH63259
• RefSeq Size: 5108 bp
• RefSeq ORF: 1581 bp
• Locus ID: 14050
• UniProt ID: P97480
• Cytogenetics: 4 65.68 cM
• Gene Summary: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (By similarity). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. The phosphatase activity has been shown in vitro. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye. May play a role in mediating the induction and differentiation of cranial placodes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2 which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.