Mterf1a (NM_001013023) Mouse Untagged Clone

CAT#: MC202791

Mterf (untagged) - Mouse mitochondrial transcription termination factor (Mterf), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mterf1a
• Synonyms: 4931431L11Rik; 9230106K09Rik; Mterf; Mterf1; mTERF1b
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC082778 sequence for NM_001013023
  GGGCGGAAGTGAAAGGTGTCTACCGCGAGTGTTGCATTTGAAAAACTTTGGGCTACCTGATGATTATGGC ATCAAGAAACATCTGGTGTGTGAGAAGAAACTTTCTCTTTGATTTAAGAGGCTGGATGCTTCAATATTCA GCAGAAGTTTTCCTCAAGTCGATTTCATTCAGGCCGTTTAGTGTGGAATGCGACAGTAAGGACAAGGAGT CTTTGGAGGAGGAGAGGGAGGACCTGCTGAGCAACTTAGTCACCATGGGCGTTGATATCGACATGGCAAG GAGGCGACAGCCTGGAGTTTTTAACAAGGCTGTTACGAATGAGCAGGAGCTGAAGATATTTCTTCTGTCC AAGGGAGCTAGTGACAAAGTGATTGGTAGCATCATATCAAGATATCCACGAGCTATAACACGCACTCCTG AAAGTCTTTCAAAACGGTGGCATCTGTGGAGAAAGATTATGGCATCAGACCTTGAAATTGTAAATATTTT GGAGCGTTCTCCTGAATCCTTTTTTCGATCTAATAACAATCTAAACTTAGAGAATAATATAAAGTTCCTT TGCTCTGTTGGATTGACTCATAAATGCCTCTGCCGACTGTTGACCAATGCTCCCAGAACCTTCTCCAACA GTCTGAATTTGAATAAGCAAATGGTTGAATTTTTGCAGGAGACTGGTATGTCCTTAGGTCACAATGATCC CAGAGATTTTGTCAGAAAGATAATTTCTAAAAACCCTTCCATCTTAATTCAGAGCACCAAGCGTGTAAAA ACTAACATTGAATTTTTACAATCAACTTTCAACTTGAACAAACGGGACCTGCTGCTTCTGATATGTGGCC CAGGAGCTAGAATCTTAGACCTTTCCAATGACTGTACCAAAAAAAATTACACAAACATAAGAGAGAGGCT GCTTTCTCTTGGATGCAGTGAGGAGGAGGTACAGAGGTTTGTCCTAAGCTATCTGAATATGGTCTTCTTG TCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTTATAGAAGAAAAAATCAGTGCTTCACAAATAATTG AAAATCCGAGGATTTTAGACTCCAGCATAAATACTTTAAAAACTCGCATCCGAGAGCTGTCCCATGCTGG GTATGACTTGAGCACATCCAGCATTGCTCTGCTGTCCTGGAGTCAGAGAAGATATGAAGCTAAACTGAAG AGATTATGTGGATAGGACAGTGCAGGAGGAGTAGCAGGCAATCTGACAGGTGTGGCACTGGTTCCAGTTT GGACTTTTCAAGCAGGAGAGTTTTGTTTTTGTTTTTTAATTATTTTTTTTTAATTTTTTAAATAAAAATT TAAAAATATTTAAGATTAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001013023
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082778, AAH82778
• RefSeq Size: 1372 bp
• RefSeq ORF: 1140 bp
• Locus ID: 545725
• UniProt ID: Q8CHZ9
• Cytogenetics: 5 A1
• Gene Summary: Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.