Csf1r (NM_007779) Mouse Untagged Clone

CAT#: MC202732

Csf1r (untagged) - Mouse colony stimulating factor 1 receptor (Csf1r), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Csf1r
• Synonyms: Fms, CD115, Csfmr, Fim-2, CSF-1R, M-CSFR, AI323359
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066863 sequence for NM_007779
  CCAGATTCTGCCTCTTCCTCTGTTCCCTTTCAGGCAACCTAAAAAAAAAAAAAAAAAAAAAGGGGAAGAG GAGCCAGTGCAACAGACAGGAACGTGTTCATCTGTTCCCGTCCTCACAGAACTAGCAGCTGGGAGCCCCG TGCCCAGCCGACTCTCCAACCTGCATCGGCTCACGCTATCCCCTGGAGGCTATGGAGTTGGGGCCTCCTC TGGTCCTGCTGCTGGCCACAGTTTGGCATGGTCAGGGGGCCCCTGTCATCGAGCCTAGTGGCCCAGAACT GGTTGTAGAGCCGGGTGAAACGGTGACCCTGCGATGTGTGAGCAATGGCAGTGTGGAATGGGATGGCCCC ATCTCTCCCTACTGGACCTTGGACCCTGAATCTCCCGGAAGCACCCTGACCACAAGAAACGCGACCTTCA AAAACACTGGGACCTACCGTTGTACCGAGCTTGAAGACCCCATGGCAGGCAGTACCACCATCCACTTGTA TGTCAAAGATCCGGCCCACTCTTGGAATTTGCTGGCACAGGAGGTGACAGTGGTTGAGGGCCAGGAAGCT GTGCTGCCCTGTCTGATCACTGACCCTGCACTGAAGGACAGTGTCTCACTGATGCGTGAGGGGGGCAGGC AGGTCTTACGCAAAACGGTCTACTTCTTCTCGCCATGGCGAGGGTTCATTATCCGCAAGGCTAAAGTCCT TGACAGCAATACCTACGTGTGCAAGACCATGGTGAATGGTAGGGAATCCACCTCCACTGGCATCTGGCTT AAGGTGAATCGAGTCCACCCAGAGCCCCCACAGATAAAATTGGAGCCTAGCAAGCTGGTGCGGATTCGAG GGGAGGCTGCGCAGATCGTGTGCTCGGCCACTAACGCCGAAGTGGGATTCAACGTTATCCTCAAACGTGG AGACACCAAGCTGGAAATCCCCCTAAACAGTGACTTCCAAGATAACTATTATAAAAAAGTCCGGGCTCTC AGTCTCAACGCTGTGGACTTCCAAGACGCTGGCATATATTCTTGTGTGGCCAGCAATGATGTTGGCACAC GCACGGCCACCATGAACTTCCAGGTGGTGGAGAGTGCCTACTTAAACTTGACCTCTGAGCAGAGCCTCTT GCAGGAGGTGTCTGTGGGTGACAGCCTCATCCTCACGGTCCATGCAGATGCCTACCCTAGCATACAGCAT TACAACTGGACCTACCTAGGTCCATTCTTTGAAGACCAGCGCAAGCTTGAGTTTATCACCCAAAGGGCCA TATACAGGTACACATTCAAGCTCTTTCTGAACCGTGTAAAGGCCTCAGAGGCGGGCCAGTACTTCTTAAT GGCACAAAACAAGGCAGGCTGGAATAATCTGACCTTTGAGCTCACCCTGCGATATCCCCCAGAGGTCAGT GTTACATGGATGCCTGTGAATGGCTCTGATGTCCTGTTCTGTGACGTCTCTGGGTACCCTCAGCCCAGCG TGACATGGATGGAGTGCAGGGGCCACACCGATAGGTGTGATGAAGCCCAGGCTTTGCAGGTTTGGAATGA CACCCACCCTGAAGTCCTGAGTCAGAAGCCCTTCGACAAAGTGATCATTCAGAGCCAGCTGCCCATTGGG ACCTTAAAACACAACATGACTTATTTTTGCAAAACCCACAACAGTGTGGGTAACAGCTCTCAGTACTTCA GGGCCGTCTCCCTAGGACAAAGCAAGCAGCTCCCCGATGAGTCCCTCTTCACTCCGGTGGTGGTGGCCTG TATGTCTGTCATGTCTCTGCTGGTGCTACTGCTGTGGCTGCTCTTGTACAAGTACAAGCAGAAGCCGAAG TACCAGGTGCGCTGGAAGATCATCGAGAGATACGAAGGCAATAGCTACACCTTCATTGACCCTACTCAGT TGCCCTACAATGAGAAGTGGGAGTTCCCTCGGAACAACCTGCAGTTTGGTAAGACTCTAGGAGCCGGTGC CTTTGGGAAGGTGGTGGAGGCTACAGCCTTTGGTCTGGGCAAAGAAGATGCAGTGCTGAAGGTGGCTGTG AAGATGCTAAAGTCCACGGCTCATGCTGATGAGAAGGAGGCCCTGATGTCAGAGCTGAAGATCATGAGTC ACCTGGGACAGCACGAGAATATAGTCAACCTCTTGGGAGCCTGTACTCACGGAGGACCTGTCCTGGTCAT CACTGAATACTGCTGCTATGGAGACCTACTCAACTTTCTCCGAAGGAAGGCCGAGGCTATGCTAGGACCC AGCCTGAGTCCTGGTCAGGACTCCGAGGGAGACTCCAGCTACAAGAACATCCACCTGGAGAAGAAATATG TGCGCAGGGACAGTGGCTTCTCCAGTCAGGGTGTAGACACCTACGTGGAGATGAGGCCTGTCTCGACTTC TTCAAGTGACTCCTTCTTTAAGCAAGATCTGGACAAAGAGGCCAGCCGGCCCCTGGAGCTCTGGGACCTG CTCCACTTCTCCAGCCAAGTGGCTCAGGGCATGGCCTTCCTTGCTTCTAAAAACTGCATCCACCGGGACG TAGCAGCTCGAAACGTGCTGTTGACCAGCGGACATGTGGCCAAGATTGGGGACTTTGGACTGGCTAGGGA CATCATGAATGACTCCAACTATGTTGTCAAGGGCAATGCCCGCCTGCCTGTAAAGTGGATGGCCCCAGAG AGCATCTTTGACTGCGTCTACACAGTTCAGAGTGATGTGTGGTCCTACGGCATCCTCCTCTGGGAGATCT TCTCGCTTGGTCTGAACCCCTACCCCGGCATCCTAGTGAACAACAAGTTCTACAAACTGGTGAAGGATGG ATACCAAATGGCCCAGCCTGTATTTGCACCGAAGAACATATACAGCATCATGCAGTCCTGCTGGGACCTG GAGCCTACCAGAAGACCCACCTTCCAACAGATCTGCTTCCTCCTCCAGGAGCAGGCCCGACTGGAGAGGA GAGACCAGGACTATGCTAACCTGCCAAGCAGCGGTGGCAGCAGCGGCAGTGACAGTGGTGGTGGCAGCAG CGGTGGCAGCAGCAGTGAGCCAGAAGAGGAGAGCTCCAGTGAACACCTGGCCTGCTGTGAGCCAGGGGAC ATCGCCCAGCCCCTGCTGCAGCCTAACAACTACCAGTTCTGCTGAAGTGGGAGGGAGAGCCGAGTCCTGC CGCTCTCTACGTCCCAGCTTGGTCTCCTCCATGGCACGGGCGACATGGGGAGAACATATGGACTTCGCCC TCAGCTTGGCCCAGCTCTGACACTTCAGAACATGAGGGGTCTGGGGAGGTCAGAGGCCCCGTTTGTTCCC AGAGCCTGGGCCATCACTGCCAGTGGGGTTCTCACAGTGCTAGCCTCTATATTTACTATGCCAACTGGTG CACCCCTAGTTCTCTTTCTCCATCCTATTCCCATTTTAAAAAACCCGTCCCAAACTCTCGTGTTTCAATG GAAAGACTGATTTATGTCTCAAAAGACAAGAGTCTCAAAGGCTGTGGGTAAGCTGAAGGCTTGCCTCCCT GACAGATGCTTAGACTACAGGCTTCTTGGGACAGGTGGCCCCTTCCTAAGCTCACAGGAGTGGCCACCAC TCTTGACCTTCACTCTGTCTATAGTCCCGCCTCATCCTGGATCTTGTACTGAGCGGCAGCTAAAAGTGTT CTACCCAGTGCCCTGTCACTCTAGACTGGAAGGTATGGGGCCTGATGCAAGGCTGACCACACCAACAAAC ACCGTGTGCTCCTCTCCAAGCTGACTCGCCCTCATTAACTGTCAACATTAAACTAACAGCATTAACACAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007779
• Insert Size: 2934 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066863, AAH66863
• RefSeq Size: 3793 bp
• RefSeq ORF: 2934 bp
• Locus ID: 12978
• Cytogenetics: 18 34.41 cM
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.[UniProtKB/Swiss-Prot Function]