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Gak (NM_153569) Mouse Untagged Clone

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SKU
MC202684
Gak (untagged) - Mouse cyclin G associated kinase (Gak), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,137.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gak
Synonyms D130045N16Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC060622 sequence for NM_153569
GATGGCCGGGTTGTTCGCCCGCAGCTGCTAACCCGGCAGTGCGGAGCTGGAGCCTGGCTCCCGCTGCCGC GGAGCGGCCGACGCCATGTCGCTGCTGCAGTCTGCGCTGGACTTCCTGGCGGGCCCTGGTTCTCTTGGCG GAGCTGCGGGCCGTGATCAGAGTGACTTCGTGGGGCAGACAGTGGAGCTGGGCGAGCTGCGTCTGCGGGT GCGGCGGGTCCTGGCCGAGGGAGGGTTTGCTTTTGTTTATGAAGCTCAAGATCTGGGAAGTGGCAGAGAG TATGCATTAAAGCGATTACTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAGGAAGTTTGTTTCT TGAAAAAACTTTCTGGCCACCCCAATATTGTCCAGTTCTGCTCTGCAGCATCCATAGGAAAAGAGGAGTC GGACACTGGGCAGGCTGAGTTCCTCCTGCTTACGGAGCTTTGTAAAGGACAGCTGGTGGAGTTTCTCAAG AGAGTTGAATGTAAAGGTCCTCTGTCCTGCGACAGCATTCTGAAGATCTTCTACCAGACATGCAGAGCAG TGCAGCACATGCACAGGCAGAAACCACCCATCATCCACAGAGATCTCAAGGTTGAAAACTTACTGCTTAG TAACCAGGGGACCATTAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGC TGGAGTGCCCAGAAGCGAGCCATGGTGGAGGAAGAGATCACAAGGAACACCACACCCATGTACAGAACAC CAGAAATTGTAGACCTGTATTCCAACTTCCCCATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTAT CTTATACCTGCTGTGTTTCCGGCAGCATCCTTTTGAGGATGGAGCAAAACTTCGGATAGTCAATGGGAAG TATTCCATTCCTGTGAATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGCCATGCTAAAGGTCA ACCCAGTGGAGAGGCTGTCCATTGCTGAAGTGGTCCGACAACTGCAGGAAATTGCAGCAGCTAGGAATGT GAACCCCAAAGCTCCCATCACAGAGCTGCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGA GCACAACCACCTTGCGGGGGCACCGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCT ACGGTGGGTTTCTTGATATTCTGCGGGGTGGGACCGAACGGCTCTTCACCAACCTCAAGGATACTTCCTC CAAGGTCATCCAGTCTGTGGCTAACTATGCAAAGGGTGATCTGGACATATCTTACATCACATCCAGGATT GCAGTGATGTCATTCCCAGCAGAAGGTGTGGAGTCTGCAATCAAAAACAATATAGAGGATGTGCGAATGT TTCTGGATGCTAAGCATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGGATATACCGGGCTTCCAA GTTTCACAACCGGGTCACTGAGTGTGGCTGGGCAGTCAGACGGGCACCACATCTCCACAGTTTGTATACT CTATGTAGGAGCATGCATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTGCATTGCATGGATG GGAGAGCTGCATCTGCTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGC TGCTGTGTACATGTTCAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAA TATGTATGTGACATGGTGGCAGAGGAGCCCATCACGCCCCATAGCAAGCCAATGCTGGTGAAATCTGTTG TCATGACCCCCGTGCCACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCGTTCTGTGAGGTCTACGTTGG AGAGGAGCGTGTCACCACCACGTCGCAGGAATATGACAGAATGAAGGAATTTAAAATTGAGGATGGCAAG GCTGTCATCCCCCTGGGCGTAACAGTTCAAGGAGACGTGCTCATCATCATCTACCATGCCAGGGCCACAC TGGGAGGGAGGCTGCAGGCTAAGATGGCGTCCATGAAAATGTTCCAGATCCAGTTCCACACTGGGTTCGT GCCTCGAAACGCAACCACTGTGAAATTTGCAAAATATGACCTGGATGCTTGTGATATTCAAGAGAAGTAC CCAGATCTGTTCCAGGTGAACCTGGAAGTGGAGGTAGAGCCTAGAGACAGGCCCAGCCGAGAAGCTCCAC CTTGGGAGAACACCAGCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAGCAGGA CATTCTGTCTAAGTTTGGGAAGCCGGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAG GCAGGGTCTCCGGAGGCTGAGATCACAGAGTCGGACTCACCGCAGAGCAGCAGTACGGACACCAACCACT TTCTTCACACACTGGATTGGCAGGAGGAAAAAGAACCAGAGACTGGGTTAGACAATACCTCTCCTAAGGA GAGTCAGTCTGTCCTGATTGCAGACGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATTCCCC AGTGAGGAGAGGAAACCAGGAGCTGGAGAAGATACACCAAGGCTGGCTGCTGGGACCAAACAGCAAGACT TAATATTTGATGTGGGCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCCGAAGAAGGTGTTGATCTCCT GGGGCTACACTCTGAGGGGGACTTAAGGCCTGCTGCCCCCTTACAGGCTTGTGGGGTCCCATCTAGCAAC ACTGACCTGTTGAGCTGCCTTCTTGAACCATCTGATGCTGCTCAAGTAGGGCCTCCTGGTGACCTGCTTG GTGGTGAGGCTCCACTGCTGCTAGCAAGCCCAGTTTCTCCTCTTGGGCTGCAGAACAACCTACAAGGAAA AGTCCCTGACACTGTGGACCCATTTGACCAGTTCCTGCTGTCATCCAACTCAGACACCCAGCCCTGCTCC AAGCCTGATCTCTTTGGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCCACCC ACAGTGCCCCACCCCCATCCTGTAGCACTGCCTTCCTGCACCTAGGGGATCTGCCAGCAGAGCCCAGCAA GGTAATAGCTTCATCCAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGACACAGCTACACCT GGGCCAGCCTCCATTCCAGTACCAGAAGGTACCCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCTGGCC CCAACCCCAGCCAAACCAAGTCTCAGAACCTTGACCCATTTGCTGATCTCAGTGATCTCAGCTCTAGCCT CCAAGGCCTGCCTGCTGGACTTCCTGCAGGGGGCTTTGTTGGCGCACCAGCCCCCACTCAAAAGAGCAAC AGCCCCTGGCAGGCAAATCGTCCCACAGCCCCTGGAACCTCATGGACCCCCCAGGCCAAGCCAGCCCCCA GAGCCTCTGAACAGCTAAGGTCTCACTTCAGTGTGATCGGGGCCCGAGAAGAGAGGGGCGTCCGTGTGCC CAGCTTTGCCCAAAAGCCAAAGGTCTCAGAAAATGATTTTGAAGATCTTCTGCCTAATCAAGGCTTCTCT AAGTCTGACAAGAAGGGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACC CACTCAAATTGAAGCTTTTGGACTGGATTGAAGGCAAGGAGAGGAACATTCGTGCACTGCTGTCCACTCT GCACACGGTATTGTGGGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAG CAGGTGAAGAAGCAGTACCGCCGTGCAGTGCTGGTAGTTCATCCTGATAAGGCCACAGGGCAGCCGTATG AACAGTATGCCAAGATGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCGAG GCCCCTCTTCTGAGGCCTGTGATGGCTATGGCTGCAGGAGCTGTTCATGAATCCTGAATGCTGCTGGCCT GACTGAGGCACAAATGGGACCATAACAGGTGTGGCAGGTGATATCGCCCCAGGGGCCTGGACCATCTACT CCCAATCATCCCTGCATTCATCATGGACAAATACCATTCCATGGCTGGGAGAGAAAGCATTCCTCAGATC TGATGTTAGTTGTTTTGTTTTTCTTTTTCTTGTACCAGAGGAAAAGTGTTGATTTATATTCCTTCCACAG CTGTCACAGTTTGTGATTTACTTGGTGTCTGGTGGCTGTCACCTCTTGGATGGCACATGTCGCTGGCACT TCTCTCCTGTCATTTCTTCAAATATGGGGTACATCCACATCCTACTCTGATCAGTTTGCCAATCATCTGT ACATAATTAAACTATTTTCTGATGGCTGGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Restriction Sites AscI-NotI |
ACCN NM_153569
Insert Size 3918 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC060622, AAH60622
RefSeq Size 4486 bp
RefSeq ORF 3918 bp
Locus ID 231580
UniProt ID Q99KY4
Cytogenetics 5 F
Summary Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_153569" in other vectors (4)
SKU Description Size Price
MG211904 Gak (tGFP-tagged) - Mouse cyclin G associated kinase (Gak) 10 ug
$1,336.00
MR211904 Gak (Myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak) 10 ug
$589.00 $1,136.00
MR211904L3 Lenti ORF clone of Gak (Myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak) 10 ug
$1,436.00
MR211904L4 Lenti ORF clone of Gak (mGFP-tagged) - Mouse cyclin G associated kinase (Gak) 10 ug
$1,436.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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