Nfib (NM_008687) Mouse Untagged Clone

CAT#: MC202677

Nfib (untagged) - Mouse nuclear factor I/B (Nfib), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfib
• Synonyms: 6720429L07Rik; CTF; E030026I10Rik; NF-I/B; NF1-B; NFI-B
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC062908 sequence for NM_008687
  AACAACTGCAAAACAGAAAACAAATCCCCTAAACCAGGCGACCAGCAGCCTGCACCAGCGGCGGCAGCCT CGGAGAGCTCGGCCGGCGCGCGCGAACCGCAAGAGGGTTAAAAGTGTAGATTGGATTTCACCCCGGGAAA TCTAGCTCGCCGAGTGAACTTGAATCTTTGGCTATTTAAGGAGGACTGGGTTTGTTGCGAAGTTGCGGTG AACCGGCGCAGTGCCCCGTCCTGATTGATCGCATCGCTGGGCTCAGATGACTGTAAAATGAATAGATGAA ATCCTTGCTTCTGGAAGATTTTCTTGGGCATCTCCGGGAAAGTGCGTTTTAAGGCGAAGTCATGATGTAT TCTCCCATCTGTCTCACTCAGGATGAATTCCACCCATTTATTGAGGCACTTCTTCCTCACGTCCGTGCAA TTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAAACGAATGTC GAAGGATGAAGAAAGGGCAGTCAAAGACGAGCTGCTCAGTGAGAAGCCCGAAATCAAGCAGAAGTGGGCA TCCAGGCTCCTGGCCAAACTGCGCAAAGATATCCGCCAGGAGTACCGGGAGGACTTTGTGCTTACCGTGA CTGGCAAGAAGCACCCGTGCTGTGTCTTATCCAATCCAGACCAGAAGGGTAAGATTAGGAGGATCGACTG CCTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGCATCCCTTTG GAGAGTACGGATGGAGAGCGACTCATGAAGTCCCCGCACTGCACAAACCCAGCACTTTGTGTTCAGCCAC ACCACATCACAGTATCAGTTAAGGAGCTTGACTTGTTTTTGGCATACTACGTGCAGGAGCAAGATTCTGG ACAATCAGGAAGTCCAAGCCACAGTGATCCTGCCAAGAATCCTCCAGGGTACCTCGAGGACAGCTTTGTA AAATCCGGAGTCTTCAATGTATCAGAGCTTGTGAGAGTATCCAGAACACCCATAACCCAGGGAACTGGAG TCAACTTCCCAATCGGAGAAATTCCCAGCCAACCATACTATCATGACATGAACTCTGGTGTGAACCTGCA GAGGTCGCTGTCTTCTCCACCGAGCAGCAAAAGACCCAAAACTATATCTATAGATGAAAATATGGAGCCA AGTCCTACAGGAGACTTTTACCCCTCTCCAAATTCACCAGCTGCTGGAAGTCGAACATGGCATGAACGAG ATCAAGATATGTCTTCTCCAACTACAATGAAGAAGCCTGAGAAGCCACTGTTTAGCTCTACATCTCCACA GGATTCTTCCCCAAGATTGAGCACTTTCCCCCAGCACCATCATCCCGGAATACCTGGAGTCGCGCACAGT GTCATCTCAACTCGAACTCCACCTCCGCCCTCACCGTTGCCATTTCCGACGCAAGCTATCCTTCCTCCGG CACCTTCCAGCTACTTCTCTCATCCAACAATCAGATATCCTCCTCACCTGAATCCTCAGGATACTCTGAA GAACTACGTACCTTCTTATGACCCATCCAGTCCTCAAACGAGCCAGTCCTGGTACCTGGGCTAGCTTGGT TCCTTTTCAAGTGTCAAATAGGACACCCATCTTACCGGCAAATGTCCAAAATTATGGTTTGAACATAATT GGAGAGCCTTTCCTTCAAGCGGAGACAAGCAACTGAGGGGAAAGAACCACAACCATAGTATAAGAAAATT AGGGGAAAAACACCCAACAAAGGAAAAGAGGAAGACTGGACAACAGGGAGAAAGGAGAGAAACTGAAGAA AGAAGACGGTAGACCAGCATTGCAGCACTTACAGTCACTAATTCCCTTAAGGTTGAGACTATAGTGACAG GAAAGGGTCAATGACATCTCACTGATGCTAGACTGCAGCCCCTGCACCGTAGCCTTTGTTACATGAAGTC CGCTGGGAAATAGATGTTCTGTCTCTATGACAATATATTTTAACTGACTTTCTAGATGCCTTAATATTTG CATGATAAGCTAGTTTTCTTGGTTAGTATTCTTGTTGTTTACGCATGGAGTCACTATTCCTGGTTATCTC AACAACGAAGGCTAGGAGGCGGCGTCAGAGGTGCTGGGGACAGAGCCATGAGCCAGCCATTTTATAAGCA CTCTGATTTCTAAAAGTTAAAATTATATATATATATATATATATATATAAAAGAATCTCTGTAGCCTTTA GTTATCCGTACAGATTTATTAAATTTTGGCCCTTAACCCAGCCTTTTCCAGTGTGTAACCCAGTTTGAAA TCTTAAAAAGAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_008687
• Insert Size: 1263 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062908, AAH62908
• RefSeq Size: 2338 bp
• RefSeq ORF: 1263 bp
• Locus ID: 18028
• UniProt ID: P97863
• Cytogenetics: 4 38.4 cM
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks three alternate exons in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.