Med15 (NM_001040683) Mouse Untagged Clone

CAT#: MC202625

Med15 (untagged) - Mouse mediator complex subunit 15 (Med15), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med15
• Synonyms: A230074L19Rik; AW536074; mPcqap; Pcqap
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC054779 sequence for NM_001040683
  TGTCGCTGTGGTAGAGGCGGCGGCGCCGGGGGTGGCCGGTAGGGACTCGGGCTACAAGAGTAGGGGCTCC GGGCATGGACGTTTCGGGGCAGGAGACCGACTGGCGTAGCGCCGCCTTTCGGCAGAAGCTGGTCAGCCAA ATTGAGGATGCCATGAGGAAAGCTGGTGTGGCCCACAGTAAATCTAGCAAGGATATGGAGAGTCATGTGT TCCTGAAGGCCAAGACCCGGGATGAGTATCTTTCCCTTGTGGCCCGACTCATTATCCATTTCCGAGATAT TCATAACAAGAAATCCCAAGCTTCTGTCAGTGACCCCATGAATGCACTGCAGAGCCTTACTGGTGGACCC ACCCCAGGAGCAGCTGGGATTGGCATGCCTCCTCGGGGCCCAGGACAGTCCCTGGGTGGGATGGGTGGCC TTGGCGCTATGGGACAACCACTGCCCCTCTCCGGGCAACCACCCCCTGGAACCTCTGGAATGGCCCCTCA TGGCATGGCTGTGGTGTCTACAGCAACTCCACAGACTCAGCTGCAGCTCCAGCAAGTGGCATTGCAGCAA CAGCAGCAGCAGCAACAACAACAGCAATTCCAGCAACAGCAGGCAGCACTGCAGCAACATCAGCAACAGC AGCAGCAGCAACAGCAGCAACAGCAGTTCCAGGCACAACAGAATGCCATGCAGCAACAGTTCCAAGCAGT AGTGCAGCAGCAGCAGCTTCAGCAGCAGCAGCAGCAGCAGCACCTGATTAAGTTGCATCATCAAAGCCAG CAACAACAGATACAACAGCAGCAACTGCAGAGGATGGCACAGTTGCAGCTGCAGCAACAGCAACAGCAGC AACAGCAGCAGGCTTTGCAGGCCCAGCCACCAATGCAGCAGCCATCAATGCAGCAGCCACAGCCTCCCCC TTCTCAGGCCCTACCCCAGCAGCTGTCACAGCTGCATCATCCACAGCATCACCAGCCACCACCTCAGGCT CAGCAGTCCCCCATTGCTCAAAACCAACCACCACAGATCCCATCACAGTCACAGAGCCAGCCTTTGGTGT CACAAGCACAAGCCCTTCCTGGACCGATGCTGTATGCTGCCCAACAGCAGCTGAAATTTGTCCGTGCTCC GATGGTGGTCCAGCAGCCGCAAGTGCAGCCCCAGGTGCAGCAGGTGCAGCCCCAGGTGCAGCCGCAGGCA GCAGTGCAGGCAGCACAGTCTGCCCAGATGGTAGCTCCCGGCGTCCAGGTCAGCCAAAGCAGCCTCACCA TGCTGTCCTCACCGTCACCAGGCCAGCAGGTGCAGACCCCACAGTCGATGCCACCTCCCCCACAGCCGTC CCCACAACCTGGCTCACAGCCCAACTCTAATGTCAGCTCCGGCCCTGCCCCATCTCCCAGCAGCTTCCTG CCTAGCCCTTCACCACAGCCTTCTCAGAGCCCAGTGACAGCACGCACCCCACAGAACTTCAGCGTTCCTT CCCCTGGACCTTTAAACACCCCTGTGAACCCCAGCTCTGTCATGAGCCCAGCTGGCTCTAGCCAGGCTGA GGAGCAGCAGTACCTGGACAAGCTGAAGCAATTGTCCAAGTACATCGAGCCCCTGCGACGCATGATCAAC AAGATCGACAAGAATGAAGACAGAAAAAAGGACTTAAGTAAGATGAAGAGCCTGCTGGACATCCTCACCG ACCCCTCGAAGAGGTGTCCCCTGAAGACCCTGCAAAAGTGTGAGATTGCGCTGGAGAAGCTCAAGAATGA CATGGCAGTGCCCACACCCCCACCACCCCCAGTTCTTCCAACCAAACAGCAGGACCTGTGCCAACCACTC CTAGATGCAGTCCTGGCCAACATCCGTTCACCTGTCTTCAACCATTCCCTGTACCGCACATTTGTGCCAG CCATGATGGCCATCCATGGCCCACCTATCGTGTCGCCAGTGGTGTGTTCCCGGAAGCGCCGGTTTGAGGA GGATGAGCGGCAGAGCATACCCAATGTGCTGCAAGGTGAAGTGGCAAGGCTGGATCCCAAGTTCCTGGTG AACTTGGACCCTTCTCACTGCAGCAACAACGGTACTGTCCACCTGATCTGCAAGCTGGATGATAAGGACC TCCCTAGTGTGCCACCACTGGAGCTCAGTGTGCCTGCTGACTACCCTGCCCAGAGCCCAATGTGGATCGA CCGTCAGTGGCAATATGATGCCAACCCCTTTCTGCAGTCAGTGCACCGGTGCATGACCTCAAGGCTGCTG CAGCTCCCTGACAAGCACTCGGTCACAGCGCTGCTCAACACCTGGGCCCAGAGCATCCACCAGGCCTGCC TCTCAGCTGCCTAGCAAACTGGAACTTCAGGACTGCCAGCAGCCCTTCTGGCTAAAGATCTCACGCCGCC TACCAGACATTACTAAGTGTTGGCTTCTTAGAGGGCCTGGGTTAGGTTAGCTTTCCTGCTTTTACCTTCT TTGGTGGGGACCTGCCAGCTGTTTCCCATTCTTGTGCAGTATTGACTGGGCAGCTGTGAAGCTGGCTGCT TGAGGGCCTGGCGGTTTCCCCAGAAGGTTTTCCATTGGTTTGGTTTGTTTCGTTATTTTTTGTTTTTTGT TTTTGCAGAGGTCAGGCCCCACACTTCCTCCAGCCTTTCTGGGCTTCCATGCCCACTCAGCAGCATGAGA ACTCAGGGCCCCATCAAAGCATCTCTGTTTTGCAATACATTGTACTCATTATGTGGAGAATGTGCAGGAC ATAGGAAACCCTCACTCTATACACAGTTCTCCAGTCACACTGGGGCTCAGGCGTGCCTCCTAAGGCATGT GGACACGTCACAGGAACACGTCTGAATCCAGGGCACACATTTCAGTTTGGAAGAAAGACCAATAGGGCAT CCGCAGGATCTCTGCCACCAGCAGAACCTCCACTTTCTGGGCCTCCTCAGTCATGTGAGGAGGAGAATCA GCTGGAGGCAAGGGGCTGTCCCTGACATTCCTGCGCCAATCTTGGGTGAAGGGCTGTCAGTGCTATGATA CACAGGCAGATAGGCAGATACTATCACTGGGACCCACCAGCCTCAACACCATATTCTCTTATTCTCTATA CCACCACCAAGCGTGTGGGTTTCCTTCGGAATACTGCAGATGGCGCTCGCCACACTGGGCAGCAATGGGG AGCACTCTGGTGAAAATTCCCCTTTTTATATGTGCACTACTCTGTCATATGATTCTTATAATAAATTTAT TATTCCTGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_001040683
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054779, AAH54779
• RefSeq Size: 3248 bp
• RefSeq ORF: 2250 bp
• Locus ID: 94112
• Cytogenetics: 16 10.94 cM
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.