Vcp (NM_009503) Mouse Untagged Clone

CAT#: MC202620

Vcp (untagged) - Mouse valosin containing protein (Vcp), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vcp
• Synonyms: 3110001E05; CDC48; p97; p97/VCP
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049114 sequence for NM_009503
  TGATTCGGCTTTTCTCGGTTCAGTCTCCGTGAAGCGTTTGCAGCCGTCGTTTGATTAGTCGCTGCCCCCC TCGCGGATTAGGAGCTAGCGTCTCCCGCCCGCCCGCCTGCCGCCCCGGTGCCCCTGGGAGGAAGCGAGAG GGAGGCTGCCAGAGGGTTTGTCACTGCTGTTGCTCCTCCGCCTCAGCGAGTCCAGCCCCGGCCTAGTCGG TCGCCTGCCTTTCTCATAGCCGTTACCCTCAGGCCGCCACAGCCGCCGACCGGGAGAGGCGCGCGCCATG GCCTCTGGAGCCGATTCAAAAGGTGATGATTTATCAACAGCCATTCTCAAACAGAAGAACCGACCCAATC GGTTAATTGTTGATGAAGCCATCAATGAAGATAACAGCGTGGTGTCCTTGTCCCAGCCCAAGATGGATGA ACTGCAGTTGTTCCGAGGTGACACGGTGTTGCTAAAAGGAAAGAAAAGACGGGAAGCTGTATGCATTGTT CTTTCTGATGACACGTGTTCTGATGAGAAGATTCGAATGAATAGAGTTGTTCGGAATAACCTCCGAGTTC GCCTAGGAGATGTCATCAGCATCCAGCCATGCCCTGATGTAAAGTATGGCAAACGTATCCACGTTCTACC CATCGATGACACAGTGGAAGGCATCACTGGCAATCTCTTTGAGGTATACCTTAAGCCGTACTTCCTGGAA GCTTATCGGCCCATCCGTAAAGGAGATATTTTTCTTGTCCGGGGTGGGATGCGTGCTGTGGAGTTCAAAG TTGTAGAGACAGATCCCAGCCCTTACTGTATTGTTGCTCCAGACACAGTGATCCACTGTGAGGGGGAGCC AATCAAGCGAGAGGATGAGGAGGAATCCTTGAATGAAGTAGGCTATGATGACATCGGTGGTTGCAGGAAG CAGCTAGCTCAGATAAAGGAGATGGTGGAGCTGCCACTGAGACATCCTGCGCTCTTTAAGGCGATTGGTG TAAAGCCTCCTCGGGGAATCTTGTTGTATGGGCCTCCTGGGACAGGGAAGACCCTGATTGCTCGAGCTGT GGCAAATGAAACTGGAGCCTTCTTCTTTCTGATCAATGGTCCTGAAATCATGAGCAAATTGGCTGGTGAG TCTGAGAGCAACCTTCGTAAAGCCTTTGAGGAAGCTGAAAAGAATGCTCCTGCTATCATCTTCATCGATG AGCTTGATGCCATTGCACCCAAAAGAGAGAAAACTCATGGGGAAGTGGAGCGTCGCATCGTGTCTCAGTT GTTGACCCTCATGGATGGCCTAAAGCAGAGAGCACATGTGATAGTTATGGCAGCAACCAATAGACCCAAC AGCATTGACCCAGCCCTACGGCGATTTGGTCGCTTTGACAGAGAGGTAGATATTGGAATACCTGATGCTA CAGGACGTTTGGAGATTCTTCAGATCCATACCAAGAACATGAAACTGGCAGATGATGTGGACTTGGAACA GGTAGCCAATGAGACTCATGGTCATGTTGGTGCTGATTTGGCAGCCCTATGTTCAGAGGCTGCTCTGCAG GCCATCCGGAAAAAAATGGACCTCATTGACCTAGAAGATGAGACCATTGATGCTGAGGTCATGAATTCCC TGGCAGTTACTATGGATGACTTCCGGTGGGCTTTGAGTCAAAGCAACCCATCAGCACTTCGGGAAACTGT GGTAGAGGTGCCACAAGTAACCTGGGAAGATATTGGAGGCCTGGAGGATGTCAAACGTGAGCTTCAGGAG TTGGTTCAGTATCCTGTGGAACATCCAGACAAATTCCTCAAATTTGGCATGACTCCCTCCAAAGGCGTTC TTTTCTATGGACCTCCTGGCTGTGGGAAAACCTTACTGGCTAAAGCCATTGCTAATGAATGCCAGGCCAA CTTCATCTCCATCAAGGGTCCTGAGCTGCTTACCATGTGGTTTGGGGAATCTGAGGCCAATGTCCGGGAA ATTTTTGACAAGGCACGGCAAGCTGCCCCCTGTGTACTCTTCTTTGATGAGTTAGATTCAATTGCCAAGG CTCGAGGTGGTAATATTGGAGATGGTGGTGGAGCTGCTGACCGAGTCATCAATCAGATCCTGACAGAAAT GGATGGCATGTCTACAAAAAAGAATGTGTTTATCATTGGAGCTACCAACAGGCCTGACATCATTGATCCT GCTATCCTAAGACCTGGCCGTCTAGATCAGCTCATTTATATCCCACTTCCTGATGAGAAGTCCCGTGTTG CCATCCTAAAAGCCAATCTGCGAAAGTCCCCAGTTGCCAAGGATGTGGATTTGGAGTTCCTGGCTAAGAT GACTAATGGCTTTTCTGGAGCTGATTTGACAGAAATTTGCCAACGGGCTTGTAAACTGGCCATTCGTGAA TCTATTGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAATCCATCGGCTATGGAGGTAGAAGAGG ACGATCCAGTGCCTGAGATCCGCAGAGATCACTTTGAGGAAGCCATGCGTTTTGCCCGACGTTCTGTCAG CGATAATGACATTCGGAAGTATGAAATGTTCGCCCAGACACTGCAGCAGAGTCGAGGTTTTGGCAGCTTC AGATTCCCTTCAGGGAACCAGGGTGGAGCTGGTCCCAGCCAGGGCAGTGGAGGTGGCACAGGTGGCAGTG TGTACACAGAAGACAATGACGATGACCTGTATGGCTAAGTGATGTGCCAGCATGCAGCGAGCTGGCCTGG CTGGACCTTGTTCCCTGGGGGAGGGGGCGCTTGCCCAAGAGGGACCAGGGGTGTGCCCATGGCCTGTTCC ATTCCTCAGTCTGAACAGTTCAGCCCCAGTCAGACTCTGGACAGGGGTTTCCTGTTGCAAAAAAAAAAAA AAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_009503
• Insert Size: 2421 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049114, AAH49114
• RefSeq Size: 2876 bp
• RefSeq ORF: 2421 bp
• Locus ID: 269523
• UniProt ID: Q01853
• Cytogenetics: 4 22.95 cM
• Gene Summary: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Plays a role in the regulation of stress granules (SGs) clearance process upon arsenite-induced response (By similarity). Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with DDX58/RIG-I: interaction takes place when DDX58/RIG-I is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58/RIG-I. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.[UniProtKB/Swiss-Prot Function]