Snx1 (NM_019727) Mouse Untagged Clone

CAT#: MC202614

Snx1 (untagged) - Mouse sorting nexin 1 (Snx1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Snx1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066044 sequence for NM_019727
  CCTCACACGGCTGGAGCGCTTTGCTCGCGGCACTGTCTATAAAGTTGTTGTTGCTGCTTCCGCCACGGGT GGAGGAAGATGGCGTCAGGAGGTGGTGGCTGTAGCGCTTCGGAGCGACTCCCTCCGCCCTTCCCTGGCAT GGATCCGGAGTCGGAAGGGGCTGCCGGGGGGTCAGAACCCGAGGCTGGGGACAGCGACACCGAGGGGGAG GATATTTTCACTGGCGCCGCGGCAGCCACTAAGCCCCAGTCTCCAAAGAAAACCACATCCCTTTTTCCTA TCAAAAATGGTTCCAAAGAGAATGGGATCCATGAGGACCAAGACCAAGAGCCACAGGATCTCTTTGCAGA TGCCACAGTGGAACTATCCCTGGACAGCACACAGAATAACCAGAAGACAATGCCAGGCAAAACCCTTACT TCTCATCCTCCGCAAGAAGCCACAAATTCTCCGAAGCCACAGCCAAGCTATGAGGAGCTGGAGGAAGAAC AGGAGGATCAGTTTGATTTGACAGTTGGTATCACCGATCCTGAGAAGATCGGGGATGGTATGAATGCCTA TGTAGCCTACAAAGTCACCACACAGACAAGCTTACCGATGTTCAGAAGTAGGCAGTTTGCAGTGAAAAGA AGATTCAGTGACTTCCTGGGTCTTTATGAGAAGCTTTCAGAGAAGCACTCCCAGAATGGTTTCATTGTGC CTCCTCCACCCGAGAAGAGCCTAATAGGAATGACAAAGGTGAAGGTTGGGAAGGAAGATTCTTCTTCCGC AGAGTTTCTTGAGAAACGGAGGGCTGCTTTGGAAAGGTACCTTCAAAGAATTGTGAATCATCCCACCATG TTACAGGACCCAGATGTCAGGGAGTTTCTGGAAAAAGAAGAGCTGCCTCGAGCTGTGGGCACGCAGGCTT TGAGTGGCGCTGGTCTCCTTAAGATGTTCAACAAAGCTACAGATGCAGTCAGCAAAATGACCATCAAGAT GAATGAATCAGACATTTGGTTCGAGGAGAAACTCCAGGAGGTAGAGTGTGAGGAGCAGCGCCTACGGAAG CTGCATGCTGTGGTGGAGACTCTAGTCAACCATAGGAAAGAACTAGCGCTGAACACAGCCCTGTTTGCCA AGAGTCTAGCCATGCTTGGGAGCTCCGAGGACAACACAGCACTTTCTCGAGCGCTCTCCCAACTGGCTGA GGTGGAAGAAAAGATTGAGCAGCTCCACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAACTC CTGAGTGACTATATTCGCCTCCTGGCCATTGTCCGAGCTGCCTTCGACCAGCGCATGAAGACATGGCAGC GCTGGCAGGATGCTCAAGCCACGCTGCAGAAGAAGCGGGAGTCTGAGGCACGGCTGCTGTGGGCCAACAA GCCTGACAAACTGCAGCAGGCTAAGGATGAGATCACAGAGTGGGAATCTCGGGTGACTCAGTATGAAAGG GACTTTGAAAGGATTTCCACAGTTGTCCGAAAGGAAGTGACACGATTTGAGAAAGAGAAGTCCAAGGATT TCAAAAACCACGTGATGAAGTACCTGGAGACACTCCTACATTCCCAGCAGCAGCTGGCAAAATACTGGGA AGCCTTCCTTCCTGAGGCAAAGGCCATCTCCTGAGGGACCAAGGACACCAGAGCCTACCTTCGTGACGCT GCCTTTTTATACACTGTCCTTCTTCCTCGATGGACCCTAACTGATGCCCCTGCCTTGGCTGGACTTAGCC CTTCCACTCTGCCCCACAGACAAACTGTTCCCAGTTACTCTAACCAACATTTCATTTAGCTTCCACGTAT ATTTTCTTACCTAAGAGAATAGTTTCCTGCTTTAAGCAAAAGACCTACAATAGGTGGTGGAATTATAGGT TGGGGTGGAGTGTTGATATAAATATATAAATACAAATGTATATTTTTCAGGATGTGGTTTAGGAACTGGG AATAACGTTTTCTGTTACTCCTGATGGTGCCATGAAAGATTATGTAATAAAATATTTGAAAATCATGAAA AAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_019727
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066044, AAH66044
• RefSeq Size: 2046 bp
• RefSeq ORF: 1566 bp
• Locus ID: 56440
• UniProt ID: Q9WV80
• Cytogenetics: 9 C
• Gene Summary: Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1). Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking (By similarity).[UniProtKB/Swiss-Prot Function]