Ywhaz (NM_011740) Mouse Untagged Clone

CAT#: MC202613

Ywhaz (untagged) - Mouse tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (Ywhaz), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ywhaz
• Synonyms: 14-3-3zeta; 1110013I11Rik; AI596267; AL022924; AU020854
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050891 sequence for NM_011740
  GGTGTGGACGCCGAGCTCGCGACTGGAAAGGCAGGGCGTCATTCAGCCTCGCGCTTTTCCCAGCCTTAAA AGGTCTAAGGCCGCTTCCTGCAGCCAGAAGCGTTTGACGTCATCGTGCGTGTGGTGCCCCTGCTGCCGGG GCTAGTGATTGGAGGAAACCCCGTGTCTGCGGAGCGGCTGTAGCCTGTGAGCAGCCAGATCAGGGACAGA GTCTCAGCCTCGCCGCCACCGCTGCTGCCGCCGCCGCCCAGAGACCGCCGAGCCGCGTCCGTGTGCCGCC ACCCACTCCGGACACAGAATATCCAGTTATGGATAAAAATGAGCTGGTGCAGAAGGCCAAGCTGGCCGAG CAGGCAGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTCACTGAGCAGGGAGCTGAGCTGTCGA ATGAGGAGAGAAACCTTCTCTCTGTTGCTTATAAAAACGTTGTAGGAGCCCGTAGGTCATCGTGGAGGGT CGTCTCAAGTATTGAGCAGAAGACGGAAGGTGCTGAGAAAAAGCAGCAGATGGCTCGAGAATACAGAGAG AAGATCGAGACGGAGCTGCGTGACATCTGCAACGATGTACTGTCTCTTTTGGAAAAGTTCTTGATCCCCA ATGCTTCGCAACCAGAAAGCAAAGTCTTCTATTTGAAAATGAAGGGTGACTACTACCGTTACTTGGCCGA GGTTGCTGCTGGTGATGACAAGAAAGGAATTGTGGACCAGTCACAGCAAGCATACCAAGAAGCATTTGAA ATCAGCAAAAAGGAGATGCAGCCGACACACCCCATCAGACTGGGTCTGGCCCTCAACTTCTCTGTGTTCT ATTACGAGATCCTGAACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAAACAGCTTTCGATGAAGCCATTGC TGAACTTGATACATTAAGTGAAGAGTCGTACAAAGACAGCACGCTAATAATGCAGTTACTGAGAGACAAC TTAACATTGTGGACATCGGATACCCAAGGAGATGAAGCAGAAGCAGGAGAAGGAGGGGAAAATTAACCGG CCTTCCAACCTTTGTCTGCCTCATTCTAAAATTTACACAGTAGACCATTTGTCATCCATGCTGTCCCACA GATAGTTTTTTTGTTTACGATTTATGACAGGTTTATGTTACTTCTATTTGAATTTCTATATTTCCCATGT GGTTTTTATGTTTTAATATTAGGGGAGTAGAGCCAGTTAACTTTAGGGAGTTACTCATTTTCATCTTGAG GTGGCCAATATGGGATGTGGAATTTTTACATGAGTTACACATGTTTGGCATAGTACTTTTGGTACATTGT GGCTTCAGAAGGGCCAGTGTTAAAACTGCTTCCATGTCTAAGCAAAGAAAACTGCCTACATATTGGTGTG TGCTGGCGGGGAATAATCAGGATAATGGGTTCCAGTCATGAGTGTAGTCTTTGTGGGTACTGTAAGGCCT GGAGCACTTGTGAGGCTGTGACACAAACACCCTGTGGATGCACGCTAGGATCGTGTGTGTCTGCGTGCAC ACTCTTGACCACAGCTCCAGAAGTTGTCTCTAGACAAAGTCGTGACCCAATTTACTCTGGTAAGGGCAGA AACGGTTCACATTCCATTATTTGTAAAGTTACCTGCTGTTTGCTTTCATTATTTTTGCTACACATTTTAT TTGTATTTAAATGTTTTAGGCAATCTAAGAACAAATGTAAAAGTAAAGATGCAGTAAAAACGAGTTGCTT GGTGTGCCCGGCTCCATACGTATCAAGCACAGCGGTAAACAAAATCCCATGTATTTAACTTTTTCTTTCT TTTTTTTTTTTTTTTAAAGTTTTTTGTTTTGTTTTGTTTTTGCTTTTGTGATTTTTTTTTTTTCTTTTTG ATACTTGCCTAACATGCATGTGCTGTAAAAATAGTTAACAGGGAAATAACTTGAGATGACGGCTAGCTTT GTTTTAATGTCTTAAGAAATTTTCATGAACAATCCAAGCATAATTGTTAAGAACACATGTATTAAAGTTC ATGTAAGTGGAATAAAAGTTTTATGAATGGACTTTTCAACTACTTTTCTCTACAGCTTTTCATGTAAATT AGTCTTTTGGTTTTGAGACTTCTCCAAAGGAAATTGTCTCCTTATTCCCTCTTGGCAGCTAATGGGCTTT TACCAATTTTGAATGCAGAGTTTATCATAAAAAAAAAAAAACCGCTAATGTAGCTTCCATCCTCAGCCAC CTCCCACATTTTCCCCTTACTCCTCAACAATTGAAGTGGACTTTTATGGGGTGGGGGGTGATTGGCAAAA GGTAATGTGTTCCATTTATAATTTTGGTCTATGGAGTTTTCTAACTTAGGAAGCCACAATGTTCTTGGCC CATGTGACTGACACTGGGCAGCATTAACTGTTAAGTTCTGTGCTTCCAAGTCGCTGTTGGTTTTAAGAAT TTCCTGATGCTCTTACAGTCTGCCTGGGATTCTATTCCTCTATTTCCATGTTGGGCACAGGCTCACTTCC CTTTTACTGTCTTGTCACCAACCATTCCAACTTGGTGGCCATGTATTTGGGAAAAGGGCCGCATGATCTT TCTGGCTCCACTCAGTGTCTAAGATACCCGCTTCCCCTGCTTGCCTCCCACAGCCTCCCCTCATCCTCTC TACAGCCCAGCTCGCCTCAGTTGAGCTTGTGTTTATCTCCCTGGAAGTCCTGCCCTAAATGGTCTGTCAC CGTCTCCCTTTAAAATCCTTCCTCCTCCTCTTCCTCCTCCTCCTCCTCCTCACATAATGATGGGGCTAAG TCACACCCAAAGCTCACACCCTACCGAGTATTTCCTCAGTACTTTACAGAAAACACCAAACAAAAATGCC ATTTTACAAGAGGTGTATTTTTTTTTCTTTTAGAATGTAAGCTCGTCTAGAGCAGGGACAATGTTTTCTG TATGTTCTATTGTGCCTAGTACACTGTAAATGCTCAATAAATATTGATGATGGGAAAAAAAAAAAAAAAA AAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_011740
• Insert Size: 738 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050891, AAH50891
• RefSeq Size: 3019 bp
• RefSeq ORF: 738 bp
• Locus ID: 22631
• UniProt ID: P63101
• Cytogenetics: 15 B3.1
• Gene Summary: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (By similarity). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1-3 and 5 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.