Dkk3 (NM_015814) Mouse Untagged Clone

CAT#: MC202608

Dkk3 (untagged) - Mouse dickkopf homolog 3 (Xenopus laevis) (Dkk3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dkk3
• Synonyms: AW061014; C87148; dkk-3; mDkk-3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050934 sequence for NM_015814
  CGCGGAACAAACATGCAGCGGCTCGGGGGTATTTTGCTGTGTACACTGCTGGCGGCGGCGGTCCCCACTG CTCCTGCTCCTTCCCCGACGGTCACTTGGACTCCGGCGGAGCCGGGCCCAGCTCTCAACTACCCTCAGGA GGAAGCTACGCTCAATGAGATGTTTCGAGAGGTGGAGGAGCTGATGGAAGACACTCAGCACAAACTGCGC AGTGCCGTGGAGGAGATGGAGGCGGAAGAAGCAGCTGCTAAAACGTCCTCTGAGGTGAACCTGGCAAGCT TACCTCCCAACTATCACAATGAGACCAGCACGGAGACCAGGGTGGGAAATAACACAGTCCATGTGCACCA GGAAGTTCACAAGATAACCAACAACCAGAGTGGACAGGTGGTCTTTTCTGAGACAGTCATTACATCTGTA GGGGATGAAGAAGGCAAGAGGAGCCATGAATGTATCATTGATGAAGACTGTGGGCCCACCAGGTACTGCC AGTTCTCCAGCTTCAAGTACACCTGCCAGCCATGCCGGGACCAGCAGATGCTATGCACCCGAGACAGTGA GTGCTGTGGAGACCAGCTGTGTGCCTGGGGTCACTGCACCCAAAAGGCCACCAAAGGTGGCAATGGGACC ATCTGTGACAACCAGAGGGATTGCCAGCCTGGCCTGTGTTGTGCCTTCCAAAGAGGCCTGCTGTTCCCCG TGTGCACACCCCTGCCCGTGGAGGGAGAGCTCTGCCATGACCCCACCAGCCAGCTGCTGGATCTCATCAC CTGGGAACTGGAGCCTGAAGGAGCTTTGGACCGATGCCCCTGCGCCAGTGGCCTCCTATGCCAGCCACAC AGCCACAGTCTGGTGTACATGTGCAAGCCAGCCTTCGTGGGCAGCCATGACCACAGTGAGGAGAGCCAGC TGCCCAGGGAGGCCCCGGATGAGTACGAAGATGTTGGCTTCATAGGGGAAGTGCGCCAGGAGCTGGAAGA CCTGGAGCGGAGCCTAGCCCAGGAGATGGCATTTGAGGGGCCTGCCCCTGTGGAGTCACTAGGCGGAGAG GAGGAGATTTAGGCCCAGACCCAGCTGAGTCACTGGTAGATGTGCAATAGAAATGGCTAATTTATTTTCC CAGGAGTGTCCCCAAGTGTGGAATGGCCGCAGCTCCTTCCCAGTAGCTTTTCCTCTGGCTTGACAAGGTA CAGTGCAGTACATTTCTTCCAGCCGCCCTGCTTCTCTGACTTGGGAAAGACAGGCATGGCGGGTAAGGGC AGCGGTGAGTCGTCCCTCGCTGTTGCTAGAAACGCTGTCTTGTTCTTCATGGATGGAAGATTTGTTTGAA GGGAGAGGATGGGAAGGGGTGAAGTCTGCTCATGATGGATTTGGGGGATACAGGGAGGAGGATGCCTGCC TTGCAGACGTGGACTTGGCAAAATGTAACCTTTGCTTTTGTCTTGCGCCGCTCCCATGGGCTGAGGCAGT GGCTACACAAGAGCTATGCTGCTCTGTGGCCTCCCACATATTCATCCCTGTGTTTCAGCTCCTACCTCAC TGTCAGCACAGCCCTTCATAGCCACGCCCCCTCTTGCTCACCACAGCCTAGGAGGGGACCAGAGGGGACT TCTCTCAGAGCCCCATGCTCTCTCTCTCAACCCCATACCAGCCTCTGTGCCAGCGACAGTCCTTCCAAAT GGAGGGAGTGAAATCCTTTGGTTTTATTATTTTCTCCTTCAAGGCACGCCTGCCACTAAGGTCAGGCTGA CTTGCATGTCCCTCTAACGTTCGTAGCAGTGTGGTGGACACTGTCTTCCACCGACTGCTTCAATACCTCT GAAAGCCAGTGCTCGGAGTGCAGTTCGTGTAAATTAATTTGCAGGAAGTATACTTGGCTAATTGTAGGGC TAGGATTGTGAATGAAATTTGCAAAGTCGCTTAGCAACAATGGAAAGCCTTTCTCAGTCACACCGAGAAG TCACAACCAAGCCAGGTTGTGTAGAGTACAGCTGTGACATACAGACAGAAGAAGGCTGGGCTGGATGTCA GGCCTCAGATGACGGTTTCAGGTGCCAGGAACTATTACCATTCTGTATCTATCCAGAGTTATTAAAATTG AAAGTTGCACACATTTGTATAAGCATGCCTTTCTCCTGAGTTTTAAATTATATGTATACACAAACATGTG GCCCTCAAAGATCATGCACAAACCACTACTCTTTGCTAATTCTTGGACTTTTCTCTTTGATTTTCAATAA ATACAAATCCCCTTCATGCAAAAAAATTAAAACAATCTGTAGTATAAAGAGACAAAAAAATTCCATAGAA GCAGATTTTCCAGGCATCTGCAGTTTCCCTCTTTTAGAATCGGAATTCGTTGGAACTCTCATCCTTGTCT GGATGGGAATTAGCTTTAACAGAGAAACTACTTCACCCTCTCCTGAAAGAACAAATGGAATATATGAGTC TTCTCTTGGAGGCTCTTTCCACTCAAATGCAGTTCTGGGGCTGTGCTAGCATTGATACTGTAACAAAACG GCTGAAGCAATGAACTTATATATTTAAAAAGTTAGGTTAATTGGGTTCACCATTTCAGGTTTCAGTCCTG ATCCCATGGGGTTGAAACTAAGGAGAGGCAGCACAGCGTGGCAAGGGAATGTGGTAGAGTCAAGCTGCTC CCTTTCTGGCTAACAGGAGAGTGGGCAATGTGCAGTCTTGTGAGAATGCCCAGGTCCTGGGGGGAAGGGA GTGCCCTGGACATCACCTTAAAGGTGGAGACTTCTGCAGCTTTGGTTTTAGTTACTCTTCTGGGTGCTAC AATCAAACGCCCAACAAGAAGCCACCTGAGGGATGAGGGTTTATTTTGGCTCCTGGTTCAAGCAGGGAGT CCTTCGTGGCAGGAGTGCAAGGTTGCTTCCTGCAGTGTGGAGGATCAGGAAGCAAAGAAAGAGCAATGCA AGACTCAGCTTTCTCTCTTTCCCTGATTATTTATTCTGGAACCCCAACCCTTGGGGTGGTGCCGACCGCA GTAAGAGTGAGTGTCCTTTCCTTAGAACCCTCTGAAAACTCTTGGCCTCATAGAAATGTGCAGAGGTGTG TCACCTAAATTGTTCAAATCCATTCTGTTCCAAGACATGGGAGCGCTATGTGCTAAGTCTTCCACATAAG AGCACCGAGTACCTCTTAAACGCCTGTAAATCGCATCTGAAGATACCACAGTAAAGAGATGTAAACATTT AGGAAAACAATAAATGTAACTGATGAAGTCACCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_015814
• Insert Size: 1050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050934, AAH50934
• RefSeq Size: 3290 bp
• RefSeq ORF: 1050 bp
• Locus ID: 50781
• UniProt ID: Q9QUN9
• Cytogenetics: 7 F1
• Gene Summary: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.