Gid4 (NM_025757) Mouse Untagged Clone

CAT#: MC202558

Gid4 (untagged) - Mouse RIKEN cDNA 4933439F18 gene (4933439F18Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gid4
• Synonyms: 4933439F18Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052418 sequence for NM_025757
  AGCGCGCGCGTGTGTGTGTGAGCGCGTGTGTATGAGTGAGTGAGTGTGTATGCGCGTGTGTGTGTGTGTG CGTGCGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACCCGCGAGGGCAAGTGGGAA GGGAGACCCAGCTCAGGTCTGGGAGGCCCTGCTTGCAGGTCTCCGGGTCCAGGTGGCAACACGAGCGCTT GGTCTGGCCTCAGCGGGCGGGTGGTTCCCCGGCCCCTCCCCCGCGCGTGCGCGCCCGGGTCCGCCCTCCC CGCAGCTTCGGCTCCCGCGCGGCGGCGGCGGTTCCTTCCCCCTCCCCCAGCCCTGGCTCCGGGGGACCCC GCGATGCCGGTCCGCACCGAGTGTCCCCCGCCGGCGGGTGCCTCAACCACATCCGCGGCCTCACTCATCC CGCCGCCGCCCATCAACACCCAGCAGCCCGGCGTGGCCACCAGCTTGCTCTACAGCGGCTCCAAGTTCCG TGGCCACCAGAAGAGCAAGGGGAACTCGTACGACGTAGAGGTGGTGCTGCAGCACGTGGACACTGGGAAC TCTTACCTTTGTGGGTACCTAAAGATTAAAGGCCTTACAGAGGAATATCCAACCCTTACAACCTTCTTTG AAGGAGAAATAATAAGCAAAAAACACCCTTTCTTAACTCGGAAGTGGGACGCAGATGAAGACGTGGATCG GAAACACTGGGGCAAGTTTCTGGCTTTTTATCAGTATGCAGAATCATTTAATTCAGACGACTTTGATTAT GAAGAACTGAAAAATGGGGATTATGTCTTCATGAGGTGGAAGGAGCAGTTCCTAGTCCCGGACCACACAA TCAAAGACATCAGCGGTGCTTCCTTTGCTGGTTTCTATTACATCTGCTTTCAGAAGTCAGCAGCCTCCAT AGAGGGCTACTACTACCATCGGAGCTCAGAATGGTATCAGTCCCTCAACCTGACCCATGTTCCTGAACAC AGTGCACCCATCTATGAGTTCCGGTGACAGAGGTTCAGAACAGTGGCCAAATAAAACTGAACTTGGCAAC AAAGAGCTTTGCGAGGAAATTATGTACCTGCCAGAACCAGGAGAGGTGTGTTCCTGTTTGTTCACGAGCA GACTTCATCAGAAAGCATGAATGTTAACCTACAGGATCCAAGGAGCATGGCCAACCTGCAGGCAGGCAGA GGGAGCTGTTCACCTCCCACCCTCCTTCCTCTCAGTGGCATTGTGGTCACCATCTGTTTCTGAACAACCT TAAATGCACTTTCCTTCCTGCACAGCTAACTTCTGCATCACTGAAATGCCATCTTTCCTCGGCCTGCCTC TAGCCAAGTGGAAGGTCAGCTCCTGGGTCACCTTAGCCTTGGTGCTCAGTGGTCTCCACCCAGGCCCTAC TCCTTCCTCAGTTGCTTGTTTGGTAGCCCAAGGAAAGGGAGAGCCCAGCATCTTGTGCCCCAGAGCTCTA TGTGAGTTGCCTGGGCCCAGCATATACCACCAATGAGATGAGATGGGGTCCCTGGTGGGCCTCATTGACC TTTGATTCCAGTTGATGTCTTGTAGGGCCCTGTGTGTTAACAATGCAAGACTAGCACATGGCTGTCGGGT TTTCTGGGCCTGCTGTTGGGGATCACTAAATTTAAGGCTTCCAGATCCCTGGTAGGAGTAGATCCCAAAC TGCTTTCTCAGTACATTTGCTCCAAACTTCTGAACTTGAGGGCAATACTAAATAAAAGAGTTTTTTATTA CAAAATTATTATTTTTATGGTACCTTTAACAAGGACCCTATGGCAAATGCACAATTATTCCGAATTACAT TTATCGTTTTCACATTGAAAACAGCAAATGTTGACGTAGTGACTTTTATATCTATGTCTATATGTATATT TAATCAACCAGCAGTTTTGAAACAGGTCATCCTGGTACAGAAGTTTTGCAGCATTGCTTAAATCACAGTG CTAAACACAGGAACTGTTTCCGGGCCGGCTGAGCATTTGCGCCTCCTCCTTTTGCTACTCAAAACATCAG TGTGTCATGACAACCTTTTCCAAAGGCAGAGGGGGCCCCCATTCTCCGGAAAGGATGACTGAAATGTTGT CATGGCGTGGGCAGGAAGAAATGCACTCAAATAACTCGAGGCTCATGTGCCAAAGTGTGAGAGAGGGGAG TGGTTTGGTTTGGTTTGGTTTGGTTTTTCTAAATGTTTAAAAAGCTTAATAGATTGGCATCAGTTGTAGT CCACAATATTGTCAGGACTTTGGGGAATTCTGGTGGTTTACAGGTCCAAACACAAGGGGAAAAGCTTTAA AATAAATACAGCATCAGGGCTGGGAGTGCAGCTGACCAAGCACTAGCTTGTCACATATGAGGAAGGACCT GGCTTCCATTTCCAGTGCTGGAAAAAGGGAACACCAAGGGGTAGCAGGTTGGCTGCTGTTGTATTGTGCT GTGTGTTACTAATACTGAATATCTGGGAACCTTAAGAGAGCAGAAGTGGGGAAAGAAACCAGCAGGTACT TGGCAATATCCATAGCCCCAAAACTTACCCAGTCAGTAAAGCTGGGGCTCAGAAACCATAACAATACATC ATCCCAAGACTTAGGTAGAAGAAAAGAATCCACATCCATCGCTAGGGCTGAATCAGTGGTTCCTTACCCT TGAGAAGCAGCCTGGGTACGGCAGTGCACCTCGAGGCCCAGCTGCTCAGAAGCCTGAGCCCAGGAGAAAG GGTCAGTCTAAGGTGAACCTAAACTTCATCTGACATCAAAACTCAACTGAATATGAGATGGCCGGCTCTG GTCAGAAGCACCTGGCTTACACCACAGGCCACCATCGCTTCACTTAAGCTGAGGGGCAGCACTGGAGAGT GAAGGAGGCAGAAAGACTGACTGCTGGCTTTTCCCTGCAGCACCTGCTCCTCTGGCTTTCCACCATGCTA TTGAACCATTGTGTGCTGGGCAGGTATCATTCAGCAGTGGATGGAGTCACCCTGAAGCTAGGGCTCGTGT GTAGCTCTAGTAGCACGTCAGTGAACAGCCTCTCCTCTAAACTGGGCAGGCCAGGTCGGAGCATAGGGGT TCCCCCTACACCCCCCTTTTGTTCCTTTGATCTCTTTGTCTTCAGGGTCTCACTTTGTAACTCTAGCTGG CCTGGAGCTCACTTTGTAGATCAGGCTGACCTTGAACTCACTGAGATCCACCTGACTACTCAGAGCGAGT ACTAGGACTAAAGGTGTGAGCCACCACACCCTGCTTTTTTTTTTTTTTTTTTTCTTAGCTAGGGAAGTGT TCCAGCTCTACTTAGAAAGATGAGGGAAGGATGCCTTTAGCAGACAGACTTCAGACCTACCTGAAGACTT GCTGCTTAGAAGTCTGCCCCTGAACACTGGGCAGGAATAAACATGTTCCACTTCTTAGTAACTCCTAGCT GGTGGACAGTGACTTCTTTTCCTTTTCTGTCCCTGGAGGACCTCTGGGACTTGTCTCCATTGGTAGCTCT TTAACCAGCTGCTCAGTGGCTTGCATGCCTCTGGTCCTGTGTGCAATATCTTTGCCTGGCACTTGTATCT AACATTGAAAAATAGGTGCAGACTGCAAATGTCCTACTGTTCTCTACTCCAACGAGCCAACCCCCAGGCA GGGACTAGAGGTCTGGCTGCTCTTCTCTTACCCTCCACTCAGAAGGCTCCACTGCTGTGGCCAGCTGTGG CCCTCTCGTTCCCTGCTTTACCAGTCTACCCACCTATCTCCTTTAAGATGGCCTAGCATGTCAGCTGCAG TCCAAAAGTAAGTAGACAAAGCCTCATGTTAAGTAGACAGTCAGGGTGGACTGGCCCTGTTAGCAGTGTT GGAGTCAGATTCCAGTGGAACAAAGGGGTTTCTCAATGTCTTTTGCTTTCAGAAGCAGGGAATTCATATT CCCTTTAAGGCCTTGAATGGATTTTTTTTCCTCATAAAATAGTCACTGATAAGCTAATTTTTAAAAAGAA ATGCCATTACGTATGTTACATTGTGGTTTAAAATTACTAACAAGTTCTGGGAAAGAAAATACTTGATTTT GAATCTTAAATGTTTTTTAAAAATTAGATTTGAATGGGCGCAAAGTACAAATATTTTGTTTCTTTATGGA AGACCTGTGGAAAGGGTTTGAAGATTTGCAATGGGAGAATTATTTTGCAGAGCTATCAATGTCTAAATAA TTTTTGAAAAAAAAAAACCAATAAAGGTATTTAAGCAGTGTGAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_025757
• Insert Size: 654 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052418, AAH52418
• RefSeq Size: 4264 bp
• RefSeq ORF: 654 bp
• Locus ID: 66771
• UniProt ID: Q9CPY6
• Cytogenetics: 11 B2
• Gene Summary: Substrate-recognition subunit of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Binds proteins and peptides with a Pro/N-degron consisting of an unmodified N-terminal Pro followed by a small residue, and has the highest affinity for the peptide Pro-Gly-Leu-Trp. Binds peptides with an N-terminal sequence of the type Pro-[Ala,Gly]-[Leu,Met,Gln,Ser,Tyr]-[Glu,Gly,His,Ser,Val,Trp,Tyr]. Does not bind peptides with an acetylated N-terminal Pro residue.[UniProtKB/Swiss-Prot Function]