Gid8 (NM_029607) Mouse Untagged Clone

CAT#: MC202405

Gid8 (untagged) - Mouse RIKEN cDNA 2310003C23 gene (2310003C23Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gid8
• Synonyms: 2310003C23Rik; 4833420G11Rik; AI451474; Twa1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC059022 sequence for NM_029607
  GGCTCATCAGTGAACTGCACTTTCTTTGGTGCCAGCTGGAACTGTCTAGGTTGTAGGAGTAGGGAGGAAG GAGGCTTGTGCCATCTTATCAGTTTTCCCACAGAGGAGTTGAAGACTCCACCCTTGACTAACACTTGACT TCCTTTTGATTGCTGTGTCCTTTTTAACACCCTAACATAATGTCTAGTGTATAACTCTATAGTGATTCCC CTTAATACTTTTAGCTTCAAACCTAATCCCCTCCTTCATAGTGTGATGTCTTGGTCTCTTCATTCTAGGA CTTCTGATTCTATTTCACTAACCATACATCCCACCTTTGACCTTGATGCTACCCAGATGTGTAACTGTCT CAGGCCTTTTTTGCTTGTCCGTTTGAAGATTGAAAGCATCCTCCTACTTTTTGGTTTTTGGAGACAGGGC TTCTCTGTATAGCTCTGGCTGGCCTCAAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCG CCTGCCTCTGCCTCCTAAGTACTGAGATTAAAGGCATGTGCCACCACTGTCCGGCCCCTCCTACTCTTAA ACTTACTATATTGCTGTGTTTACTTTTTGAAATGATTAAATTGGACATAGAATTTTTCCCTAACCATTGT ATCGTTTTCCTGTAGAAATAAATCATAATGAGTTATGCAGAAAAGCCGGATGAAATAACCAAAGATGAAT GGATGGAAAAGCTCAATAACTTGCATGTTCAACGAGCAGACATGAACCGACTCATCATGAACTACCTAGT CACAGAGGGCTTTAAGGAAGCAGCAGAGAAATTTCGGATGGAGTCTGGGATCGAACCAAGTGTGGACCTA GAAACACTTGATGAGCGAATCAAGATTCGGGAGATGATTCTGAAGGGTCAGATCCAGGAGGCCATTGCCT TGATCAACAGCCTCCACCCAGAGCTCCTAGACACAAACCGGTATCTTTACTTCCACCTTCAGCAACAACA CTTGATTGAGCTTATCCGTCAGCGTGAGACAGAAGCAGCATTGGAGTTTGCCCAGACACAACTGGCAGAG CAGGGTGAGGAAAGCCGAGAATGCCTCACAGAGATGGAGCGCACACTGGCCTTGCTGGCCTTCGATAGCC CTGAGGAGTCGCCTTTTGGAGACCTCCTTCACATGATGCAGAGGCAAAAGGTATGGAGTGAAGTTAACCA GGCTGTTCTGGATTATGAGAATCGAGAGTCCACACCCAAGCTGGCAAAATTACTGAAACTGCTACTTTGG GCTCAGAATGAGCTAGACCAGAAGAAAGTAAAATATCCCAAAATGACAGACCTCAGCAAAGGTGTGATTG AGGAGCCCAAGTAGAGCCTGTGCATGGACCCAGCACTGTCAATAGGCACAGGACTCTCCCTCCTCCGGCT GTGACTGGCAGATTGTCACCACAGTAGAGAACCTTTCCCCTGTACTTTTCTTTGGCCCCATTGTTTAAAA GGGGAAAATGGCCTTTTTAAACCCTGTCAAGCCCATGATGGAAGACACCACCTCTGCCATGCCCTGTATC CCGAGAGCTGCACACACTGCTTCCAGATTGCTGGTTCCAGAAGATGTAATTTGCCACTGTGTGCTGCACG GCTAGGGAGTGTCATCTCAGCTGAAGGCCTCACCCAAGACAGGCAGACAGACATGAACATTACTGATGTG CTTTTCCGACCTGCTATGTGCAGGCAGGCAGGGGTTATCTTCCTGGGTCTCACAGACTTTCATGGTTTTC CTTACCCCTTCTTTTCTTTTTCTTCCTCATTTTGTTCCTTCATAGGAAAAAAATGTATAAATTTGTAAAT CTTAATTCAAAGATAACTTTTGTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_029607
• Insert Size: 687 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059022, AAH59022
• RefSeq Size: 1877 bp
• RefSeq ORF: 687 bp
• Locus ID: 76425
• UniProt ID: Q9D7M1
• Cytogenetics: 2 H4
• Gene Summary: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.