Nubp1 (NM_011955) Mouse Untagged Clone

CAT#: MC202338

Nubp1 (untagged) - Mouse nucleotide binding protein 1 (Nubp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nubp1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055436 sequence for NM_011955
  GAGCGCAGCCCGGGCAAGAGCGGCAGCATGGAGGAGGCGCCCCACGGCTGCCCCGGGGCCGACAGCGCCC AGGCCGGCCGAGGAGCCTCGTGCCAGGGATGCCCCAACCAGAGGCTGTGCGCATCCGGAGCCGGCGCTGC TCCGGATCCCGCTGTGGAGGAAATCAGAGAGAAGATGAAGACGGTGAGACACAAGCTCTTGGTGCTGTCT GGAAAAGGCGGCGTTGGGAAAAGCACGTTCAGTGCACACCTCGCCCACGGCCTGGCGGAGGATGGAGACA CACAGGTCGCTCTTCTGGACATCGATATCTGTGGGCCATCGATTCCCAAGATCATGGGCTTAGAAGGAGA GCAGGTTCACCAGAGCGGCTCCGGCTGGTCTCCAGTGTACGTGGATGACAACCTGGGGGTGATGTCGGTG GGCTTTCTGCTTAGCAGCCCAGATGATGCTGTCATCTGGAGGGGACCAAAGAAAAATGGTATGATCAAGC AGTTCCTCCGTGATGTAGACTGGGGAGACGTGGACTACCTCATCATAGACACCCCGCCTGGCACGTCTGA TGAACACCTCTCAGTGGTCCAGTACCTGGCTGCGGCACACATTGATGGGGCAGTGATCCTCACCACCCCT CAGGAGGTGGCCCTCCAGGATGTCCGGAAAGAGATCAGCTTCTGCCACAAGGTGAAGCTGCCCATTATTG GTGTGGTAGAGAACATGAGCGGTTTCATCTGCCCCAAGTGCAAGAAAGAGTCTCAGATCTTCCCTCCCAC GACTGGGGGTGCAGAGGCCATGTGCCAGGATCTGAAGATTCCACTTCTGGGCAAAGTGCCGCTGGATCCA CACATAGGCAAGAGCTGTGACAAAGGCCAGTCTTTTTTTGTCGAAGCTCCAGATTCACCAGCCACAGCAG CCTACAGAAGTATAATTCAGAGGATCCGAGACTTTTGTAATTCTCGTCAGTCACATGCTGAGACCCTCAT CAGTCCCTGAAGTGGAACAGGACCAAGTGCCACCTGCTAACCCCAGAGTTGGCAGGCCACGTGTCCAATG GCAGGCCATTGTCTGGGCAATGGGGGACAGGTGGTACCATAGCCACCAGATGCTGACAGGATTTGAAGTC ACTCATTAAGACACTGTAATCACCTATCTGCCTATTTCCTCGAGATCAAAGGTACGGGCGCTCTGCACTC CAGGTGCCATTCATCGGAAAATAAAAATGTCTCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011955
• Insert Size: 963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055436, AAH55436
• RefSeq Size: 1264 bp
• RefSeq ORF: 963 bp
• Locus ID: 26425
• UniProt ID: Q9R060
• Cytogenetics: 16 5.61 cM
• Gene Summary: Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (By similarity). Implicated in the regulation of centrosome duplication (PubMed:16638812, PubMed:23807208). Negatively regulates cilium formation and structure (PubMed:23807208).[UniProtKB/Swiss-Prot Function]