Pias1 (NM_019663) Mouse Untagged Clone

CAT#: MC202291

Pias1 (untagged) - Mouse protein inhibitor of activated STAT 1 (Pias1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pias1
• Synonyms: 2900068C24Rik; Ddxbp1; GBP
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051417 sequence for NM_019663
  GCAAGATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAAGTACT GTTGGGCTACGCTGGGAGGAACAAGCACGGACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTA AAGGCTGGCTGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTTCCCTCAGAAAA TTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTCCTATGCCTCCGACTCTTTCTCCATC CACCATTCCACAGCTCACTTATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGA CCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACCCGGACATAAAGCTGC AGAAGCTACCATTCTATGACCTGTTGGATGAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCA GCGCTTTCGGGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCTCCATG GATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTAAGGTTTTGTTTATCAGAAACCAGTT GTCCACAAGAAGATCACTTCCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGG TTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGACCAATTAATATCACCTCACTT GTCCGATTGTCCACGACAGTACCAAATACCATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATT CCATGGCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAGGGAAT AAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAACTGCAGATCCAGATAGTGAGATAGCT ACTACCAGCCTACGGGTTTCGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCAC TTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGAGAAAAAACCAACATG GGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATT CTAAAGTACTGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAGGTCAA AGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATGGTTGCTTGAGCTCCACATTGGAGCA TCAGGTAGCGTCCCACAACCAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGAC AGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTCCTTCCCTGTCTCCTACGTCAC CACTAAGTAATAAAGGCATTTTAAGTCTTCCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCC TGCTGTAGATACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCCACATGACGCCT ATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCT CCCTGCTAGCCGCAGCTGCAGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCCC GTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGCACATCTCTGCCAGCCACCAAC GGAAGCAGTAGCGGCAGCAACAGCAGCCTTGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTG TGGCCAGCAGGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATCTCATTGGACTG ACGCTCTCACCCTGCTGCTCCCACCCCATCCCAGATTAAATGACTTGGCAGAAGAGAACTCTATGCTCTG TTTTACCTTATCCTGTTTAGAAAAGTATACAAGCGTGGTTTTTTTTTTCCTTTTTTTTAGGGAAAAAAAA ATTAAAAGAAATGTACAGAGAACAAAACTATATTTTCAGTTTTACTTTTGTATATAAATCTAAGACTGCC TGTCTGATAAAACACTTGTTTAAAAAAAAAAAAAAGAAAAAAGAAAAAAGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019663
• Insert Size: 1956 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051417, AAH51417
• RefSeq Size: 2235 bp
• RefSeq ORF: 1956 bp
• Locus ID: 56469
• UniProt ID: O88907
• Cytogenetics: 9 B
• Gene Summary: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA (By similarity). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (PubMed:24061474).[UniProtKB/Swiss-Prot Function]