Nbn (NM_013752) Mouse Untagged Clone

CAT#: MC202265

Nbn (untagged) - Mouse nibrin (Nbn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nbn
• Synonyms: Nbs1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055061 sequence for NM_013752
  CGCACGTCCCGGAGCCCGCGCCGGTGCAGCCCGGTGACCGCGCAGGCTGGGCAGCTGCGGTTGGCGTCGC ACGTCGCGGGGTTCTGAGACGCCGGCCCCATGTGGAAGCTGCTCCCGGCCGCCGGTGCGGCTCCAGGAGA ACCATACCGACTTTTGGCCGGCGTGGAGTACGTTGTTGGGAGGAAAAACTGTGGCATTCTGATTGAAAAT GATCAGTCAATCAGTCGAAACCATGCTGTCTTAACAGTAAACTTCCCTGTAACCAGTTTGAGTCAAACAG ATGAAATTCCTACATTAACAATAAAAGATAATTCTAAGTATGGAACCTTTGTTAATGAAGAAAAAATGCA GACTGGTCTTTCCTGCACGTTGAAGACAGGAGATAGAGTTACCTTTGGGGTGTTTGAAAGTAAATTCAGA GTAGAATACGAGCCCTTGGTTGTTTGTTCTTCTTGTTTAGATGTCTCTGGGAAAACTGTTTTAAATCAAG CTATTTTACAGCTTGGAGGACTTACCGCAAACAACTGGACGGAAGAATGTACCCACCTTGTCATGTCGGC AGTTAAAGTCACCATTAAAACTATATGTGCACTCATTTGTGGACGTCCAATTATAAAACCAGAATACTTT TCTGAATTTCTCAAAGCAGTTGAATCTAAGAAACAGCCTCCAGATATTGAAAGTTTTTACCCACCCATTG ATGAACCAGCTATTGGAAGTAAAAGTGTTGATCTGTCAGGGCGACATGAAAGAAAACAGATCTTCAAAGG AAAAACATTTGTGTTTTTAAATGCCAAGCAGCACAAGAAACTCAGCTCGGCAGTTGCTTTCGGAGGTGGA GAAGCCAGGCTGATGGCAGAAGACGACGAAGAGGAACAGAGCTTCTTTTCAGCTCCCGGAACCTGCGTTG TTGATGTAGGAATAACGAATACACAGCTCATAATTTCACACTCCCAGAAAAAATGGATTCATTTGATAAT GGATACACTTCAAAGGAATGGTCTCAGACCTATTCCTGAAGCGGAGATTGGATTGGCTGTTATTTTTATG ACTACAGAGAATTACTGTAATCCGCAGGGCCAGCCTTGTACAGAATTAAAGACAACGACTCCAGGACCAA GCCTTTCCCAAGTCCTGTCAGCCAATGGAAAAATAATCCCAAGTGCTCCAGTGAATATGACCACATACGT AGCTGACACAGAATCAGAGCCAGCAGATACATGTATGCCTTTGAGTGAAAGACCAGAAGAAGTAAAGATC CCTGGACTGGAACAAAGCTCTAGGAAACTTTCACAAGAAACATTCAATATAAAGGAGGCCCCTAAACCAA GCTCCAAAGCTAACAACGTAGCATCAGATACGCTGGTTAGAGGAAAGACCCCGAGCTATCAGCTTTCTCC AATGAAATTTCCTGTTGCAAATAAAAATAAGGATTGGACTTCTCAGCAGCAGCAGAACTCCATCAAAAAC TACTTCCAGCCATGCACCAGAAAAAGGGAAAGGGATGAAGACAACCCAGAGCTGTCCTCGTGCAAATCAT CCAGGATGGAGCTGTCCTGTTCTCTTTTAGAACAAACACAACCTGCCGGACCCTCACTGTGGAAAAGCAA GGAGCATCAGTCTCAGAATGCGACCCTGGACAGGGAAGCCGACACCTCATCTGTGGGTGGGATGGACATA GAACTCAACAGGAAGAGTCCTGACAGGAAACCACTTCCCACAGAAACTCTTAGACCCAGAAAAAGAAAAG ATGTTGATTTAGCTACAGAAGAGGAAGTCTTGGAAGAGTTACTGAGGAGTACAAAGCCAGAGTTGGCAGT TCAAGTGAAGGTTGAAAAGCAGGAGGCAGATGACACCATCAGAAAAAAGCCAAGGATGGACGCAGAGAGG AATCGGCCCCTGAATGGTGGATCAGAGCCGGAAAGCAACAGCGCTCTTCAAGAAGATGAAAGAGAAAAGA AAGATGAACTTCAGACAGAGTCGTGGTCAACAAAACATGAAATAGCTAATAGTGATGGTCTTCAGGACAG CAGTGAGGAGCTGCCACGGAAACTGCTGCTGACTGAATTTAGGTCATTGGTTGTCAGTAACCACAACTCC ACCTCCAGAAATCTATGTGTAAATGAATGTGGTCCACTGAAGAATTTCAAGAAATTCAAGAAGGCGACAT TTCCTGGGGCAGGAAAGCTTCCACACATCATCGGAGGATCAGACTTAGTAGGCCACCATGCTCGAAAGAA TACCGAGTTAGAAGAATGGTTGAAACAGGAAATGGAGGTACAGAAACAACAAGCAAAGGAGGAATCTCTT GCTGATGATCTGTTTAGATATAATCCTAATGTAAAAAGAAGATAATTGAACCTTGTAAAGGAAGCTGTAG AAACACATTTCTACTTCAGACCAACTGAGATACATTAATATAGTACATAGAAATTATAAATGCTTTAAGG TTTCAGTTTATTAAATAAAGGAAACAAACTCTAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013752
• Insert Size: 2256 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055061, AAH55061
• RefSeq Size: 2503 bp
• RefSeq ORF: 2256 bp
• Locus ID: 27354
• UniProt ID: Q9R207
• Cytogenetics: 4 A2
• Gene Summary: Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex (By similarity).[UniProtKB/Swiss-Prot Function]