Gspt1 (NM_146066) Mouse Untagged Clone

CAT#: MC202205

Gspt1 (untagged) - Mouse G1 to S phase transition 1 (Gspt1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gspt1
• Synonyms: AI314175; AI326449; AV307676; C79774; G1st; Gst-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031640 sequence for NM_146066
  TGGATCCCAGCAGCGGCGGTGGCGGCGGTGGCGGCGGCGGTGGGAGCAGCAGCAGCAGCGACTCTGCGCC CGACTGCTGGGACCAGACAGATATGGAGGCCCCCGGGCCCGGGCCTTGCGCCGGCGGCGGGAGCGGCAGC GGCTCCATGGCCGCGGTGGCCGAGGCCCAGCGTGAGAACCTCAGCGCGGCTTTCAGCCGGCAACTCAACG TCAACGCCAAGCCTTTCGTCCCCAACGTCCACGCCGCTGAATTCGTGCCGTCCTTCCTGCGGGGTCCGGC CCAGCCGCCGCTATCCCCAGCTGGCGCAGCCGGCGGCGACCACGGAGCAGGCAGCGGCGCGGGAGGCCCT TCGGAACCTGTAGAGTCCTCTCAAGACCAGTCGTGTGAAGGTTCAAATTCTACTGTTAGCATGGAACTTT CAGAACCTGTTGTGGAAAATGGAGAGACAGAAATGTCCCCAGAAGAATCATGGGAGCACAAAGAAGAAAT AAGTGAAGCGGAGCCAGGGGGTGGTTCCTCAGGAGATGGAAGGCCACCAGAGGAAAGCACCCAAGAAATG ATGGAGGAGGAGGAAGAAATACCGAAACCTAAGTCAGCGGTGGCACCACCAGGTGCTCCCAAGAAGGAAC ATGTAAATGTAGTGTTCATCGGACATGTTGATGCAGGCAAGTCAACCATTGGAGGACAAATAATGTATTT GACTGGAATGGTCGACAAAAGGACACTTGAGAAGTATGAACGAGAAGCTAAAGAAAAAAACAGAGAAACT TGGTACTTGTCTTGGGCCTTAGACACGAATCAGGAAGAACGAGACAAGGGCAAAACCGTGGAGGTGGGTC GTGCCTATTTTGAAACGGAAAAGAAGCATTTTACAATTCTAGATGCTCCTGGCCATAAGAGTTTTGTCCC AAATATGATTGGTGGTGCCTCTCAAGCTGACTTGGCTGTACTGGTAATCTCAGCCAGGAAAGGAGAGTTT GAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCAAAGACAGCAGGTGTCAAAC ACTTAATTGTGCTTATTAATAAGATGGATGATCCAACGGTAAACTGGAGCAACGAGAGATATGAAGAATG CAAAGAGAAACTAGTACCATTTTTGAAAAAAGTTGGCTTCAATCCCAAAAAGGACATTCATTTTATGCCG TGCTCAGGACTGACAGGAGCGAATCTTAAAGAACAATCAGATTTCTGTCCTTGGTACATTGGCTTACCAT TTATTCCATATCTGGATAATTTGCCAAACTTCAATAGATCAGTTGATGGACCAATCAGGCTGCCAATTGT CGATAAGTACAAGGATATGGGGACTGTGGTCCTGGGAAAGCTGGAATCTGGATCTATTTGTAAAGGCCAG CAGCTTGTAATGATGCCAAATAAGCACAACGTGGAAGTTCTTGGAATACTTTCTGATGATGTAGAAACGG ACTCTGTAGCTCCAGGTGAAAACCTCAAAATCAGACTGAAAGGAATTGAAGAGGAAGAGATTCTTCCAGG ATTCATCCTTTGTGATCTTAATAATCTTTGTCATTCTGGACGCACATTTGATGCTCAGATAGTGATTATA GAGCACAAGTCCATCATCTGCCCAGGGTATAATGCGGTGCTGCATATTCATACCTGTATCGAAGAAGTCG AAATAACAGCCTTAATCTGCTTGGTAGACAAGAAATCAGGAGAGAAAAGTAAGACTCGACCCCGTTTTGT AAAACAAGATCAAGTATGCATTGCGCGTTTAAGGACAGCAGGAACCATCTGCCTGGAGACCTTTAAGGAC TTCCCTCAGATGGGACGTTTTACCTTAAGAGATGAGGGTAAGACCATTGCAATTGGAAAAGTTCTGAAAC TGGTTCCAGAGAAAGACTAAGCATTTTCTTGATGACCCTGCACAGTACTGTGAGGAAAATTGACTGCAAA AGCCTACTTCACACCGCCTTCTCTTACTTTCTGCCCATTGACAAACCTCTCCCCATACTTTGCAAAGAAA ATTCACAGCAAAAGTCCACATTATGTCAGCTTTCTCATATTGAGAGCTCTGCTATGCCACTGTTGAATTT TCCCAAGATTTTGCCCCCCTTTCAAACTTCGCTTCCTTGGACAGATTTGGCAATAGCTTTGTAAGTGATG TGGACATAATTGCCTACAATAATGAAAACCTACAGGAATTTTTTTATTTTTCATTTTCTTCTTAGGCATA GTTAGTCTTTTCCCCCCAGGCAGATGATTCTGAGTGTGGGAGTGTGTCTGCACATGTTAAAAAGACAACT ACCATATTAATAAAATATTCAATTTGAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_146066
• Insert Size: 1764 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031640, AAH31640
• RefSeq Size: 2359 bp
• RefSeq ORF: 1764 bp
• Locus ID: 14852
• UniProt ID: Q8R050
• Cytogenetics: 16 6.38 cM
• Gene Summary: Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth (By similarity). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.