Adhfe1 (NM_175236) Mouse Untagged Clone

CAT#: MC202122

Adhfe1 (untagged) - Mouse alcohol dehydrogenase, iron containing, 1 (Adhfe1), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adhfe1
• Synonyms: 6330565B14Rik; Adh8; AI043035; HOT
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026584 sequence for NM_175236
  CTGCCATGGCTGCAGCTGCACGCGCTCGAGTCACACACTTGCTGAGGCACCTACAAAGCACAGCATGCCA GTGCCCAACTCATTCTCATACTTACTCCCAAGCCCCTGGACCTTCGGGAAAAACAGCAGATTACGCCTTT GAGATGGCTGTCTCAAATATTAGATATGGAGCAGGGGTTACGAAGGAAGTAGGCATGGATCTACAGAATA TGGGAGCTAAGAATGTTTGCTTGATGACAGACAAGAACCTCTCCCAGCTCCCTCCTGTACAAATAGTTAT GGATTCCCTATCGAAGAATGGCATCAGTTTTCAGGTTTATGATGATGTAAGGGTGGAACCTACAGATGGA AGCTTCATGGATGCAATTGAATTTGCCAAAAAGGGAGCGTTTGATGCCTATGTTGCTGTGGGTGGGGGCT CCACCATGGACACCTGTAAAGCCGCCAATCTGTATGCATCCAGCCCTCACTCTGAGTTCCTTGATTATGT CAATGCCCCCATTGGGAAGGGGAAGCCAGTAACTGTGCCTCTTAAACCTCTGATTGCAGTTCCAACTACC TCAGGGACTGGCAGTGAGACTACCGGGGTTGCCATTTTTGACTATGAGCATTTGAAAGTAAAAACTGGCA TTGCTTCACGAGCCATCAAACCCACCCTGGGACTGGTTGATCCCCTGCACACTCTCCACATGCCTTGCCA GGTGGTTGCCAACAGTGGCTTCGATGTCCTTTGCCATGCTCTGGAGTCATACACCGCCATTCCCTACAGC ATGCGGAGCCCCTGCCCTTCCAACCCCATCCAACGACCAGCATACCAGGGTAGCAACCCAATCAGCGACA TCTGGGCAGTCCATGCACTGCAGATTGTTGCCAAATATCTGAAGAGGGCTGTCAGAAACCCAGATGATCT GGAAGCAAGGTCTAAAATGCACTTGGCAAGTGCTTTTGCTGGCATTGGCTTCGGAAATGCTGGTGTTCAT CTGTGCCATGGCATGTCTTACCCAATTTCAGGTTTAGTGAAGACATATAAAGCCAAGGAATATAATGTGG ATCACCCTCTTGTGCCCCATGGCCTTTCTGTGGTGCTCACCTCTCCTGCAGTGTTCACCTTCACAGCCCA GATGTTTCCAGAGCGGCACCTGGAGACGGCAGGAATATTAGGAGCCAACATCCGCACTGCCAGGATCCAA GATGCTGGGCTTGTGTTGGCAGATGCCCTCCGAAAATTCCTATTTGACCTAAATGTTGATGACGGTCTGG CTGCTCTTGGTTATTCCAAGGATGACATTCCTTCATTGGTGAAAGGAACACTGCCCCAGGAAAGGGTCAC GAAGCTCGCACCTCGTGCCCAGTCAGAAGAGGATTTGTCTGCTTTGTTTGAAGCATCCATGAAACTGTAC TAATTAACATATTAGTGAAAAGAATCCCCATTGGCCTTTGCGACAGAAGTTACAAATAGCAGAAGTTGGC CAGAGCTGATGTTGCCGTCTTATCTCCACACTTAACTCACCTATTGCTGGTTTGAATGTGATGTGATTTT ATCCTTTGGAAGATTCCTTGGTGCTCATTACATGGAACCCATGGGCCATACCCCAGGGGTCAGTATTCCT GTACCAAACCCCATCAAATACATAAAAATGCCTATCTCCAAACACTGCATGTCTAGACATTTACCCTGAA GAAATACTAGCATTAGTGTGCAAAAAAAAAACAATGCATACTAGGTTGCACACAAGGATGAGTCTGACAT ATTTTTAGATAGTAAAAATTAAAACAATCTAAATATTCAATACTGAATGAATCCTAATATAGTAAATGAT AGAATACTGTGGTATTATTAATAAAAATGAAGTAAAACTCTTGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_175236
• Insert Size: 1398 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026584, AAH26584
• RefSeq Size: 1878 bp
• RefSeq ORF: 1398 bp
• Locus ID: 76187
• UniProt ID: Q8R0N6
• Cytogenetics: 1 A2
• Gene Summary: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.