Ercc3 (NM_133658) Mouse Untagged Clone

CAT#: MC202117

Ercc3 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ercc3
• Synonyms: BTF2 p89; Ercc-3; XPB
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026575 sequence for NM_133658
  GAGGAGGTGGTGCGGTGTGCCGGCCTGCCTGCATCTAGAGCTGTTATGGGCAAAAGAGATCGAGTGGACC GCGACAAAAAGAAATCCAAGAAGAGGCAGTATGAAGAGGAAGAGGAAGACGAAGACGACATTCCTGGGAA CGAGTCTCAGGAGGCGGTGCCCTCCGCCGCTGGGAAACAGGTGGACGAGTCCAGCACCAAAGTGGATGAG TATGGAGCAAAGGACTACAGACAGCAGATGCCACTAAAGGGTGACCATACCTCCAGGCCCCTCTGGGTGG CTCCTGATGGCCACATTTTCTTGGAAGCCTTCTCTCCAGTTTACAAATATGCCCAAGACTTCCTGGTGGC AATTGCAGAGCCGGTGTGCCGGCCTACTCATGTACATGAATACAAGCTAACCGCCTACTCCCTCTATGCA GCGGTCAGTGTTGGGCTGCAAACCAGTGACATTACTGAGTACCTCAGAAAGCTCAGTAAGACTGGAGTTC CTGATGGAATTATCCAGTTTATTAAGCTGTGCACTGTCAGCTATGGGAAGGTCAAGCTGGTCCTCAAGCA CAACAGGTACTTTGTTGAAAGTTCCCACCCTGATGTTATCCAGCATCTTCTTCAAGACCCAGTGATCCGG GAATGTCGCTTGAGGAACGCCGAGGGCGAGGCCACCGAACTCATCACAGAGACTTTTACAAGCAAATCTG CTATTTCTAAGACGGCGGCGGAAGGCAGTGGCGGGCCTTCTACTTCGCAGGGGGTAGATGCACAGGCCAC GTCTGACATACCCAAAGACCTGTTTGATTTTTATGAGCAAATGGACAAGGATGAGGAGGAGGAGGAAGAG ACACAGACAGTGTCCTTTGAAGTTAAGCAGGAAATGATCGAGGAGCTGCAGAAGCGCTGCATCTGCTTAG AGTACCCGCTGCTGGCAGAGTATGACTTCCGGAATGACACTCTCAATCCTGACATCAACATTGACCTGAA GCCCACAGCCGTACTCAGACCCTATCAGGAGAAGAGCCTGCGGAAGATGTTTGGGAACGGACGAGCACGC TCAGGAGTCATTGTTCTTCCCTGTGGTGCTGGGAAGTCCCTGGTCGGCGTGACTGCCGCATGCACTGTCA GAAAGCGCTGTCTCGTCCTGGGCAACTCGGCTGTGTCTGTGGAGCAGTGGAAAGCCCAGTTTAAGATGTG GTCAACCATCGATGACAGCCAGATCTGCCGCTTCACCTCAGATGCCAAGGACAAGCCCATCGGCTGCTCC GTTGCCATTAGCACTTACTCTATGCTGGGCCACACCACCAAAAGGTCCTGGGAAGCTGAGAGAGTCATGG AGTGGCTCAAAACCCAGGAGTGGGGCCTCATGATCCTTGACGAGGTGCACACCATTCCAGCCAGGATGTT CCGGCGGGTTCTGACTATTGTGCAGGCGCACTGTAAGCTTGGTTTGACTGCAACCCTTGTCCGGGAAGAT GACAAAATTGTTGACTTAAATTTCCTGATTGGGCCCAAGCTCTATGAAGCCAACTGGATGGAGCTGCAGA ACAATGGGTACATCGCTAAAGTCCAGTGTGCTGAGGTTTGGTGCCCGATGTCTCCCGAGTTCTACCGAGA GTATGTGGCAATCAAAACAAAGAAACGGATCCTGTTGTACACCATGAATCCCAACAAATTCAGAGCCTGC CAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATTGTCTTTGCTGACAACGTATTTGCCT TGAAGGAATATGCCATTCGGCTGAACAAACCTTATATCTACGGGCCCACGTCCCAGGGAGAACGTATGCA GATTCTCCAGAACTTCAAGCACAACCCCAAAATCAACACCATCTTCATCTCCAAGGTTGGTGACACATCC TTTGATCTGCCAGAAGCCAATGTCCTCATTCAGATCTCTTCCCATGGTGGCTCCCGACGGCAGGAAGCCC AGAGACTGGGACGGGTACTCAGAGCCAAGAAAGGGATGGTCGCAGAGGAATACAATGCCTTTTTCTACTC CCTGGTATCTCAGGACACACAGGAAATGGCTTATTCTACCAAGCGACAGAGATTCCTAGTGGATCAAGGT TACAGCTTTAAGGTAATCACAAAGCTAGCTGGCATGGAGGAAGAGGAGCTGGCGTTCTCCACCAAAGAGG AGCAGCAGCAGCTCCTGCAGAAGGTGCTGGCAGCCACCGACTTGGATGCAGAGGAGGAAGTGGTGGCTGG AGAGTTTGGCTCTAGATCTGGCCAGGCATCCCGACGCTGTGGCACCATGAGCTCCCTGTCAGGCGCAGAC GACACCGTGTACATGGAGTACCACTCCTCCCGAAGCAAGGCCTCCAGCAAGCACGTGCACCCGCTTTTCA AACGCTTCAGGAAGTGACGCCCACCCTGTGCTCAGGTGAAGACTGGCTGCCTGGATCCGTGTTGAAGAGA GACTTCTTTCTTCACCGGTGTGATCGTTTCTATCCAAGACTGTGTCCTTTCCTATTGACTGACTGCTGAA AACCAGTCAGTGTGAGGCCTTAACTGCCTTCGTGGGTCCTAGGTTTAAAAAGGAAAGAAAAGCTACTGTT GTTTTTTATTCAAACTTGTTAAGAGTGAATCATTTCTGTATATAAAAATTATACCACTTAATTATAAAAA AAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133658
• Insert Size: 2352 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026575, AAH26575
• RefSeq Size: 2670 bp
• RefSeq ORF: 2352 bp
• Locus ID: 13872
• UniProt ID: P49135
• Cytogenetics: 18 B1
• Gene Summary: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]