Ptpa (NM_138748) Mouse Untagged Clone

CAT#: MC202081

Ppp2r4 (untagged) - Mouse protein phosphatase 2A, regulatory subunit B (PR 53) (Ppp2r4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptpa
• Synonyms: 2610042B21Rik; C77440; N28142; Ppp2r4; PR53
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052852 sequence for NM_138748
  AAGAGGTAATCCCACCGGCATGGCTGCCGCCTTCGCGCGAGTGACTGTAATTCTCGGCTTCCGGTCCTAG CCAGTCAGCGCTCATTTCCCTGCTGGAAGCTTGGCGGAGCCGGGGAGAGCTGCGGGGTGAACATTGAGAG GGACCGGCGAGCATCCGCTCCGGCTCCTCACGGAGAAGATGGCCGAGGGCGAGCGGCAGCCGCCGCCAGA TTCTTCAGAAGAGACCCCTCCAACTACTCAGAACTTTATCATTCCAAAAAAGGAGATCCACACAGTTCCA GATATGGGCAAATGGAAGCGCTCTCAGGCATATGCTGACTACATTGGCTTCATCCTTACCCTCAATGAAG GTGTGAAGGGGAAGAAGCTGACCTTCGACTACAAAGTCTCTGAGGCCATCGAGAAGCTGGTGGCACTTCT TGATACGCTGGATAGGTGGATTGATGAAACCCCGCCAGTGGACCAGCCTTCCCGGTTTGGGAACAAAGCC TACAGAACCTGGTATGCCAAACTTGATCAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCCACCCACC TGGCTGCTGCTGTGCCTGAAGTGGCAGTTTACCTGAAGGAGGCTGTGGGGAACTCCACACGAATTGACTA TGGCACAGGGCATGAGGCTGCCTTTGCTGCTTTCCTCTGTTGTCTCTGCAAGATTGGTGTACTCCGGGTG GACGACCAGGTGGCTATTGTCTTCAAGGTGTTTGATAGGTATCTTGAGGTTATGCGGAAGTTGCAGAAGA CATACAGGATGGAGCCTGCAGGCAGCCAGGGCGTATGGGGTCTGGATGACTTCCAGTTCCTGCCCTTCAT CTGGGGCAGCTCACAGCTCATAGACCACCCCCACCTGGAGCCCAGACATTTCGTGGATGAGAAGGCGGTG AGCGAGAACCACAAGGACTACATGTTTCTCCAGTGCATCCTGTTCATCACTGAGATGAAGACTGGCCCCT TTGCGGAACACTCCAACCAGCTGTGGAACATCAGTGCTGTCCCCTCCTGGTCTAAAGTGAACCAGGGCCT CATTCGAATGTATAAGGCAGAGTGCCTGGAGAAGTTCCCTGTGATCCAGCACTTCAAGTTCGGGAGCCTG CTGCCCATCCATCCCGTCACATCAGGCTAGGAGAGGCTGAACTACCAGAGCCACCCTGGCCAGGTTCCTG TGCTCTCTGTGACCCAGCACCCCTCCCGCCCACCTTCTGTTCTTTCTGTTTGATGAGAGGCTGTTTACTG AGATGGGTGCTAAGCAGGGCCTGAGTTGGGGGAATTGACCACAGTGTGCCCAGTTTTTGACTGTCCTCCG TCTGGTGGCCGAGTAAGAAGAGAGGGGCCTGGACGTGGTCCCCGGCCCTGCCCATCCCTGTGCTCCTGCT TCCTCCCACGGTCAGGCCCATGTGGAGGAGACAAGGACACCCTGCTCTTGTCATTGCTCCCCCTTTCCTC CCCATGATGTAATGGTTCCCTCATTAAGAGCAGGAGTTCCGTGCCTCCAGGGCCGGCTGGTGGGTTCCCC GCTAGCTGTGTTCCACTGGGTATCTGCTATGGAGCTGAAGGGTGAGGCTGTCATCAGCTGTTGGGAGGGC TGGGGTTGGGGGAGGTTGGTTTGTAGGGGCCATGGATGATGAGGCTGGTCTTCTCAAGGTACCATCCTAG GCCCTGGGGGACCTGTGTCCATCCTCATGCTGGCTTTGTTCCCTGGCCCTTGGCTTTGCTAGGTTTCCTG ACTGTTAACATAACACTCCCCTTCCTGGGGATAGCCTAGCTATGGAAGCTGCCCTGGGCTCCTGGGAAAG CCCATTGCTTGGGTGAGATCTGACTTTCCAGAGCTGTCAAGCAGGGAGAGGAAGAGACCCCGGGAGGCAG TAGGACTGTTGTGTCTGTGGAGAACCTAGTTTTCTCCAGTCAGTCTCAGGCCTGCTGATTCTGCTGCCTC TGCCTGCTGAGTGTCTGAAGCTTCCCCCGAGGCCTTGGCTTGCTGCTCCGCTCCCTTGGGTCGGTTTGTG GCTCGTGTGGGAGAGCAAAGAAGGTAGCAGCCCCCTCCTCCCACTTCCCTCTGTGAGATGAGGGAAGTGC TGGCCTGGGCCTAGGCATAGACTCAGAATGGGTAGGGCTGCTAAGGGGACCTAGCCACAGTCACCAGTTT TCTCTCAGCGTGTGGCTGAACTGGAACCTGCAGGCTTTCCTGTCCCACTTGGGCCTGCCCTTCTTCACTG CCCATCCTGTGTTTGTGCCCGCTTAGCTTTGTGGAGTGGACACTGAGAAGCACAACTCAGGGGCTTGGCC AGGAATTAACTATGGTCTGGGCAGAGCGGGGATTGAAGTCATCAGAGTCACAGGCAGTTCCCCAGGAAAA GCCCAGTGCCAGCTTCCTTCCAGGGCCAGGCCACTGCCTTCCTGGCTCCCTTCCCTGTCCCTGCGTCCCC AGCCCTGCGCCTTCCTTGCTATTTGGATTAAAGCCTCTGTTTTACACCTGAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_138748
• Insert Size: 972 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052852, AAH52852
• RefSeq Size: 2529 bp
• RefSeq ORF: 972 bp
• Locus ID: 110854
• UniProt ID: P58389
• Cytogenetics: 2 21.71 cM
• Gene Summary: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.