Clic4 (NM_013885) Mouse Untagged Clone

CAT#: MC202077

Clic4 (untagged) - Mouse chloride intracellular channel 4 (mitochondrial) (Clic4), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clic4
• Synonyms: D0Jmb3; mc3s5; mtCLIC; TU-74
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052890 sequence for NM_013885
  AAAGGTAATCCTGAGGATCTGAGGCCACGGGCAGCCAGACGCCGGGACTGTCCAGCGAACGAGCAAGCCA GCGAGTGAGCGAGCGGCGGGAACCCGCAGCCACAGCAGCCCCGGAGCCACAGCCGCAGCCGCCGTCCGCG GAGCCCAGCCCAACCGGCCATGGCGCTGTCGATGCCCCTGAACGGGCTGAAGGAGGAGGACAAAGAGCCC CTCATCGAGCTCTTCGTCAAGGCCGGAAGTGATGGTGAAAGCATTGGAAACTGCCCCTTTTCACAGAGGC TCTTCATGATCCTTTGGCTCAAAGGAGTCGTGTTCAGTGTCACAACCGTTGACCTGAAAAGGAAACCTGC AGATCTACAGAACTTGGCTCCTGGGACCCACCCGCCATTTATAACCTTCAACAGCGAAGTCAAGACGGAT GTGAATAAGATTGAGGAATTTCTCGAAGAAGTCTTGTGCCCACCCAAGTACCTAAAGCTTTCACCAAAGC ACCCAGAGTCAAACACTGCTGGAATGGACATCTTTGCCAAATTCTCTGCTTACATCAAGAACTCAAGACC AGAGGCTAATGAAGCGCTGGAGAGGGGGCTCTTGAAAACGCTGCAGAAACTGGATGAGTACCTCAACTCT CCCCTCCCCGACGAAATCGACGAGAACAGCATGGAGGACATCAAATTTTCCACACGTCGGTTTCTGGACG GCGATGAGATGACATTAGCAGACTGTAACCTGCTGCCCAAGCTCCACATTGTCAAGGTGGTGGCCAAAAA ATACCGCAACTTTGATATTCCTAAAGGAATGACAGGCATCTGGAGGTACCTGACAAACGCCTACAGCAGG GACGAGTTCACCAACACCTGTCCCAGCGACAAGGAGGTGGAAATCGCGTACAGCGATGTCGCCAAGAGAC TTACCAAGTAGACAGCACTTTCGAGAGAGATGCCTTCCGATCCCCCGAGAATCCGCTTTCCTAACAGACT GCTCCGCCTCCTAAAGTAGAAGTGTATTTTGCATGAACACACAGTTACTCAAGACTAGGAGTAAGGCTAG ACAGAGTAGGCCTCTCCAGTACACACTCCTGAGCAGTATTATTCTAAACTCCTCTACCCAGCCCACCTAC CCTACCCTGCCCTGCCCATCTGCTGCCCTCCAGTTTGGAGGCACTTCGCCACAAGCGTGTCACCCAAGAG GCAGTTTGCTGTCACTCTGGAGCCCCGACCATCACGTCCCTTCACTGTGTAGATGGCAGAACTTGAGGTG CTATGTTCCACCATTAGAAAAGTCGCCTGCGACATCAGTGGACCCCATGTTGCTGTGTGCATGGTATAGG AACATTTATGTACTGGGGGAGTTGTAATATTGTGTAAGGAGTCTGGAAGGATTACTACTGTGTCAGACGT GCTGTTTCAGAGTAGTCTGTTCTGGAGTGTCTGAGTGAGAAGGCCACTTCTGTAGAGACACTGGTTTCTA GGTAGACATTTAATTTTGACTAAAATGTGTCATTTGTATAAATTGTAACATCCGATAGATACTACATAGA AAATGAAGGACTGAGGGGTTAGGAAGGCATTTTCAAAATGACAAGAGAAGGTTGGGTGGGGTCTCTGTCT ATCTTGCCTGCTGTGAGGGTATCACCCAGGCCCACTTATCCATCTACAGCGAGTAGTATGGCGGCCTTCC TTGTAACAGGCTTTTTCTTTTCTCTCTTCGTGGTGCTTGGGATGGAACCCAGGGCTTTGTTTAGGCCTGA CAAGGCTCTGCCCCTGAGCTGTGCCAAGCCCACCTCCCTCTGTGTACAAAGCTCCTTTCTTGGGTGACCA ACATCTTCCTGTCTTTGAGCAACCAAGGCCAGCATGCGAGCCACCCAGAAGTTAATAAACCAGGTTCAGC GGGAGTTTGCTGAAATGTTAAGCATACAATGTTCTGGAGAGGGAGTGAAGAAGGGGCCATCTTAAACTCC CGTGAGGCCCAGGCCTCGTGCTCCTGCCCCACCTCCATGTGCATCAGACTGGGTGAGAGGTCCTTCACTT GTCTTGGGATAAATTGTAGTTGTCTTAGTGAGTGTGTATTTCCACAGTTTTGCCTCATGTCAGTCCCCAC GCGGAAGGATAGCCTTCTGTGTTCTTCTTGCCTTTGTTTTGAGACAGGATGTTACTCTGAAACCCTGGTT GGCCTGGAACCTGATAGGTGACCAAGCCAGCCTCAAACTCGTGGCAGCCCTCTTGCATTTGCCTCCTGAA TTGGAGCTAGGATTCAGCCTGCACCACCACGGCTGGCTTGTTTTCTCATCTCTAAAAACAAAGTCTAAGC TAAAAGTTGTTTGCAGTCTAACAAAGGGGAATGAGCTTTGTAAAACCAGAGGCCCCTCCCGCCCAGACAA AATGGCCTTTCTCAGTAGTACTCCAGAGCCTGCTGTGACTTTGTGTACTGTGCTACATTTTTTTTTAGTG ATCTTTTTAAAAACATGTTCAAGAAATGCTCTTTCTTAAAGTCGTCTCACTCCACAGTTCGCTGCTTTAA AGTGGCCATGCTTTCCCCTACGCTGTGCTGACACTTAAAATAAATGCCAGCTTTATGGAACCAAAAAAAA AAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013885
• Insert Size: 762 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052890, AAH52890
• RefSeq Size: 2600 bp
• RefSeq ORF: 762 bp
• Locus ID: 29876
• UniProt ID: Q9QYB1
• Cytogenetics: 4 D3
• Gene Summary: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3 (By similarity). May play a role in angiogenesis.[UniProtKB/Swiss-Prot Function]