Mettl14 (NM_201638) Mouse Untagged Clone

CAT#: MC202029

Mettl14 (untagged) - Mouse methyltransferase like 14 (Mettl14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mettl14
• Synonyms: G430022H21Rik; mKIAA1627
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052204 sequence for NM_201638
  GTGAAAGTCTCCGGGTCAGAATCTCTGCGGGGCGGGACCCGGTGTTTCCTGGTTTGGCAGGTGGATTTGC ATTTTGGCGGGCGCGGAGTCCGTCGGGGTGGTGAGCAGCCGAAGCTGGGGCATGGATAGCCGCCTGCAGG AGATCCGGGAGCGGCAGAAGTTACGGCGGCAGCTCCTAGCTCAGCAGTTGGGAGCTGAGAGTGCGGATAG CATTGGTGCTGTGTTAAATAGCAAAGATGAACAGAGGGAGATTGCTGAAACCAGAGAAACCTGCAGGGCT TCCTATGATACATCTGCTCCAAACTCAAAACGGAAGTGTCTGGATGAAGGAGAGACTGATGAAGACAAAG TAGAAGAATATAAGGATGAACTGGAAATGCAGCAGGAGGAAGAGAATTTGCCATATGAAGAAGAGATTTA CAAAGATTCCAGTACCTTTCTTAAGGGAACGCAGAGCTTAAATCCCCATAATGATTACTGCCAACATTTT GTAGACACTGGACACAGACCTCAGAATTTCATCAGGGATGTAGGTTTAGCTGACAGATTTGAAGAATACC CTAAACTTAGGGAACTCATCAGACTAAAGGATGAGCTAATAGCTAAGTCAAACACTCCTCCCATGTACTT ACAAGCAGACATAGAAGCCTTTGACATCAGAGAATTGACACCCAAATTTGATGTGATTCTCCTGGAGCCC CCTCTGGAAGAATACTATAGAGAGACTGGCATCACTGCGAATGAGAAATGCTGGACCTGGGATGATATTA TGAAGTTAGAAATCGATGAGATTGCAGCACCTCGGTCATTTATATTTCTCTGGTGCGGTTCTGGGGAAGG ATTGGACCTTGGGAGAGTATGCTTGCGAAAGTGGGGTTACAGAAGATGTGAAGATATTTGTTGGATTAAA ACCAATAAAAACAATCCTGGAAAGACAAAGACTCTAGATCCAAAGGCAGTTTTCCAGAGAACAAAGGAGC ATTGCCTGATGGGGATCAAAGGAACCGTGAAGCGAAGCACAGACGGGGACTTCATTCATGCTAATGTTGA CATTGACTTAATTATCACAGAAGAACCTGAGATTGGCAATATAGAAAAACCAGTAGAAATTTTTCATATA ATAGAACATTTTTGTCTTGGTAGAAGACGCCTTCATCTCTTTGAGAGAGATAGCACTATCAGGCCAGGCT GGCTCACAGTTGGACCAACGCTTACAAACAGTAACTACAATGCAGAAACATATGCATCGTATTTCAGTGC CCCCAACTCATACTTGACCGGATGTACAGAGGAAATCGAGAGGCTTCGACCGAAGTCACCTCCTCCCAAG TCCAAGTCTGACCGTGGGGGTGGAGCTCCCAGAGGTGGAGGAAGGGGGGGAACATCTGCTGGCCGTGGTC GGGAAAGAAACCGATCCAATTTCCGAGGAGAGAGAGGTGGCTTTAGGGGGGGCCGTGGAGGCACGCACAG AGGCGGCTTTACTCCTCGGTAGCTCTTACACTTGCATCTTTATCACAGTGGAGACTTGCTTCAGAGCTCA CTCCCAGCTTGTACTTTGCTTTAGTTTCTCATGCTGCCAGAAAGATCTTAGCCACCCCACCCCACCCCAG GGACTGGCAGTTGTCACAGACTGAGCTTTTGCTCAGGGTGGGTGAGTCACGTGTGCGCAACACCCCGACC TGACTTGACTGATTCCAGTCAAGGCTTTGGCTTCCTGGAAATGGCTGCGCAGAGGCTTCGGCATCTCAGA GGAGTGGCAGGAGTGGGTGCTGTGTTCCCTGTCATGGCTGCACGGCAGCAGGAGGCTGGGAACCCGGGTA GAACAGAATTAGAGAGGTGGTCACTGTGTTGGGCCTTTAATGTTTTCTTGTTGAAACACTAAAAAGAGAA GAGTTTTTCCCCTTACTTTTAAATTCTAAAAAATAACATGAAGTATCATTTGAGTTCCCAGAACTCGGGA GGGGAAATGAAATCTCGTTCTTCCAGATCAGAATCTGAGGTGGTTGCAGTCGTCAGGGTAGGTAACGGGA GAGGCAGGCAGAGCATGGGATATTTCAAACAAAACCAAGCTTATCATAATGTAAATTAGCGGCTACAGAT GGCTCCTGACCATGTGTCTTCAGAAGCAAGACAAGGCATGTATGTCTCCAGGTCGGAGTGTGAACCTGAT CTTGGGCTAACCTAAGATAATTCAGATTTCTTAGCAAAGGCAAAAACAGATTTGGGGAGGGGCCGGTTTA AACGGTTGAGAAAATAAAATCTTGATGATGAGGAAATGACAAACATCTTTAAAGTTGTTACTAAAGAAAA TTTAGGTAAAAGCAATTCCAGAAAACCCTTACGAATACAGCGTGGAAGAGGACCTGCAGTGTCCTTTGAT TAGAACTATGTGGTGCAGAAGTCGCCGCAGGGCTGATGGGCAGATCTCTTGGCTGTGGGAAGCTTCGTGC TGTTGCCTTTCTACACCTTAAGTCCTATTTGATGGATTTGGATATGCCAGATGAGATAATGCCAATATTG TTCTGGGTAACTCCTTTTTTATTTTGCTATAACTTTTCTAATAAAAATTTAAACCTTTGAAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_201638
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052204, AAH52204
• RefSeq Size: 2595 bp
• RefSeq ORF: 1371 bp
• Locus ID: 210529
• UniProt ID: Q3UIK4
• Cytogenetics: 3 G1
• Gene Summary: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24394384, PubMed:28965759). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (By similarity). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (By similarity). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (PubMed:24394384). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (PubMed:24394384). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (PubMed:28914256). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (PubMed:28965759).[UniProtKB/Swiss-Prot Function]