Cnot9 (NM_021383) Mouse Untagged Clone

CAT#: MC202003

Rqcd1 (untagged) - Mouse rcd1 (required for cell differentiation) homolog 1 (S. pombe) (Rqcd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cnot9
• Synonyms: 2610007F23Rik; AI593551; Fl10; Rqcd1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051948 sequence for NM_021383
  AGAACATACTTTGAGATTATTTGCTAGGCCGAGGTCCGGGAGGCGGGAAAGGGCTGGGTGGGCGGAGGGT CAGAGGTGAGAGGTCAAAGGCCCGCGAAGGGGGCGGAGCGAGGCGGAGGCGGATGGCGGCACTTCATCTT GGGCATCAGAGCCGCTGAAGCTGGGACGCGGGTCCGGTGCGTCCGGCGGGAGGAGGAGTGCGGCGGCCGC TCCAAACATGCACAGCCTGGCAACGGCAGCGCCTGTGCCTACTGCACTAGCCCAAGTGGACAGAGAGAAG ATCTATCAGTGGATCAATGAACTGTCCAGTCCTGAGACAAGGGAAAATGCTTTGTTGGAGCTGAGCAAGA AGAGAGAGTCTGTCCCTGACCTTGCACCCATGCTATGGCATTCATTTGGTACTATTGCAGCACTGTTACA GGAAATTGTAAATATTTATCCATCTATCAACCCCCCAACCTTGACAGCACACCAGTCTAACAGAGTTTGC AATGCTTTAGCATTGCTGCAGTGTGTGGCCTCACACCCGGAGACCAGGTCAGCTTTTCTGGCAGCACACA TCCCACTCTTTTTGTACCCCTTTTTGCACACAGTCAGCAAAACTCGTCCCTTTGAATATCTTCGGCTCAC CAGCCTTGGAGTTATTGGGGCCTTGGTTAAAACAGATGAGCAAGAAGTAATCAACTTTTTATTGACCACA GAGATCATCCCTCTGTGTCTGCGCATCATGGAGTCTGGAAGTGAACTCTCTAAAACGGTTGCCACATTCA TACTCCAGAAGATCCTCTTGGATGACACTGGTTTAGCTTATATATGTCAGACATATGAGCGTTTTTCCCA TGTTGCCATGATCTTGGGTAAAATGGTCCTGCAGCTATCCAAAGAACCGTCAGCCCGTCTGCTGAAGCAC GTAGTAAGATGTTACCTTCGACTCTCAGATAATCCCAGGGCACGTGAAGCACTCAGACAGTGCCTCCCTG ACCAGCTGAAGGACACAACCTTTGCCCAGGTGCTAAAAGACGACACCACCACGAAACGCTGGCTTGCACA ACTGGTGAAGAACCTGCAAGAGGGCCAGGTCACCGATCCCCGGGGGATTCCCCTGCCCCCTCAGTGATCC TCGTTCCCTCTCACTGCTCCCCTTTGCTGGGGAGTGAGGGTGGAACCTATGAGGAAAACAGCTCAGGTTT TATTGCTGGGAATAGACAACCTCAGTGCTGAACTGTACTGGAGAAAAGGGGCAAGGTACCCGCTGAGGTG TGTGGGCTGACTGGAGGTCCTGGATCCCCCTACTGCTCCCAGGAATTGGGCTCCTGACAGCAGTCTGCTA CCACAACCCCAGGCGGGTGTCAACACCAGCAAATGCTGTGTTTGCAGCATGCCCAAGATGACCCTGCTCC TGAACCTCTACCTAGCCACTGGTAGAGAGAGGAGAGAACGTGGTAATGGCAGCAACACTCTAGGCATGAG AGCACCGGGGACCAAGCCATGTGGCTTTTTTTTTTTTTTTTTAAACTTTGCCTTCCTGGACAACTTAAGG TGTGTCTCTTAATTCTCTCTTACAGTATTTGTTTACTTTGGATTTTTGTTTTTAATCTCAAAAGAGAGAG ATGTGTTTAATGGACACAAGCTGTAGTTCTCAGCAAAACTTTGTCAGTTGGCACTGTTTACAAGTCTGTT AGCTGCATAAGCTCAATAAAAAGTTGGTCTGGGCATTGCATCCCCTCTGACAGACCGATAGCTGCATCAG GCTGTTGGGTGGAATGGGAGGCAAGGGAATAGGAAAGCCAAAGGCCAAGCAGGAACTCTGTGCCTGCTCT CTGCTCCTGTTCTTTCCCTCCCATACCTGGCTGCTGTGAGGACACTCACCTTGTTCTGGCTTCAGCTGCT AGCCTACCCACATCTCAGCGCTTTCCCCTTTTTTTACCACTTAGACTTAAAGCAGGTGTCTTAATTTACT CAACCTTCCCTAAAAGAGAATTGTATTGGGGCAGGAGGGATCGCAGTTCTTCAAATTCAGGTCTTCGAAG ATAATTTCCAGGTGTCTTCTCAAACGGAGTAAGCGCCTGGATCCAAATGGTAGACTACTTGACCCCGTTC TCTAGCGTGAGTTTTCATAGTGCCATCTCCTCATGATTGTAGAAGCAGCTGAATGAGTTTCTTCAGATTT AAAATGAATAGCTGGACTCTGCTGGCTCAGTCTCCTCTCCTTTTTAACACACTTTCTTAGGTCTATGGAA ACTACAGGGTTGCCACTGAAATGTTTTTTTACTTGTTGATAGTGTCAGGGCCCCCCTTCCATCCCCATGG GCTTGACTCTCAACCTGTTACTGTAAACTGCGATCCTTCCCACCGTCAGCCTTTGTTTCTCATCCTCACC TCCAAAGATGGGATTATTAATCCAACTCTAGAAAGAGTCCAAATCTTCAGCCCGTGTCTCATACACTTGA AGATGTCTATATTGAGTTTGAAGAGGTTATAAAGGGACAGATGGTCATAGAAACCTTTGCAGAGGCAGTT GTGCCAACCCAAGGGCTCTTTCATTGTTCGTTGGCTTCTGTCTTTAAAGCCAAGAAAGTAGTAATTAAGT AGCCTAGATTTAAGGAGGGTAGAATAAGCATTTATTCCCCTGCCTTCCAAATTGAAGAGGAGGACTTGGC CTCAAGCCTGAAGAACAAAAGTCTCAGAAAGCAAAGAACAGCATCACCTTGATAAATGTCTTGACTCAGT CTGCGGCTTGTGATCTGAAAAGTTCTCTAGGGTACCCTGGTTTTTGAAATATGCCTTCCTGCTGAGCTGA AGTCTTTCATTCCACTTCACTGGGGGAGCAGCTGAGGCTTGGGGCCTTGCTCCCTGGGACATTCTCTCCA TCTTTTGGTGCATTGGCCATATTACTGTGCCAATAGTGTCTTAACTCATGTCCCATCTATCCTCAGCTGG CCTTGGACCTACATAGGAGTTGAATGGGGAGGGGCTACAATGGGTCTTCCTGTGGTTGGTCCTGATGTTG TATGTACTATATAAAGAGACCCCTCCCCTTATATTGTGTTTTCCATCCCTCACCCTCAACAGGATTAAAA TAAAACATGCTGGGTTTTTTTGTTTTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_021383
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051948, AAH51948
• RefSeq Size: 3125 bp
• RefSeq ORF: 900 bp
• Locus ID: 58184
• UniProt ID: Q9JKY0
• Cytogenetics: 1 C4
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in down-regulation of MYB- and JUN-dependent transcription. May play a role in cell differentiation. Required for retinoic acid-induced differentiation of F9 teratocarcinoma cells. Does not bind DNA by itself. Enhances ligand-dependent transcriptional activity of nuclear hormone receptors. May play a role in cell differentiation.[UniProtKB/Swiss-Prot Function]