Ephb3 (NM_010143) Mouse Untagged Clone

CAT#: MC202000

Ephb3 (untagged) - Mouse Eph receptor B3 (Ephb3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ephb3
• Synonyms: AW456895; Cek10; Etk2; HEK2; MDK5; Sek4; Tyro6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053085 sequence for NM_010143
  AGCGGCGCAGCCAGATACCGGCTCCTACCCCGGACGGCGAGCGCACAAGGATCCCGGGATCTGAGCTCGG CCTCAGTGGACTGTGCCCCGAGAACCTGCGACCACCTGCGCCCTCCGCTTCCCGAGTCATTGGGCATCAA GCCACCCAGCGCCCCGGATCCTCCTGGACCGGTCCGTCCAGATTCCCGCGGGAACGACCTGCCGGCATCT ACCCGGAGTCCCGGGTTGGGCGACACCTCGGTTACAGAGCAGCCCGTCTGCTTTGCTCCCCCTCCCTGAC GGGATCTCTTGGACTGTGCGCGAGCCTTCTTCCTCGGGCGTCCCCAGCCCCGCCGGCGCGGCCCGGCTCG GCCCCCCAACCGAGCGCCCCGGGCTATGGCCGGAGCCCGCCCGCCGCCGGGGCTTCTGCCGCTGCTCGCT CCGCTGCTGCTGCCGCTGCTGCTTCCCGCCGGCTGCTGGGCGCTGGAAGAGACTCTCATGGACACGAAAT GGGTGACGTCTGAGCTGGCATGGACATCTCATCCAGAGAGTGGGTGGGAAGAAGTAAGCGGCTATGATGA AGCCATGAATCCTATCCGCACGTATCAGGTGTGTAATGTGCGTGAGTCGAGCCAGAACAACTGGCTGCGG ACGGGTTTCATCTGGCGGCGGGAAGTCCAGCGCGTCTACGTGGAGCTGAAGTTCACCGTAAGAGACTGTA ACAGCATTCCCAACATCCCTGGCTCCTGCAAGGAAACCTTCAACCTTTTCTACTACGAGGCTGATAGCGA TGTGGCCTCAGCCTCCTCTCCCTTCTGGATGGAGAACCCCTACGTGAAAGTGGACACCATTGCGCCAGAT GAGAGCTTCTCGCGGCTCGATGCCGGGCGTGTCAACACCAAGGTGCGCAGCTTCGGGCCGCTTTCCAAGG CTGGCTTCTACTTGGCCTTCCAGGACCAAGGTGCCTGCATGTCACTCATCTCTGTGCGTGCCTTCTACAA GAAGTGTGCATCCACCACTGCAGGCTTCGCACTCTTCCCCGAGACCCTCACGGGGGCTGAGCCCACTTCG CTGGTCATTGCCCCCGGCACCTGCATCGCTAACGCTGTGGAGGTCTCTGTACCACTCAAGCTCTACTGCA ATGGCGACGGGGAGTGGATGGTGCCCGTCGGTGCCTGCACCTGCGCTACTGGCCATGAGCCAGCCGCCAA GGAGTCCCAGTGCCGTGCCTGTCCTCCTGGGAGTTACAAAGCAAAGCAAGGAGAAGGGCCCTGCCTCCCC TGTCCCCCCAATAGCCGCACCACCTCGCCGGCTGCCAGCATCTGCACCTGCCATAATAACTTCTACCGTG CAGACTCAGACTCGGCCGACAGCGCCTGCACCACGGTGCCATCTCCACCCCGGGGTGTGATCTCCAATGT GAATGAGACCTCGCTAATCCTCGAATGGAGTGAGCCCCGGGACCTTGGCGGACGAGATGACCTCCTTTAT AATGTTATCTGCAAGAAGTGCCGTGGGAGCTCTGGGGCTGGGGGTCCGGCAACCTGTTCACGCTGCGATG ACAACGTAGAGTTTGTACCTCGGCAGCTGGGCCTGACAGAGCGCCGGGTCCACATTAGCCACCTGTTGGC CCACACACGCTACACCTTTGAGGTGCAGGCTGTCAACGGTGTCTCAGGCAAAAGCCCTTTGCCACCCCGC TATGCAGCTGTGAATATCACCACCAACCAGGCTGCTCCATCAGAAGTGCCTACACTCCACCTGCACAGCA GCTCGGGGAGCAGCCTGACCCTGTCCTGGGCACCCCCAGAGCGGCCTAATGGAGTCATCTTGGACTATGA GATGAAGTACTTTGAGAAGAGTAAAGGCATCGCCTCCACTGTAACCAGCCAGAAGAACTCCGTACAACTG GACGGGCTGCAGCCTGATGCCCGCTACGTAGTTCAGGTCCGGGCTCGCACAGTAGCGGGTTACGGACAGT ATAGCCACCCGGCTGAGTTTGAGACCACAAGTGAAAGAGGTTCAGGTGCCCAGCAGCTTCAAGAACAGCT TCCCCTGATTGTGGGATCCACCGTAGCCGGGTTTGTCTTCATGGTGGTCGTCGTGGTCATCGCTCTTGTC TGCCTCAGGAAGCAGCGCCATGGCCCCGATGCAGAATACACAGAGAAGCTGCAGCAATACATTGCTCCTG GGATGAAAGTTTACATTGACCCCTTTACCTATGAGGATCCCAATGAGGCCGTCCGAGAGTTCGCCAAGGA GATCGATGTGTCCTGTGTCAAGATCGAGGAGGTGATTGGAGCTGGGGAGTTTGGGGAAGTGTGCCGGGGT CGACTGAAACTGCCCGGCCGCCGGGAGGTGTTCGTGGCTATCAAGACGCTGAAGGTGGGATACACGGAGA GGCAGCGGCGGGACTTCCTAAGTGAGGCTTCCATTATGGGTCAGTTTGACCATCCCAATATAATCCGTCT AGAGGGTGTGGTCACCAAAAGTCGTCCAGTCATGATCCTCACTGAGTTCATGGAGAACTGTGCCCTGGAC TCCTTTCTACGGCTCAATGACGGACAGTTCACGGTCATCCAGCTTGTGGGCATGTTACGAGGCATTGCTG CTGGCATGAAGTACTTGTCTGAGATGAACTACGTGCACCGTGACCTTGCTGCCCGAAACATCCTTGTCAA CAGCAACTTGGTCTGCAAAGTCTCTGACTTTGGCCTCTCCCGCTTCCTGGAGGATGACCCATCAGACCCC ACCTACACCAGCTCCCTGGGTGGGAAGATCCCTATCCGCTGGACCGCCCCGGAGGCCATAGCCTATCGGA AGTTTACGTCTGCCAGCGATGTCTGGAGCTATGGGATTGTCATGTGGGAGGTCATGAGCTACGGAGAGCG ACCCTACTGGGATATGAGCAACCAGGATGTCATTAATGCTGTAGAGCAAGACTATCGGTTACCACCCCCC ATGGACTGCCCCACCGCGCTGCACCAGCTCATGCTGGACTGTTGGGTGCGGGACCGGAACCTCAGGCCCA AGTTCTCTCAAATCGTCAACACACTAGACAAACTTATCCGCAATGCTGCCAGCCTCAAGGTCACCGCCAG TGCCCCATCTGGCATGTCCCAGCCCCTCCTGGACCGCACAGTCCCAGATTATACGACCTTCACGACAGTG GGTGACTGGCTAGATGCCATCAAGATGGGGAGGTATAAAGAGAGCTTTGTCGGTGCTGGGTTTGCATCCT TTGACCTGGTGGCCCAGATGACTGCAGAAGATCTGCTAAGGATTGGGGTCACTTTGGCTGGGCACCAGAA GAAGATCCTCTGCAGTATCCAGGACATGCGGCTGCAGATGAACCAGACCCTGCCTGTGCAGGTCTGACAC TCAGCTCCAACGAGGGGGGGGTGCCCCCCCAGGACTGCACAAGGATTCTGACCAGCCAGCTGGACTTTTG GATGCCTGGCCTCTGGCTGTGGCCCAGAAGATGGAAGTTTGGGGGAGGACCCTAGCTGTGACTTCTCCAG GCCTGTGCTCCCTCCCAGGAAGTGTGCCCCAAACCTCTTCATATTGAAGATGGATTAGAAGAGGGGGTGA TGTCCCCTCCCCAGATGCCTTGGGGCCCAGGCCTGCCTGCTCTCCAGTGGGGAGTCTTCACAACTCAGAT TGGGCTGCGCTTCAGTAATGGACGTCCTGGTAGGGTCAGGTGGGGATAAGCCTGGGTTCTTCAGGCCCCA GCCCTGGCAGGGGTCTGACCCCACCAGGTAAGCAGAGAGTACTCCCTCCCCCAGGAAGTGGAGGAGGAGA CTCTGGGGATGGGGAGATATGGTGCCCCATCCTGAAGCCAGCTGGTACCTCCAGTTTGCACAGGGACTTG ATGGGGGCTGAGGGCCCTGCCTACCCTTGGTGCTGTCATAAAAGGGCAGGCAGGAGCAGGCTGAGAAGCA GCCTGTGCCTCCCAGAGACTGACTCAGAGAGCCAGAGACTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTGTGTGTGTGAAAGACGGGGGTGGGGGTATGTATGTGTGTGTTGTGCACATGCTTGCCTGCAC AGAGAGCATGAGTGTGTACAAGCTTGGCCCTGTGCCCTGTAGTGGGGCCAGCTGGGCAGACAGCAAAATA AAGGCAATAAGATGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_010143
• Insert Size: 2982 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053085, AAH53085
• RefSeq Size: 4164 bp
• RefSeq ORF: 2982 bp
• Locus ID: 13845
• UniProt ID: P54754
• Cytogenetics: 16 B1
• Gene Summary: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.[UniProtKB/Swiss-Prot Function]