Gcat (NM_013847) Mouse Untagged Clone

CAT#: MC201977

Gcat (untagged) - Mouse glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gcat
• Synonyms: AI526977; Kbl
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024107 sequence for NM_013847
  GTCACGTGACGGGGAGCACTAGACTTCAGGCTGGCCAGCGCACTTTAGCCTGTGGGCGCGATGTGGGCCA GCTTCATGTGGCACGGGGCGCTGTCCCCTGGCCGCCGCGCACATTCAGCGCTGGCTCAGTTGCGCTGCAT CCTGGACAGCGAACTGGAAGGGATCCGCGGAGCCGGCACCTGGAAGAGTGAGCGTGTGATCACGTCCCGC CAGGGACCGAGCATCCGCGTGGACGGCATCTCGGGAGGAATCCTCAACTTCTGTGCCAATAACTACCTGG GCCTGAGCAGCCACCCTGCAGTGATCCAGGCAGGTCTGCAGACTCTGGAGGAGTTTGGAGCTGGACTCAG TTCTACTCGATTTATCTGTGGGACCCAGAGCATCCATAAGAATCTAGAAGCCAAGATAGCCCACTTCCAC CAGCGTGAGGACGCCATCCTCTATCCCAGCTGTTTCGATGCCAACGCTGGCCTATTTGAGGCTTTGCTGA CCCCAGAGGATGCGGTTCTGTCAGATGAGCTGAACCATGCTTCCATCATTGACGGCATCCGACTGTGCAA GGCCCACAAGTACCGTTACCGCCACCTGGACATGGCTGACCTGGAAGCCAAGCTGAAGGAGGCTCAGAAG CACAGGCTGCGCCTGGTGGCCACAGATGGAGCTTTCTCCATGGATGGTGACATCGCTCCCCTACAGGACA TCTGCCGCCTTGCCGCCCAATATGGTGCCCTGGTCTTTGTGGATGAATGCCATGCCACTGGCTTTCTTGG GCCCACAGGACGGGGCACAGATGAGCTGCTAGGTGTCATGGACCAGGTCACCATCATCAACTCCACCCTG GGGAAGGCGCTGGGTGGAGCATCAGGGGGATATACCACCGGGCCTGAGCCACTGGTGTCCCTGCTACGGC AGCGTTCTCGGCCCTACCTCTTCTCCAACAGCCTGCCCCCTGCTGTAGTCGGCTGTGCTTCTAAGGCCCT GGACCTGCTCATGGAGAGCAATGCCATCATCCAGTCTATGGCTGCCAAGACCCGGCGGTTCCGCAGTAAG ATGGAAGCTGCTGGTTTTACTGTCTCAGGAGCCGATCACCCCATCTGCCCTGTGATGCTGGGAGATGCAC GGTTGTCTTCTCAAATGGCAGATGACATGTTAAAGAAAGGTATCTTTGTCATCGGATTTAGCTACCCTGT GGTCCCTAAGGGCAAGGCCCGGATCCGGGTGCAGATCTCAGCTGTACACAGTGAGGAAGACATTGACCGC TGTGTGGAAGCCTTCGTGGAGGTGGGGCGGCTGCATGGAGCTCTGCCCTAAGCTCTTGGTAGGGACCTGC AGAGCCAAAGTTCCTCCACTGCCGCTGACATAGGGCTCCTCTGGTCAGCCCAGATCAGAGGCTGTGACTC AACCCAGACTTCTAGAGCCTGGTTGAAAATGGGCCTGTACTTGTCATCAGAGAGACAACAATGTGGAATT TGGCTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013847
• Insert Size: 1251 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024107, AAH24107
• RefSeq Size: 1490 bp
• RefSeq ORF: 1251 bp
• Locus ID: 26912
• UniProt ID: O88986
• Cytogenetics: 15 37.7 cM